Salditt, Tim

[["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Neubauer, Heike"],["dc.contributor.author","Krüger, Sven P"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Mai, Dong-Du"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Hartmann, Britta"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","McNulty, Ian"],["dc.contributor.author","Eyberger, Catherine"],["dc.contributor.author","Lai, Barry"],["dc.date.accessioned","2017-09-07T11:54:07Z"],["dc.date.available","2017-09-07T11:54:07Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1063/1.3625313"],["dc.identifier.gro","3145117"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2818"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","AIP Publishing"],["dc.publisher.place","Melville, NY"],["dc.relation","SFB 755: Nanoscale Photonic Imaging"],["dc.relation.conference","10th International Conference on X-Ray Microscopy"],["dc.relation.eventend","2010-08-20"],["dc.relation.eventlocation","Chicago, Illinois"],["dc.relation.eventstart","2010-08-15"],["dc.relation.isbn","0-7354-0925-0"],["dc.relation.isbn","978-0-7354-0925-5"],["dc.relation.ispartof","The 10th International Conference on X-Ray Microscopy"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","The Göttingen Holography Endstation of Beamline P10 at PETRA III∕DESY"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","035008"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","New Journal of Physics"],["dc.bibliographiccitation.volume","12"],["dc.contributor.affiliation","Giewekemeyer, K;"],["dc.contributor.affiliation","Neubauer, H;"],["dc.contributor.affiliation","Kalbfleisch, S;"],["dc.contributor.affiliation","Krüger, S P;"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Neubauer, Heike"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Krueger, S. P."],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:46:08Z"],["dc.date.available","2017-09-07T11:46:08Z"],["dc.date.issued","2010"],["dc.date.updated","2022-02-09T21:48:01Z"],["dc.description.abstract","We report on lensless nanoscale imaging using x-ray waveguides as ultra-small sources for quasi-point-like illumination. We first give a brief account of the basic optical setup, an overview of the progress in waveguide fabrication and characterization, as well as the basics of image formation. We then compare one-step holographic and iterative ptychographic reconstruction, both for simulated and experimental data collected on samples illuminated by waveguided beams. We demonstrate that scanning the sample with partial overlap can substantially improve reconstruction quality in holographic imaging, and that divergent beams make efficient use of the limited dynamic range of current detectors, regardless of the reconstruction scheme. Among different experimental settings presented, smallest source dimensions of 29 nm (horizontal) x 17 nm have been achieved, using multi-modal interference effects. These values have been determined by ptychographic reconstruction of a Ta test structure at 17.5 keV and have been corroborated by simulations of field propagation inside the waveguide."],["dc.identifier.doi","10.1088/1367-2630/12/3/035008"],["dc.identifier.eissn","1367-2630"],["dc.identifier.fs","568205"],["dc.identifier.gro","3142948"],["dc.identifier.isi","000276349600007"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6673"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/408"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1367-2630"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","Holographic and diffractive x-ray imaging using waveguides as quasi-point sources"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Neubauer, Heike"],["dc.contributor.author","Krüger, Sven P"],["dc.contributor.author","Hartmann, Britta"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Leupold, O."],["dc.contributor.author","Siewert, F."],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Garrett, R."],["dc.contributor.author","Gentle, I."],["dc.contributor.author","Nugent, K."],["dc.contributor.author","Wilkins, S."],["dc.date.accessioned","2017-09-07T11:54:07Z"],["dc.date.available","2017-09-07T11:54:07Z"],["dc.date.issued","2010"],["dc.identifier.doi","10.1063/1.3463233"],["dc.identifier.gro","3145119"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2820"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","AIP Publishing"],["dc.publisher.place","Melville, NY"],["dc.relation","SFB 755: Nanoscale Photonic Imaging"],["dc.relation.conference","10th International Conference on Synchrotron Radiation Instrumentation"],["dc.relation.eventend","2009-10-02"],["dc.relation.eventlocation","Melbourne, Australia"],["dc.relation.eventstart","2009-09-27"],["dc.relation.isbn","978-0-7354-0782-4"],["dc.relation.ispartof","SRI 2009: the 10th International Conference on Synchrotron Radiation Instrumentation"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","The holography endstation of beamline P10 at PETRA III"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9656"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","9675"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Krueger, S. P."],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Neubauer, Heike"],["dc.contributor.author","Sprung, Michael"],["dc.date.accessioned","2020-11-05T15:05:24Z"],["dc.date.available","2020-11-05T15:05:24Z"],["dc.date.issued","2011"],["dc.description.abstract","We have studied the spatial coherence properties of a nano-focused x-ray beam by grating (Talbot) interferometry in projection geometry. The beam is focused by a fixed curvature mirror system optimized for high flux density under conditions of partial coherence. The spatial coherence of the divergent exit wave emitted from the mirror focus is measured by Talbot interferometry The results are compared to numerical calculations of coherence propagation. In view of imaging applications, the magnified in-line image of a test pattern formed under conditions of partial coherence is analyzed quantitatively. Finally, additional coherence filtering by use of x-ray waveguides is demonstrated. By insertion of x-ray waveguides, the beam diameter can be reduced from typical values of 200 nm to values below 15 nm. In proportion to the reduction in the focal spot size, the numerical aperture (NA) of the projection imaging system is increased, as well as the coherence length, as quantified by grating interferometry. (C) 2011 Optical Society of America"],["dc.identifier.doi","10.1364/OE.19.009656"],["dc.identifier.gro","3142728"],["dc.identifier.isi","000290490200090"],["dc.identifier.pmid","21643224"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7504"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68458"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-352.6"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 755: Nanoscale Photonic Imaging"],["dc.relation.eissn","1094-4087"],["dc.relation.issn","1094-4087"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","Partially coherent nano-focused x-ray radiation characterized by Talbot interferometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","023804"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Physical Review A"],["dc.bibliographiccitation.volume","83"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Krueger, S. P."],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Beta, C."],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:44:21Z"],["dc.date.available","2017-09-07T11:44:21Z"],["dc.date.issued","2011"],["dc.description.abstract","We have used x-ray waveguides as highly confining optical elements for nanoscale imaging of unstained biological cells using the simple geometry of in-line holography. The well-known twin-image problem is effectively circumvented by a simple and fast iterative reconstruction. The algorithm which combines elements of the classical Gerchberg-Saxton scheme and the hybrid-input-output algorithm is optimized for phase-contrast samples, well-justified for imaging of cells at multi-keV photon energies. The experimental scheme allows for a quantitative phase reconstruction from a single holographic image without detailed knowledge of the complex illumination function incident on the sample, as demonstrated for freeze-dried cells of the eukaryotic amoeba Dictyostelium discoideum. The accessible resolution range is explored by simulations, indicating that resolutions on the order of 20 nm are within reach applying illumination times on the order of minutes at present synchrotron sources."],["dc.identifier.doi","10.1103/PhysRevA.83.023804"],["dc.identifier.gro","3142778"],["dc.identifier.isi","000287029900011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/219"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1050-2947"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","X-ray propagation microscopy of biological cells using waveguides as a quasipoint source"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","10"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Optical Nanoscopy"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Krüger, Sven P"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Olendrowitz, Christian"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:54:07Z"],["dc.date.available","2017-09-07T11:54:07Z"],["dc.date.issued","2012"],["dc.description.abstract","We have imaged the three-dimensional density distribution of unstained and unsliced, freeze-dried cells of the gram-positive bacterium Deinococcus radiodurans by tomographic x-ray propagation microscopy, i.e. projection tomography with phase contrast formation by free space propagation. The work extends previous x-ray imaging of biological cells in the simple in-line holography geometry to full three-dimensional reconstruction, based on a fast iterative phase reconstruction algorithm which circumvents the usual twin-image problem. The sample is illuminated by the highly curved wave fronts emitted from a virtual quasi-point source with 10 nm cross section, realized by two crossed x-ray waveguides. The experimental scheme allows for a particularly dose efficient determination of the 3D density distribution in the cellular structure."],["dc.identifier.doi","10.1186/2192-2853-1-10"],["dc.identifier.fs","593648"],["dc.identifier.gro","3145116"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9581"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2817"],["dc.language.iso","en"],["dc.notes","Funding by the DFG collaborative research center SFB 755\r\nNanoscale Photonic Imaging and the German Ministry of Education and\r\nResearch (Grant No. 05K10MGA) is gratefully acknowledged."],["dc.notes.intern","Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","2192-2853"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Low-dose three-dimensional hard x-ray imaging of bacterial cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","867"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","878"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Sprung, Michael"],["dc.date.accessioned","2017-09-07T11:43:44Z"],["dc.date.available","2017-09-07T11:43:44Z"],["dc.date.issued","2015"],["dc.description.abstract","A compound optical system for coherent focusing and imaging at the nanoscale is reported, realised by high-gain fixed-curvature elliptical mirrors in combination with X-ray waveguide optics or different cleaning apertures. The key optical concepts are illustrated, as implemented at the Gottingen Instrument for Nano-Imaging with X-rays (GINIX), installed at the P10 coherence beamline of the PETRA III storage ring at DESY, Hamburg, and examples for typical applications in biological imaging are given. Characteristic beam configurations with the recently achieved values are also described, meeting the different requirements of the applications, such as spot size, coherence or bandwidth. The emphasis of this work is on the different beam shaping, filtering and characterization methods."],["dc.identifier.doi","10.1107/S1600577515007742"],["dc.identifier.gro","3141875"],["dc.identifier.isi","000357407900001"],["dc.identifier.pmid","26134789"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2045"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 755: Nanoscale Photonic Imaging"],["dc.relation.eissn","1600-5775"],["dc.relation.issn","0909-0495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Compound focusing mirror and X-ray waveguide optics for coherent imaging and nano-diffraction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","490"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","497"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-11-05T15:05:25Z"],["dc.date.available","2020-11-05T15:05:25Z"],["dc.date.issued","2013"],["dc.description.abstract","In the past decade Kirkpatrick-Baez (KB) mirrors have been established as powerful focusing systems in hard X-ray microscopy applications. Here a ptychographic characterization of the KB focus in the dedicated nano-imaging setup GINIX (Gottingen Instrument for Nano-Imaging with X-rays) at the P10 coherence beamline of the PETRA III synchrotron at HASLYLAB/DESY, Germany, is reported. More specifically, it is shown how aberrations in the KB beam, caused by imperfections in the height profile of the focusing mirrors, can be eliminated using a pinhole as a spatial filter near the focal plane. A combination of different pinhole sizes and illumination conditions of the KB setup makes the prepared optical setup well suited not only for high-resolution ptychographic coherent X-ray diffractive imaging but also for moderate-resolution/large-field-of-view propagation imaging in the divergent KB beam."],["dc.identifier.doi","10.1107/S0909049513005372"],["dc.identifier.gro","3142357"],["dc.identifier.isi","000317604800013"],["dc.identifier.pmid","23592629"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68466"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-352.6"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0909-0495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","Versatility of a hard X-ray Kirkpatrick–Baez focus characterized by ptychography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","051110"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Applied Physics Letters"],["dc.bibliographiccitation.volume","91"],["dc.contributor.author","Ollinger, C."],["dc.contributor.author","Fuhse, Christian"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Tucoulou, Remi"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:49:27Z"],["dc.date.available","2017-09-07T11:49:27Z"],["dc.date.issued","2007"],["dc.description.abstract","The present work focuses on the question of localizing single object by hard x-ray phase contrast projection imaging. The authors present a setup where an x-ray channel waveguide defines a \"quasi-point source\" used to illuminate and image an object in a highly coherent cone beam. Knife edge fluorescence scans revealed a beam diameter of 75 nm at a distance of 30 mu m behind the guide. The recorded image corresponds to an in-line hologram of the object which can be reconstructed numerically. Object translations and associated shifts in the hologram allow for the 10 nm localization accuracy. (c) 2007 American Institute of Physics."],["dc.identifier.doi","10.1063/1.2757601"],["dc.identifier.gro","3143465"],["dc.identifier.isi","000248595800010"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/982"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0003-6951"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.title","Object localization with 10 nm accuracy by x-ray phase contrast projection imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","529"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.lastpage","534"],["dc.bibliographiccitation.volume","107"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Thibault, Pierre"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Beerlink, André"],["dc.contributor.author","Kewish, Cameron M."],["dc.contributor.author","Dierolf, Martin"],["dc.contributor.author","Pfeiffer, Franz"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:46:10Z"],["dc.date.available","2017-09-07T11:46:10Z"],["dc.date.issued","2010"],["dc.description.abstract","Recent advances in coherent x-ray diffractive imaging have paved the way to reliable and quantitative imaging of noncompact specimens at the nanometer scale. Introduced a year ago, an advanced implementation of ptychographic coherent diffractive imaging has removed much of the previous limitations regarding sample preparation and illumination conditions. Here, we apply this recent approach toward structure determination at the nanoscale to biological microscopy. We show that the projected electron density of unstained and unsliced freeze-dried cells of the bacterium Deinococcus radiodurans can be derived from the reconstructed phase in a straightforward and reproducible way, with quantified and small errors. Thus, the approach may contribute in the future to the understanding of the highly disputed nucleoid structure of bacterial cells. In the present study, the estimated resolution for the cells was 85 nm (half-period length), whereas 50-nm resolution was demonstrated for lithographic test structures. With respect to the diameter of the pinhole used to illuminate the samples, a superresolution of about 15 was achieved for the cells and 30 for the test structures, respectively. These values should be assessed in view of the low dose applied on the order of similar or equal to 1.3 . 10(5) Gy, and were shown to scale with photon fluence."],["dc.identifier.doi","10.1073/pnas.0905846107"],["dc.identifier.fs","540456"],["dc.identifier.gro","3142982"],["dc.identifier.isi","000273559300004"],["dc.identifier.pmid","20018650"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7505"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/445"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0027-8424"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.title","Quantitative biological imaging by ptychographic x-ray diffraction microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]