Salditt, Tim

[["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Müller, Kristin"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Stock, Stuart R."],["dc.contributor.editor","Müller, Bert"],["dc.contributor.editor","Wang, Ge"],["dc.date.accessioned","2017-09-07T11:54:06Z"],["dc.date.available","2017-09-07T11:54:06Z"],["dc.date.issued","2016"],["dc.description.abstract","Assessing the three-dimensional architecture of neuronal tissues with sub-cellular resolution presents a significant analytical challenge. Overcoming the limitations associated with serial slicing, phase-contrast x-ray tomography has the potential to contribute to this goal. Even compact laboratory CT at an optimized liquid-metal jet micro- focus source combined with suitable phase-retrieval algorithms and preparation protocols can yield renderings with single cell sensitivity in millimeter sized brain areas of mouse. Here, we show the capabilities of the setup by imaging a Golgi-Cox impregnated mouse brain. Towards higher resolution we extend these studies at our recently upgraded waveguide-based cone-beam holo-tomography instrument GINIX at DESY. This setup allows high resolution recordings with adjustable field of view and resolution, down to the voxel sizes in the range of a few ten nanometers. The recent results make us confident that important issues of neuronal connectivity can be addressed by these methods, and that 3D (virtual) histology with nanoscale resolution will become an attractive modality for neuroscience research."],["dc.identifier.doi","10.1117/12.2238496"],["dc.identifier.gro","3145108"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2808"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","SPIE-Intl Soc Optical Eng"],["dc.publisher.place","Bellingham, Washington"],["dc.relation.conference","Developments in X-Ray Tomography X"],["dc.relation.eventend","2016-08-31"],["dc.relation.eventlocation","San Diego, Calif."],["dc.relation.eventstart","2016-08-29"],["dc.relation.isbn","978-1-5106-0325-7"],["dc.relation.ispartof","Developments in X-Ray Tomography X"],["dc.relation.issn","0277-786X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Phase-contrast tomography of neuronal tissues: from laboratory- to high resolution synchrotron CT"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","9212-26"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Lingor, Paul"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Stock, Stuart R."],["dc.date.accessioned","2017-09-07T11:54:06Z"],["dc.date.available","2017-09-07T11:54:06Z"],["dc.date.issued","2014"],["dc.description.abstract","We use propagation based hard x-ray phase contrast tomography to explore the three dimensional structure of neuronal tissues from the organ down to sub-cellular level, based on combinations of synchrotron radiation and laboratory sources. To this end a laboratory based microfocus tomography setup has been built in which the geometry was optimized for phase contrast imaging and tomography. By utilizing phase retrieval algorithms, quantitative reconstructions can be obtained that enable automatic renderings without edge artifacts. A high brightness liquid metal microfocus x-ray source in combination with a high resolution detector yielding a resolution down to 1.5 μm. To extend the method to nanoscale resolution we use a divergent x-ray waveguide beam geometry at the synchrotron. Thus, the magnification can be easily tuned by placing the sample at different defocus distances. Due to the small Fresnel numbers in this geometry the measured images are of holographic nature which poses a challenge in phase retrieval."],["dc.identifier.doi","10.1117/12.2060390"],["dc.identifier.gro","3145112"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2812"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","SPIE"],["dc.publisher.place","Bellingham, Washington"],["dc.relation.conference","9th Conference Developments in X-Ray Tomography"],["dc.relation.eventend","2014-08-20"],["dc.relation.eventlocation","San Diego, Calif."],["dc.relation.eventstart","2014-08-18"],["dc.relation.ispartof","Developments in X-Ray Tomography IX"],["dc.relation.issn","0277-786X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","X-ray phase contrast tomography from whole organ down to single cells"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]