Butkevich, Alexey N.

[["dc.bibliographiccitation.firstpage","18917"],["dc.bibliographiccitation.issue","47"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","18917"],["dc.bibliographiccitation.volume","141"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:45:43Z"],["dc.date.available","2022-03-01T11:45:43Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1021/jacs.9b11892"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103426"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1520-5126"],["dc.relation.iserratumof","/handle/2/103424"],["dc.relation.issn","0002-7863"],["dc.title","Correction to “Triarylmethane Fluorophores Resistant to Oxidative Photobluing”"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","erratum_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","12378"],["dc.bibliographiccitation.issue","36"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","12381"],["dc.bibliographiccitation.volume","139"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","D'Este, Elisa"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-04-23T11:48:24Z"],["dc.date.available","2018-04-23T11:48:24Z"],["dc.date.issued","2017"],["dc.description.abstract","We designed cell-permeant red-emitting fluorescent dye labels with >140 nm Stokes shifts based on 9-iminoanthrone, 9-imino-10-silaxanthone, and 9-imino-10-germaxanthone fluorophores. The corresponding probes selectively targeting mitochondria, lysosomes, and F-actin demonstrate low toxicity and enable stimulated emission depletion (STED) nanoscopy in neurons, human fibroblasts, U2OS, and HeLa cells. In combination with known small Stokes shift dyes, our probes allow live-cell three-color STED nanoscopy of endogenous targets on popular setups with 775 nm STED wavelength."],["dc.identifier.doi","10.1021/jacs.7b06412"],["dc.identifier.gro","3142363"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13502"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","0002-7863"],["dc.title","Cell-Permeant Large Stokes Shift Dyes for Transfection-Free Multicolor Nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","981"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","989"],["dc.bibliographiccitation.volume","141"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:45:43Z"],["dc.date.available","2022-03-01T11:45:43Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1021/jacs.8b11036"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103424"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1520-5126"],["dc.relation.haserratum","/handle/2/103426"],["dc.relation.issn","0002-7863"],["dc.title","Triarylmethane Fluorophores Resistant to Oxidative Photobluing"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3324"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Chemical Science"],["dc.bibliographiccitation.lastpage","3334"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","Mitronova, Gyuzel Y."],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Barthel, Hannah"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:46:09Z"],["dc.date.available","2022-03-01T11:46:09Z"],["dc.date.issued","2018"],["dc.description.abstract","Nanoscopy compatible fluorescent tubulin probes can be used to stain microtubules and chitin-rich taenidia in the insect tracheoles."],["dc.description.abstract","We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents – taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly ( Drosophila melanogaster ) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae."],["dc.description.abstract","Nanoscopy compatible fluorescent tubulin probes can be used to stain microtubules and chitin-rich taenidia in the insect tracheoles."],["dc.description.abstract","We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents – taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly ( Drosophila melanogaster ) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae."],["dc.identifier.doi","10.1039/C7SC05334G"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103579"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","2041-6539"],["dc.relation.issn","2041-6520"],["dc.rights.uri","http://creativecommons.org/licenses/by-nc/3.0/"],["dc.title","Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]