Butkevich, Alexey N.

[["dc.bibliographiccitation.firstpage","3290"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","3294"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Mitronova, Gyuzel Yu"],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","Klocke, Jessica L."],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Meineke, Dirk N. H."],["dc.contributor.author","D'Este, E."],["dc.contributor.author","Kraemer, Philip-Tobias"],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:54:38Z"],["dc.date.available","2017-09-07T11:54:38Z"],["dc.date.issued","2016"],["dc.description.abstract","A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of =500-630nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1m solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60nm."],["dc.identifier.doi","10.1002/anie.201511018"],["dc.identifier.gro","3141724"],["dc.identifier.isi","000371418200008"],["dc.identifier.pmid","26844929"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/369"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1521-3773"],["dc.relation.issn","1433-7851"],["dc.title","Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","ACS chemical biology"],["dc.bibliographiccitation.lastpage","6"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Ratz, Michael"],["dc.contributor.author","Stoldt, Stefan"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:21:24Z"],["dc.date.available","2018-01-17T13:21:24Z"],["dc.date.issued","2017"],["dc.description.abstract","A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells."],["dc.identifier.doi","10.1021/acschembio.7b00616"],["dc.identifier.pmid","28933823"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11709"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1554-8937"],["dc.title","Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","12114"],["dc.bibliographiccitation.issue","50"],["dc.bibliographiccitation.journal","Chemistry - A European Journal"],["dc.bibliographiccitation.lastpage","12119"],["dc.bibliographiccitation.volume","23"],["dc.bibliographiccitation.volumetitle","Special Issue Celebrating the 150th Anniversary of the Gesellschaft Deutscher Chemiker (German Chemical Society)"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Kolmakov, Kirill"],["dc.contributor.author","Sokolov, Viktor V."],["dc.contributor.author","Shojaei, Heydar"],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Matthias, Jessica"],["dc.contributor.author","Vlijm, Rifka"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:35:49Z"],["dc.date.available","2018-01-17T13:35:49Z"],["dc.date.issued","2017-09-07"],["dc.description.abstract","Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530-640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50-75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure-property relationships allow for prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogues of rhodamines, carbo-, silico-, and germanorhodamines using simple additive schemes."],["dc.identifier.doi","10.1002/chem.201701216"],["dc.identifier.pmid","28370443"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11722"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1521-3765"],["dc.title","Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1261"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Organic Letters"],["dc.bibliographiccitation.lastpage","1264"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Sednev, Maksim V."],["dc.contributor.author","Shojaei, Heydar"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-04-23T11:48:22Z"],["dc.date.available","2018-04-23T11:48:22Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1021/acs.orglett.8b00270"],["dc.identifier.gro","3142358"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13495"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","1523-7060"],["dc.title","PONy Dyes: Direct Addition of P(III) Nucleophiles to Organic Fluorophores"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3324"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Chemical Science"],["dc.bibliographiccitation.lastpage","3334"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","Mitronova, Gyuzel Y."],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Barthel, Hannah"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:46:09Z"],["dc.date.available","2022-03-01T11:46:09Z"],["dc.date.issued","2018"],["dc.description.abstract","Nanoscopy compatible fluorescent tubulin probes can be used to stain microtubules and chitin-rich taenidia in the insect tracheoles."],["dc.description.abstract","We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents – taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly ( Drosophila melanogaster ) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae."],["dc.description.abstract","Nanoscopy compatible fluorescent tubulin probes can be used to stain microtubules and chitin-rich taenidia in the insect tracheoles."],["dc.description.abstract","We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents – taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly ( Drosophila melanogaster ) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae."],["dc.identifier.doi","10.1039/C7SC05334G"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103579"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","2041-6539"],["dc.relation.issn","2041-6520"],["dc.rights.uri","http://creativecommons.org/licenses/by-nc/3.0/"],["dc.title","Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","12319"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.lastpage","15"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Mitronova, Gyuzel Y."],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Lehnart, Stephan E."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:30:31Z"],["dc.date.available","2018-01-17T13:30:31Z"],["dc.date.issued","2017"],["dc.description.abstract","Visualization of the G-protein coupled receptor (GPCR) is of great importance for studying its function in a native cell. We have synthesized a series of red-emitting fluorescent probes targeting β-adrenergic receptor (βAR) that are compatible with confocal and Stimulated Emission Depletion (STED) microscopy as well as with Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) binding assay in living cells. The probe based on the agonist BI-167107 and fluorescent dye KK114 demonstrates nanomolar binding affinity and up to nine-fold β2AR selectivity over β1AR. Carazolol-derived probes are fluorogenic and allow no-wash imaging experiments. STED microscopy of β2ARs stained at the native expression level on pancreatic CAPAN cells provides two-fold improvement in lateral optical resolution over confocal mode and reveals the formation of receptor microdomains. These probes retain their functional (agonist or antagonist) properties, allowing simultaneous modulation of cyclic adenosine monophosphate (cAMP) levels and receptor internalization as well as imaging receptor localization."],["dc.identifier.doi","10.1038/s41598-017-12468-3"],["dc.identifier.pmid","28951558"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14939"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11716"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","High-Affinity Functional Fluorescent Ligands for Human β-Adrenoceptors"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]