Butkevich, Alexey N.

[["dc.bibliographiccitation.firstpage","3290"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","3294"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Mitronova, Gyuzel Yu"],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","Klocke, Jessica L."],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Meineke, Dirk N. H."],["dc.contributor.author","D'Este, E."],["dc.contributor.author","Kraemer, Philip-Tobias"],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:54:38Z"],["dc.date.available","2017-09-07T11:54:38Z"],["dc.date.issued","2016"],["dc.description.abstract","A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of =500-630nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1m solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60nm."],["dc.identifier.doi","10.1002/anie.201511018"],["dc.identifier.gro","3141724"],["dc.identifier.isi","000371418200008"],["dc.identifier.pmid","26844929"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/369"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1521-3773"],["dc.relation.issn","1433-7851"],["dc.title","Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","18388"],["dc.bibliographiccitation.issue","44"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","18393"],["dc.bibliographiccitation.volume","143"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Weber, Michael"],["dc.contributor.author","Cereceda Delgado, Angel R."],["dc.contributor.author","Ostersehlt, Lynn M."],["dc.contributor.author","D’Este, Elisa"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:45:42Z"],["dc.date.available","2022-03-01T11:45:42Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1021/jacs.1c09999"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103422"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1520-5126"],["dc.relation.issn","0002-7863"],["dc.title","Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","ACS chemical biology"],["dc.bibliographiccitation.lastpage","6"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Ratz, Michael"],["dc.contributor.author","Stoldt, Stefan"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:21:24Z"],["dc.date.available","2018-01-17T13:21:24Z"],["dc.date.issued","2017"],["dc.description.abstract","A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells."],["dc.identifier.doi","10.1021/acschembio.7b00616"],["dc.identifier.pmid","28933823"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11709"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1554-8937"],["dc.title","Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","18917"],["dc.bibliographiccitation.issue","47"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","18917"],["dc.bibliographiccitation.volume","141"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:45:43Z"],["dc.date.available","2022-03-01T11:45:43Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1021/jacs.9b11892"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103426"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1520-5126"],["dc.relation.iserratumof","/handle/2/103424"],["dc.relation.issn","0002-7863"],["dc.title","Correction to “Triarylmethane Fluorophores Resistant to Oxidative Photobluing”"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","erratum_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","12378"],["dc.bibliographiccitation.issue","36"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","12381"],["dc.bibliographiccitation.volume","139"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","D'Este, Elisa"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-04-23T11:48:24Z"],["dc.date.available","2018-04-23T11:48:24Z"],["dc.date.issued","2017"],["dc.description.abstract","We designed cell-permeant red-emitting fluorescent dye labels with >140 nm Stokes shifts based on 9-iminoanthrone, 9-imino-10-silaxanthone, and 9-imino-10-germaxanthone fluorophores. The corresponding probes selectively targeting mitochondria, lysosomes, and F-actin demonstrate low toxicity and enable stimulated emission depletion (STED) nanoscopy in neurons, human fibroblasts, U2OS, and HeLa cells. In combination with known small Stokes shift dyes, our probes allow live-cell three-color STED nanoscopy of endogenous targets on popular setups with 775 nm STED wavelength."],["dc.identifier.doi","10.1021/jacs.7b06412"],["dc.identifier.gro","3142363"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13502"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","0002-7863"],["dc.title","Cell-Permeant Large Stokes Shift Dyes for Transfection-Free Multicolor Nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","627"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Nature Photonics"],["dc.bibliographiccitation.lastpage","627"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Weber, Michael"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Stoldt, Stefan"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Mihaila, Tiberiu S."],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:46:00Z"],["dc.date.available","2022-03-01T11:46:00Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1038/s41566-021-00816-9"],["dc.identifier.pii","816"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103523"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1749-4893"],["dc.relation.iserratumof","/handle/2/82664"],["dc.relation.issn","1749-4885"],["dc.rights.uri","https://www.springer.com/tdm"],["dc.title","Author Correction: MINSTED fluorescence localization and nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","erratum_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","46492"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Winter, Franziska R."],["dc.contributor.author","Loidolt, Maria"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:38:46Z"],["dc.date.available","2018-01-17T13:38:46Z"],["dc.date.issued","2017-04-18"],["dc.description.abstract","The extension of fluorescence nanoscopy to larger numbers of molecular species concurrently visualized by distinct markers is of great importance for advanced biological applications. To date, up to four markers had been distinguished in STED experiments featuring comparatively elaborate imaging schemes and optical setups, and exploiting various properties of the fluorophores. Here we present a simple yet versatile STED design for multicolour imaging below the diffraction limit. A hyperspectral detection arrangement (hyperSTED) collects the fluorescence in four spectral channels, allowing the separation of four markers with only one excitation wavelength and a single STED beam. Unmixing of the different marker signals based on the simultaneous readout of all channels is performed with a non-negative matrix factorization algorithm. We illustrate the approach showing four-colour nanoscopy of fixed and living cellular samples."],["dc.identifier.doi","10.1038/srep46492"],["dc.identifier.pmid","28417977"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11725"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","2045-2322"],["dc.title","Multicolour nanoscopy of fixed and living cells with a single STED beam and hyperspectral detection"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1013"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Nature Chemistry"],["dc.bibliographiccitation.lastpage","1020"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Lincoln, Richard"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Remmel, Michael"],["dc.contributor.author","D’Este, Elisa"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-09-01T09:50:04Z"],["dc.date.available","2022-09-01T09:50:04Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\n The controlled switching of fluorophores between non-fluorescent and fluorescent states is central to every super-resolution fluorescence microscopy (nanoscopy) technique, and the exploration of radically new switching mechanisms remains critical to boosting the performance of established, as well as emerging super-resolution methods. Photoactivatable dyes offer substantial improvements to many of these techniques, but often rely on photolabile protecting groups that limit their applications. Here we describe a general method to transform 3,6-diaminoxanthones into caging-group-free photoactivatable fluorophores. These photoactivatable xanthones (PaX) assemble rapidly and cleanly into highly fluorescent, photo- and chemically stable pyronine dyes upon irradiation with light. The strategy is extendable to carbon- and silicon-bridged xanthone analogues, yielding a family of photoactivatable labels spanning much of the visible spectrum. Our results demonstrate the versatility and utility of PaX dyes in fixed and live-cell labelling for conventional microscopy, as well as the coordinate-stochastic and deterministic nanoscopies STED, PALM and MINFLUX."],["dc.identifier.doi","10.1038/s41557-022-00995-0"],["dc.identifier.pii","995"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113615"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","1755-4349"],["dc.relation.issn","1755-4330"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","A general design of caging-group-free photoactivatable fluorophores for live-cell nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","12114"],["dc.bibliographiccitation.issue","50"],["dc.bibliographiccitation.journal","Chemistry - A European Journal"],["dc.bibliographiccitation.lastpage","12119"],["dc.bibliographiccitation.volume","23"],["dc.bibliographiccitation.volumetitle","Special Issue Celebrating the 150th Anniversary of the Gesellschaft Deutscher Chemiker (German Chemical Society)"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Kolmakov, Kirill"],["dc.contributor.author","Sokolov, Viktor V."],["dc.contributor.author","Shojaei, Heydar"],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Matthias, Jessica"],["dc.contributor.author","Vlijm, Rifka"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:35:49Z"],["dc.date.available","2018-01-17T13:35:49Z"],["dc.date.issued","2017-09-07"],["dc.description.abstract","Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530-640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50-75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure-property relationships allow for prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogues of rhodamines, carbo-, silico-, and germanorhodamines using simple additive schemes."],["dc.identifier.doi","10.1002/chem.201701216"],["dc.identifier.pmid","28370443"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11722"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1521-3765"],["dc.title","Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1261"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Organic Letters"],["dc.bibliographiccitation.lastpage","1264"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Sednev, Maksim V."],["dc.contributor.author","Shojaei, Heydar"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-04-23T11:48:22Z"],["dc.date.available","2018-04-23T11:48:22Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1021/acs.orglett.8b00270"],["dc.identifier.gro","3142358"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13495"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","1523-7060"],["dc.title","PONy Dyes: Direct Addition of P(III) Nucleophiles to Organic Fluorophores"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]