Salditt, Tim

[["dc.bibliographiccitation.firstpage","561"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Structural Biology"],["dc.bibliographiccitation.lastpage","568"],["dc.bibliographiccitation.volume","192"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Cloetens, Peter"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:54:52Z"],["dc.date.available","2017-09-07T11:54:52Z"],["dc.date.issued","2015"],["dc.description.abstract","We have used X-ray phase contrast tomography to resolve the structure of uncut, entire myelinated optic, saphenous and sciatic mouse nerves. Intrinsic electron density contrast suffices to identify axonal structures. Specific myelin labeling by an osmium tetroxide stain enables distinction between axon and surrounding myelin sheath. Utilization of spherical wave illumination enables zooming capabilities which enable imaging of entire sciatic intemodes as well as identification of sub-structures such as nodes of Ranvier and Schmidt-Lanterman incisures. (C) 2015 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.jsb.2015.11.001"],["dc.identifier.gro","3141782"],["dc.identifier.isi","000365458400028"],["dc.identifier.pmid","26546551"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1013"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1095-8657"],["dc.relation.issn","1047-8477"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Myelinated mouse nerves studied by X-ray phase contrast zoom tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","012001"],["dc.bibliographiccitation.journal","Journal of Physics. Conference Series"],["dc.bibliographiccitation.volume","849"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Ruhwedel, Torben"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Pacureanu, A."],["dc.contributor.author","Cloetens, Peter"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-03-11T09:13:55Z"],["dc.date.available","2020-03-11T09:13:55Z"],["dc.date.issued","2017"],["dc.description.abstract","We present propagation-based phase-contrast tomography of mouse sciatic nerves stained with osmium, leading to an enhanced contrast in the myelin sheath around the axons, in order to visualize the threedimensional (3D) structure of the nerve. We compare different experimental parameters and show that contrast and resolution are high enough to identify single axons in the nerve, including characteristic functional structures such as Schmidt-Lanterman incisures."],["dc.identifier.doi","10.1088/1742-6596/849/1/012001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63293"],["dc.language.iso","en"],["dc.relation.issn","1742-6588"],["dc.relation.issn","1742-6596"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 3.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Phase-contrast tomography of sciatic nerves: image quality and experimental parameters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Quade, Felix"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Khounsary, Ali M."],["dc.contributor.editor","Dorssen, Gert E. van"],["dc.date.accessioned","2017-09-07T11:54:05Z"],["dc.date.available","2017-09-07T11:54:05Z"],["dc.date.issued","2016"],["dc.description.abstract","Due to the large penetration depth and small wavelength hard x-rays offer a unique potential for 3D biomedical and biological imaging, combining capabilities of high resolution and large sample volume. However, in classical absorption-based computed tomography, soft tissue only shows a weak contrast, limiting the actual resolution. With the advent of phase-contrast methods, the much stronger phase shift induced by the sample can now be exploited. For high resolution, free space propagation behind the sample is particularly well suited to make the phase shift visible. Contrast formation is based on the self-interference of the transmitted beam, resulting in object-induced intensity modulations in the detector plane. As this method requires a sufficiently high degree of spatial coherence, it was since long perceived as a synchrotron-based imaging technique. In this contribution we show that by combination of high brightness liquid-metal jet microfocus sources and suitable sample preparation techniques, as well as optimized geometry, detection and phase retrieval, excellent three-dimensional image quality can be obtained, revealing the anatomy of a cobweb spider in high detail. This opens up new opportunities for 3D virtual histology of small organisms. Importantly, the image quality is finally augmented to a level accessible to automatic 3D segmentation."],["dc.identifier.doi","10.1117/12.2246460"],["dc.identifier.gro","3145109"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2809"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","SPIE"],["dc.publisher.place","Bellingham, Washington"],["dc.relation.conference","Advances in Laboratory-Based X-Ray Sources, Optics, and Applications"],["dc.relation.eventend","2016-08-31"],["dc.relation.eventlocation","San Diego, Calif."],["dc.relation.eventstart","2016-08-30"],["dc.relation.ispartof","Advances in Laboratory-Based X-Ray Sources, Optics, and Applications V"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Laboratory-based x-ray phase-contrast tomography enables 3D virtual histology"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2220"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","2235"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:48:18Z"],["dc.date.available","2017-09-07T11:48:18Z"],["dc.date.issued","2013"],["dc.description.abstract","We have implemented a deterministic method for solving the phase problem in hard x-ray in-line holography which overcomes the twin image problem. The phase distribution in the detector plane is retrieved by using two images with slightly different Fresnel numbers. We then use measured intensities and reconstructed phases in the detection plane to compute the exit wave in the sample plane. No further a priori information like a limited support or the assumption of pure phase objects is necessary so that it can be used for a wide range of complex samples. Using a nano-focused hard x-ray beam half period resolutions better than 30 nm are achieved. (C) 2013 Optical Society of America"],["dc.identifier.doi","10.1364/OE.21.002220"],["dc.identifier.gro","3142400"],["dc.identifier.isi","000315989500086"],["dc.identifier.pmid","23389203"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/7863"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.title","Transport of intensity phase reconstruction to solve the twin image problem in holographic x-ray imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","143"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","155"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","dal Monego, Simeone"],["dc.contributor.author","Larsson, Emanuel"],["dc.contributor.author","Mohammadi, Sara"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Garrovo, Chiara"],["dc.contributor.author","Biffi, Stefania"],["dc.contributor.author","Lorenzon, Andrea"],["dc.contributor.author","Markus, Andrea"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Accardo, Agostino"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Tromba, Giuliana"],["dc.date.accessioned","2017-09-07T11:44:46Z"],["dc.date.available","2017-09-07T11:44:46Z"],["dc.date.issued","2015"],["dc.description.abstract","Functionalized computed tomography (CT) in combination with labelled cells is virtually non-existent due to the limited sensitivity of X-ray-absorption-based imaging, but would be highly desirable to realise cell tracking studies in entire organisms. In this study we applied in-line free propagation X-ray phase-contrast CT (XPCT) in an allergic asthma mouse model to assess structural changes as well as the biodistribution of barium-labelled macrophages in lung tissue. Alveolar macrophages that were barium-sulfate-loaded and fluorescent-labelled were instilled intratracheally into asthmatic and control mice. Mice were sacrificed after 24 h, lungs were kept in situ, inflated with air and scanned utilizing XPCT at the SYRMEP beamline (Elettra Synchrotron Light Source, Italy). Single-distance phase retrieval was used to generate data sets with ten times greater contrast-to-noise ratio than absorption-based CT (in our setup), thus allowing to depict and quantify structural hallmarks of asthmatic lungs such as reduced air volume, obstruction of airways and increased soft-tissue content. Furthermore, we found a higher concentration as well as a specific accumulation of the barium-labelled macrophages in asthmatic lung tissue. It is believe that XPCT will be beneficial in preclinical asthma research for both the assessment of therapeutic response as well as the analysis of the role of the recruitment of macrophages to inflammatory sites."],["dc.identifier.doi","10.1107/S1600577514021730"],["dc.identifier.fs","608140"],["dc.identifier.gro","3141991"],["dc.identifier.isi","000346850200022"],["dc.identifier.pmid","25537601"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11558"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3334"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/230739/EU//P3AGI"],["dc.relation.eissn","1600-5775"],["dc.relation.issn","0909-0495"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Functionalized synchrotron in-line phase-contrast computed tomography: a novel approach for simultaneous quantification of structural alterations and localization of barium-labelled alveolar macrophages within mouse lung samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","035007"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","AIP Advances"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Toepperwien, Mareike"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:54:36Z"],["dc.date.available","2017-09-07T11:54:36Z"],["dc.date.issued","2016"],["dc.description.abstract","We have performed high-resolution phase-contrast tomography on whole mice with a laboratory setup. Enabled by a high-brilliance liquid- metal- jet source, we show the feasibility of propagation-based phase contrast in local tomography even in the presence of strongly absorbing surrounding tissue as it is the case in small animal imaging of the lung. We demonstrate the technique by reconstructions of the mouse lung for two different fields of view, covering the whole organ, and a zoom to the local finer structure of terminal airways and alveoli. With a resolution of a few micrometers and the wide availability of the technique, studies of larger biological samples at the cellular level become possible. (c) 2016 Author(s)."],["dc.identifier.doi","10.1063/1.4943898"],["dc.identifier.gro","3141718"],["dc.identifier.isi","000373684200008"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13246"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/302"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: BMBF-Verbundforschung; [SFB 755]"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2158-3226"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Propagation-based phase-contrast tomography for high-resolution lung imaging with laboratory sources"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","033847"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Physical Review A"],["dc.bibliographiccitation.volume","89"],["dc.contributor.author","Ruhlandt, Aike"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:46:24Z"],["dc.date.available","2017-09-07T11:46:24Z"],["dc.date.issued","2014"],["dc.description.abstract","We present a solution to the phase problem in near-field x-ray (propagation) imaging. The three-dimensional complex-valued index of refraction is reconstructed from a set of projections recorded in the near-field (Fresnel) setting at a single detector distance. The solution is found by an iterative algorithm based only on the measured data and the three-dimensional tomographic (Helgason-Ludwig) consistency constraint without the need for further a priori knowledge or other restrictive assumptions."],["dc.identifier.doi","10.1103/PhysRevA.89.033847"],["dc.identifier.gro","3142164"],["dc.identifier.isi","000333410700008"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5244"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1094-1622"],["dc.relation.issn","1050-2947"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Three-dimensional phase retrieval in propagation-based phase-contrast imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","159901"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Applied Physics Letters"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Hernandez, Victor H."],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2018-11-07T09:18:43Z"],["dc.date.available","2018-11-07T09:18:43Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1063/1.4821922"],["dc.identifier.isi","000325779700099"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28467"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1077-3118"],["dc.relation.issn","0003-6951"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Phase contrast tomography of the mouse cochlea at microfocus x-ray sources"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","464"],["dc.bibliographiccitation.journal","Journal of Applied Crystallography"],["dc.bibliographiccitation.lastpage","476"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:44:29Z"],["dc.date.available","2017-09-07T11:44:29Z"],["dc.date.issued","2015"],["dc.description.abstract","Quantitative waveguide-based X-ray phase contrast imaging has been carried out on the level of single, unstained, unsliced and freeze-dried bacterial cells of Bacillus thuringiensis and Bacillus subtilis using hard X-rays of 7.9keV photon energy. The cells have been prepared in the metabolically dormant state of an endospore. The quantitative phase maps obtained by iterative phase retrieval using a modified hybrid input-output algorithm allow for mass and mass density determinations on the level of single individual endospores but include also large field of view investigations. Additionally, a direct reconstruction based on the contrast transfer function is investigated, and the two approaches are compared. Depending on the field of view and method, a resolution down to 65nm was achieved at a maximum applied dose of below 5 x 10(5)Gy. Masses in the range of about approximate to 110-190(20)fg for isolated endospores have been obtained."],["dc.identifier.doi","10.1107/S1600576715003593"],["dc.identifier.fs","615792"],["dc.identifier.gro","3141933"],["dc.identifier.isi","000352229100017"],["dc.identifier.pmid","25844079"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13672"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2691"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1600-5767"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","Quantitative X-ray phase contrast waveguide imaging of bacterial endospores"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","083703"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Applied Physics Letters"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Hernandez, Victor H."],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:47:37Z"],["dc.date.available","2017-09-07T11:47:37Z"],["dc.date.issued","2013"],["dc.description.abstract","We present phase contrast x-ray tomography of functional soft tissue within the bony cochlear capsule of mice, carried out at laboratory microfocus sources with well-matched source, detector, geometry, and reconstruction algorithms at spatial resolutions down to 2 mu m. Contrast, data quality and resolution enable the visualization of thin membranes and nerve fibers as well as automated segmentation of surrounding bone. By complementing synchrotron radiation imaging techniques, a broad range of biomedical applications becomes possible as demonstrated for optogenetic cochlear implant research. (C) 2013 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License."],["dc.identifier.doi","10.1063/1.4818737"],["dc.identifier.gro","3142304"],["dc.identifier.isi","000323788100094"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/6798"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0003-6951"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","Phase contrast tomography of the mouse cochlea at microfocus x-ray sources"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]