Salditt, Tim

[["dc.bibliographiccitation.firstpage","24"],["dc.bibliographiccitation.issue","S2"],["dc.bibliographiccitation.journal","Microscopy and Microanalysis"],["dc.bibliographiccitation.lastpage","25"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Robisch, Anna Lena"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-03-04T13:40:15Z"],["dc.date.available","2020-03-04T13:40:15Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1017/S1431927618012540"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63109"],["dc.language.iso","en"],["dc.relation.issn","1431-9276"],["dc.relation.issn","1435-8115"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","3d Virtual Histology of Human Cerebellum by Propagation-Based X-Ray Phase-Contrast Tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Müller, Bert"],["dc.contributor.editor","Wang, Ge"],["dc.date.accessioned","2022-02-16T14:12:05Z"],["dc.date.available","2022-02-16T14:12:05Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1117/12.2596133"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/99930"],["dc.relation.conference","SPIE Optical Engineering + Applications"],["dc.relation.eventend","2021-08-05"],["dc.relation.eventlocation","San Diego, California"],["dc.relation.eventstart","2021-08-01"],["dc.relation.isbn","9781510645189"],["dc.relation.isbn","9781510645196"],["dc.relation.ispartof","Developments in X-Ray Tomography XIII"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","biomedical tomography"],["dc.title","Towards correlative imaging of neuronal tissue by phase-contrast x-ray tomography and SEM"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1261"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Scandinavian Journal of Gastroenterology"],["dc.bibliographiccitation.lastpage","1267"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Peruzzi, Niccolò"],["dc.contributor.author","Veress, Béla"],["dc.contributor.author","Dahlin, Lars B."],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Andersson, Mariam"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Robisch, Anna-Lena"],["dc.contributor.author","Bech, Martin"],["dc.contributor.author","Ohlsson, Bodil"],["dc.date.accessioned","2021-04-14T08:23:03Z"],["dc.date.available","2021-04-14T08:23:03Z"],["dc.date.issued","2020"],["dc.description.abstract","Objectives: Light microscopical analysis in two dimensions, combined with immunohistochemistry, is\r\npresently the gold standard to describe the enteric nervous system (ENS). Our aim was to assess the\r\nusefulness of three-dimensional (3D) imaging by X-ray phase-contrast tomography in evaluating the\r\nENS of the human bowel.\r\nMaterial and methods: Myenteric ganglia were identified in full-thickness biopsies of the ileum and\r\ncolon by hematoxylin & eosin staining. A1-mm biopsy punch was taken from the paraffin blocks and\r\nplaced into a KaptonVR tube for subsequent tomographic investigation. The samples were scanned,\r\nwithout further preparation, using phase-contrast tomography at two different scales: overview scans\r\n(performed with laboratory setups), which allowed localization of the nervous tissue ( 1mm effective\r\nvoxel size); and high-resolution scans (performed with a synchrotron endstation), which imaged localized\r\nregions of 320x320x320 mm3 (176 nm effective voxel size).\r\nResults: The contrast allowed us to follow the shape and the size changes of the ganglia, as well as\r\nto study their cellular components together with the cells and cellular projections of the periganglional\r\nspace. Furthermore, it was possible to show the 3D network of the myenteric plexus and to quantify\r\nits volume within the samples.\r\nConclusions: Phase-contrast X-ray tomography can be applied for volume analyses of the human ENS\r\nand to study tissue components in unstained paraffin-embedded tissue biopsies. This technique could\r\npotentially be used to study disease mechanisms, and to compare healthy and diseased tissues in clinical\r\nresearch."],["dc.identifier.doi","10.1080/00365521.2020.1815079"],["dc.identifier.pmid","32907418"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80786"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/70"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1502-7708"],["dc.relation.issn","0036-5521"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 3.0"],["dc.subject.gro","biomedical tomography"],["dc.title","3D analysis of the myenteric plexus of the human bowel by X-ray phase-contrast tomography – a future method?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Westermeier, Fabian"],["dc.contributor.author","Tzankov, Alexandar"],["dc.contributor.author","Werlein, Christopher"],["dc.contributor.author","Kuehnel, Mark"],["dc.contributor.author","Jonigk, Danny"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-06-26T11:04:13Z"],["dc.date.available","2020-06-26T11:04:13Z"],["dc.date.issued","2020"],["dc.description.abstract","We present a new approach of three-dimensional (3d) virtual histology and patho-histology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal-architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we can propose multi-scale phase contrast x-ray tomography as a novel tool to unravel the patho-physiology of Covid-19, extending conventional histology by a third dimension and allowing for a full quantification of tissue remodeling.By combining parallel and cone beam geometry, autopsy samples with a cross section of 4mm are scanned and reconstructed at a resolution and image quality which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry, regions-of-interest are reconstructed with a minimum voxel size of 160nm. We exemplify the capability of this approach by 3d visualisation of the diffuse alveolar damage with its prominent hyaline membrane formation, by mapping the 3d distribution and density of lymphocytes infiltrating the tissue, and by providing histograms of characteristic distances from tissue interior to the closest air compartment."],["dc.identifier.doi","10.1101/2020.06.21.20134882"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66752"],["dc.language.iso","en"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.subject.gro","other"],["dc.title","3d Virtual Patho-Histology of Lung Tissue from Covid-19 Patients based on Phase Contrast X-ray Tomography"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","111130N"],["dc.bibliographiccitation.firstpage","21"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Ruhwedel, Torben"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Müller-Myhsok, Bertram"],["dc.contributor.editor","Wang, Geng"],["dc.date.accessioned","2020-03-10T15:20:27Z"],["dc.date.available","2020-03-10T15:20:27Z"],["dc.date.issued","2019"],["dc.description.abstract","In the present work, we evaluate and compare the contrast and resolution obtained on different neuronal tissues with propagation-based x-ray phase contrast computed tomography (PB-CT). At our laboratory-based liquid metal-jet setup, we obtain overview datasets at sub-micron resolution of mm3 -sized volumes. In order to evaluate these parameters down to the sub-cellular level, we utilize the synchrotron endstation GINIX at P10, DESY. At this dedicated endstation1 developed and operated by our group, we utilize x-ray waveguide optics for highresolution cone-beam scans at strong geometrical magnification M. Exploiting this multi-scale approach, we investigate the image quality of cerebellum tissue treated by different heavy-metal stains. In addition, we study the electron density contrast in unstained tissues. Different embedding media are utilized depending on the stain, which also significantly affects contrast and image quality. With this work, we want to contribute to an optimized sample preparation to study the neuronal architecture of the brain tissue in greater detail in three dimensions (3d). © (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only."],["dc.identifier.doi","10.1117/12.2528432"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63286"],["dc.language.iso","en"],["dc.relation.eventend","2019-08-15"],["dc.relation.eventlocation","San Diego, California, United States"],["dc.relation.eventstart","2019-08-11"],["dc.relation.isbn","978-1-5106-2919-6"],["dc.relation.isbn","978-1-5106-2920-2"],["dc.relation.ispartof","Proc. SPIE 11113"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","biomedical tomography"],["dc.title","Evaluation of different heavy-metal stains and embedding media for phase contrast tomography of neuronal tissue"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e2113835118"],["dc.bibliographiccitation.issue","48"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Schmitzer, Bernhard"],["dc.contributor.author","van der Meer, Franziska"],["dc.contributor.author","Franz, Jonas"],["dc.contributor.author","Hansen, Ove"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2022-01-11T14:05:50Z"],["dc.date.available","2022-01-11T14:05:50Z"],["dc.date.issued","2021"],["dc.description.abstract","We have studied the three-dimensional (3D) cytoarchitecture of the human hippocampus in neuropathologically healthy and Alzheimer’s disease (AD) individuals, based on phase-contrast X-ray computed tomography of postmortem human tissue punch biopsies. In view of recent findings suggesting a nuclear origin of AD, we target in particular the nuclear structure of the dentate gyrus (DG) granule cells. Tissue samples of 20 individuals were scanned and evaluated using a highly automated approach of measurement and analysis, combining multiscale recordings, optimized phase retrieval, segmentation by machine learning, representation of structural properties in a feature space, and classification based on the theory of optimal transport. Accordingly, we find that the prototypical transformation between a structure representing healthy granule cells and the pathological state involves a decrease in the volume of granule cell nuclei, as well as an increase in the electron density and its spatial heterogeneity. The latter can be explained by a higher ratio of heterochromatin to euchromatin. Similarly, many other structural properties can be derived from the data, reflecting both the natural polydispersity of the hippocampal cytoarchitecture between different individuals in the physiological context and the structural effects associated with AD pathology."],["dc.identifier.doi","10.1073/pnas.2113835118"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97758"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/369"],["dc.identifier.url","https://rdp.sfb274.de/literature/publications/53"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","TRR 274: Checkpoints of Central Nervous System Recovery"],["dc.relation","TRR 274 | B01: The role of inflammatory cytokine signaling for efficient remyelination in multiple sclerosis"],["dc.relation.eissn","1091-6490"],["dc.relation.issn","0027-8424"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Stadelmann-Nessler"],["dc.rights","CC BY-NC-ND 4.0"],["dc.subject.gro","biomedical tomography"],["dc.title","Three-dimensional virtual histology of the human hippocampus based on phase-contrast computed tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Skin Research & Technology"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Peruzzi, Niccolò"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Bech, Martin"],["dc.contributor.author","Englund, Elisabet"],["dc.contributor.author","Veress, Béla"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Dahlin, Lars B."],["dc.contributor.author","Ohlsson, Bodil"],["dc.date.accessioned","2021-04-14T08:31:27Z"],["dc.date.available","2021-04-14T08:31:27Z"],["dc.date.issued","2020"],["dc.description.abstract","BackgroundEnteric neuropathy is described in most patients with gastrointestinal dysmotility and may be found together with reduced intraepidermal nerve fiber density (IENFD). The aim of this pilot study was to assess whether three-dimensional (3d) imaging of skin biopsies could be used to examine various tissue components in patients with gastrointestinal dysmotility.Material and methodsFour dysmotility patients of different etiology and two healthy volunteers were included. From each subject, two 3-mm punch skin biopsies were stained with antibodies against protein gene product 9.5 or evaluated as a whole with two X-ray phase-contrast computed tomography (CT) setups, a laboratory µCT setup and a dedicated synchrotron radiation nanoCT end-station.ResultsTwo patients had reduced IENFD, and two normal IENFD, compared with controls. µCT and X-ray phase-contrast holographic nanotomography scanned whole tissue specimens, with optional high-resolution scans revealing delicate structures, without differentiation of various fibers and cells. Irregular architecture of dermal fibers was observed in the patient with Ehlers-Danlos syndrome and the patient with idiopathic dysmotility showed an abundance of mesenchymal ground substance.Conclusions3d phase-contrast tomographic imaging may be useful to illustrate traits of connective tissue dysfunction in various organs and to demonstrate whether disorganized dermal fibers could explain organ dysfunction."],["dc.identifier.doi","10.1111/srt.12974"],["dc.identifier.pmid","33022848"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83598"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/82"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1600-0846"],["dc.relation.issn","0909-752X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.subject.gro","biomedical tomography"],["dc.title","3d phase‐contrast nanotomography of unstained human skin biopsies may identify morphological differences in the dermis and epidermis between subjects"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e60408"],["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Westermeier, Fabian"],["dc.contributor.author","Tzankov, Alexandar"],["dc.contributor.author","Werlein, Christopher"],["dc.contributor.author","Kühnel, Mark"],["dc.contributor.author","Jonigk, Danny"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2021-03-01T08:45:14Z"],["dc.date.available","2021-03-01T08:45:14Z"],["dc.date.issued","2020"],["dc.description.abstract","We present a three-dimensional (3D) approach for virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we propose multi-scale phase contrast x-ray tomography as a tool to unravel the pathophysiology of Covid-19, extending conventional histology by a third dimension and allowing for full quantification of tissue remodeling. By combining parallel and cone beam geometry, autopsy samples with a maximum cross section of 8 mm are scanned and reconstructed at a resolution and image quality, which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry, regions-of-interest are reconstructed with a minimum voxel size of 167 nm. We exemplify the capability of this approach by 3D visualization of diffuse alveolar damage (DAD) with its prominent hyaline membrane formation, by mapping the 3D distribution and density of lymphocytes infiltrating the tissue, and by providing histograms of characteristic distances from tissue interior to the closest air compartment."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2020"],["dc.identifier.doi","10.7554/eLife.60408"],["dc.identifier.pmid","32815517"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17619"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/79864"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/162"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2050-084X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.subject.gro","biomedical tomography"],["dc.title","3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7582"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Biomedical Optics Express"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","van der Meer, Franziska"],["dc.contributor.author","Cloetens, Peter"],["dc.contributor.author","Ruhwedel, Torben"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2022-01-11T14:06:10Z"],["dc.date.available","2022-01-11T14:06:10Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1364/BOE.434885"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97842"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/362"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/142"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A02: Bestimmung der Struktur synaptischer Organellen durch Röntgenbeugungs- und Bildgebungsverfahren"],["dc.relation.eissn","2156-7085"],["dc.relation.issn","2156-7085"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Möbius"],["dc.relation.workinggroup","RG Stadelmann-Nessler"],["dc.subject.gro","biomedical tomography"],["dc.title","Three-dimensional virtual histology of the cerebral cortex based on phase-contrast X-ray tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","013501"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Medical Imaging"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Robisch, Anna Lena"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Meer, Franziska van der"],["dc.contributor.author","Stadelmann-Nessler, Christine"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-03-04T13:21:36Z"],["dc.date.available","2020-03-04T13:21:36Z"],["dc.date.issued","2020"],["dc.description.abstract","X-ray cone-beam holotomography of unstained tissue from the human central nervous system reveals details down to subcellular length scales. This visualization of variations in the electron density of the sample is based on phase-contrast techniques using intensities formed by self-interference of the beam between object and detector. Phase retrieval inverts diffraction and overcomes the phase problem by constraints such as several measurements at different Fresnel numbers for a single projection. Therefore, the object-to-detector distance (defocus) can be varied. However, for cone-beam geometry, changing defocus changes magnification, which can be problematic in view of image processing and resolution. Alternatively, the photon energy can be altered (multi-E). Far from absorption edges, multi-E data yield the wavelength-independent electron density. We present the multi-E holotomography at the Göttingen Instrument for Nano-Imaging with X-Rays (GINIX) setup of the P10 beamline at Deutsches Elektronen-Synchrotron. The instrument is based on a combined optics of elliptical mirrors and an x-ray waveguide positioned in the focal plane for further coherence, spatial filtering, and high numerical aperture. Previous results showed the suitability of this instrument for nanoscale tomography of unstained brain tissue. We demonstrate that upon energy variation, the focal spot is stable enough for imaging. To this end, a double-crystal monochromator and automated alignment routines are required. Three tomograms of human brain tissue were recorded and jointly analyzed using phase retrieval based on the contrast transfer function formalism generalized to multiple photon energies. Variations of the electron density of the sample are successfully reconstructed."],["dc.identifier.doi","10.1117/1.JMI.7.1.013501"],["dc.identifier.pmid","32016134"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63102"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/20"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","2329-4302"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Stadelmann-Nessler"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Nanoscale x-ray holotomography of human brain tissue with phase retrieval based on multienergy recordings"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]