Salditt, Tim

[["dc.bibliographiccitation.artnumber","111130N"],["dc.bibliographiccitation.firstpage","21"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Ruhwedel, Torben"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Müller-Myhsok, Bertram"],["dc.contributor.editor","Wang, Geng"],["dc.date.accessioned","2020-03-10T15:20:27Z"],["dc.date.available","2020-03-10T15:20:27Z"],["dc.date.issued","2019"],["dc.description.abstract","In the present work, we evaluate and compare the contrast and resolution obtained on different neuronal tissues with propagation-based x-ray phase contrast computed tomography (PB-CT). At our laboratory-based liquid metal-jet setup, we obtain overview datasets at sub-micron resolution of mm3 -sized volumes. In order to evaluate these parameters down to the sub-cellular level, we utilize the synchrotron endstation GINIX at P10, DESY. At this dedicated endstation1 developed and operated by our group, we utilize x-ray waveguide optics for highresolution cone-beam scans at strong geometrical magnification M. Exploiting this multi-scale approach, we investigate the image quality of cerebellum tissue treated by different heavy-metal stains. In addition, we study the electron density contrast in unstained tissues. Different embedding media are utilized depending on the stain, which also significantly affects contrast and image quality. With this work, we want to contribute to an optimized sample preparation to study the neuronal architecture of the brain tissue in greater detail in three dimensions (3d). © (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only."],["dc.identifier.doi","10.1117/12.2528432"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63286"],["dc.language.iso","en"],["dc.relation.eventend","2019-08-15"],["dc.relation.eventlocation","San Diego, California, United States"],["dc.relation.eventstart","2019-08-11"],["dc.relation.isbn","978-1-5106-2919-6"],["dc.relation.isbn","978-1-5106-2920-2"],["dc.relation.ispartof","Proc. SPIE 11113"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","biomedical tomography"],["dc.title","Evaluation of different heavy-metal stains and embedding media for phase contrast tomography of neuronal tissue"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4"],["dc.contributor.author","Nicolas, J. D."],["dc.contributor.author","Markus, Marietta Andrea"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Wang, Geng"],["dc.contributor.editor","Müller-Myhsok, Bertram"],["dc.date.accessioned","2020-06-26T10:11:47Z"],["dc.date.available","2020-06-26T10:11:47Z"],["dc.date.issued","2017"],["dc.description.abstract","In this work we present x-ray phase-contrast tomography of heart tissue from mouse, combining computed tomography (CT) scans with laboratory and synchrotron radiation. The work serves as a proof-of-concept that the cyto-architecture and in particular the myofibril orientation can be assessed in three dimensions (3D) by phase-contrast CT. We demonstrate the synergistic use of laboratory μ -CT and of the high resolution synchrotron setup based on waveguide optics. Details on preparation, instrumentation and analysis are given, as a state of the art reference for heart tissue tomography, and as a starting point for further progress."],["dc.identifier.doi","10.1117/12.2276648"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66750"],["dc.language.iso","en"],["dc.notes.preprint","yes"],["dc.relation.eventend","2017-09-25"],["dc.relation.eventlocation","San Diego"],["dc.relation.eventstart","2017-09-25"],["dc.relation.isbn","9781510612396"],["dc.relation.isbn","9781510612402"],["dc.relation.iserratumof","yes"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Nanoscale holographic tomography of heart tissue with x-ray waveguide optics"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]