Höbartner, Claudia

[["dc.bibliographiccitation.firstpage","373"],["dc.bibliographiccitation.issue","58"],["dc.bibliographiccitation.journal","Angewandte Chemie. International Edition"],["dc.bibliographiccitation.lastpage","379"],["dc.bibliographiccitation.volume","59"],["dc.contributor.author","Meyer, Andreas"],["dc.contributor.author","Dechert, Sebastian"],["dc.contributor.author","Dey, Surjendu"],["dc.contributor.author","Höbartner, Claudia"],["dc.contributor.author","Bennati, Marina"],["dc.date.accessioned","2019-12-09T11:04:02Z"],["dc.date.accessioned","2021-10-27T13:12:49Z"],["dc.date.available","2019-12-09T11:04:02Z"],["dc.date.available","2021-10-27T13:12:49Z"],["dc.date.issued","2019"],["dc.description.abstract","Spectroscopic and biophysical methods for structural determination at atomic resolution are fundamental in studies of biological function. Here we introduce an approach to measure molecular distances in bio-macromolecules using 19 F nuclear spins and nitroxide radicals in combination with high-frequency (94 GHz/3.4 T) electron-nuclear double resonance (ENDOR). The small size and large gyromagnetic ratio of the 19 F label enables to access distances up to about 1.5 nm with an accuracy of 0.1-1 Å. The experiment is not limited by the size of the bio-macromolecule. Performance is illustrated on synthesized fluorinated model compounds as well as spin-labelled RNA duplexes. The results demonstrate that our simple but strategic spin-labelling procedure combined with state-of-the-art spectroscopy accesses a distance range crucial to elucidate active sites of nucleic acids or proteins in the solution state."],["dc.identifier.doi","10.1002/anie.201908584"],["dc.identifier.isbn","31539187"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16871"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91724"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.eissn","1521-3773"],["dc.relation.issn","1521-3773"],["dc.relation.orgunit","Fakultät für Chemie"],["dc.rights","CC BY-NC 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.subject","RNA; fluorine; high field ENDOR; spin labelling; structural biology"],["dc.subject.ddc","540"],["dc.title","Measurement of Angstrom to Nanometer Molecular Distances with 19 F Nuclear Spins by EPR/ENDOR Spectroscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]