Meyer, Daniel

[["dc.bibliographiccitation.firstpage","9104-9115"],["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Nanoscale"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Telele, Saba"],["dc.contributor.author","Zelená, Anna"],["dc.contributor.author","Gillen, Alice J."],["dc.contributor.author","Antonucci, Alessandra"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Mann, Florian A."],["dc.contributor.author","Erpenbeck, Luise"],["dc.contributor.author","Boghossian, Ardemis A."],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-06-26T11:13:39Z"],["dc.date.available","2020-06-26T11:13:39Z"],["dc.date.issued","2020"],["dc.description.abstract","Cells can take up nanoscale materials, which has important implications for understanding cellular functions, biocompatibility as well as biomedical applications. Controlled uptake, transport and triggered release of nanoscale cargo is one of the great challenges in biomedical applications of nanomaterials. Here, we study how human immune cells (neutrophilic granulocytes, neutrophils) take up nanomaterials and program them to release this cargo after a certain time period. For this purpose, we let neutrophils phagocytose DNA-functionalized single-walled carbon nanotubes (SWCNTs) in vitro that fluoresce in the near infrared (980 nm) and serve as sensors for small molecules. Cells still migrate, follow chemical gradients and respond to inflammatory signals after uptake of the cargo. To program release, we make use of neutrophil extracellular trap formation (NETosis), a novel cell death mechanism that leads to chromatin swelling, subsequent rupture of the cellular membrane and release of the cell's whole content. By using the process of NETosis, we can program the time point of cargo release via the initial concentration of stimuli such as phorbol 12-myristate-13-acetate (PMA) or lipopolysaccharide (LPS). At intermediate stimulation, cells continue to migrate, follow gradients and surface cues for around 30 minutes and up to several hundred micrometers until they stop and release the SWCNTs. The transported and released SWCNT sensors are still functional as shown by subsequent detection of the neurotransmitter dopamine and reactive oxygen species (H2O2). In summary, we hijack a biological process (NETosis) and demonstrate how neutrophils transport and release functional nanomaterials."],["dc.identifier.doi","10.1039/d0nr00864h"],["dc.identifier.pmid","32286598"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66755"],["dc.language.iso","en"],["dc.relation.eissn","2040-3372"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.rights","CC BY 3.0"],["dc.subject.gro","cellular biophysics"],["dc.title","Transport and programmed release of nanoscale cargo from cells by using NETosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Chizhik, Alexey"],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Kuhlemann, llyas"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Vuong, Loan"],["dc.contributor.author","Preiß, Helen"],["dc.contributor.author","Herrmann, Niklas"],["dc.contributor.author","Mann, Florian A."],["dc.contributor.author","Lv, Zhiyi"],["dc.contributor.author","Oswald, Tabea A."],["dc.contributor.author","Spreinat, Alexander"],["dc.contributor.author","Erpenbeck, Luise"],["dc.contributor.author","Großhans, Jörg"],["dc.contributor.author","Karius, Volker"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Pablo Giraldo, Juan"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-11-05T15:08:10Z"],["dc.date.available","2020-11-05T15:08:10Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2020"],["dc.identifier.doi","10.1038/s41467-020-15299-5"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17352"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68478"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-352.7"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","2041-1723"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Exfoliated near infrared fluorescent silicate nanosheets for (bio)photonics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4017"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","4027"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Hagemann, Annika"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2018-11-07T10:25:24Z"],["dc.date.available","2018-11-07T10:25:24Z"],["dc.date.issued","2017"],["dc.description.abstract","Fluorescent nanosensors are powerful tools for basic research and bioanalytical applications. Individual nanosensors are able to detect single molecules, while ensembles of nanosensors can be used to measure the bulk concentration of an analyte. Collective imaging of multiple nanosensors could provide both spatial and temporal chemical information from the nano- to the microscale. This type of chemical imaging with nanosensors would be very attractive to study processes such as chemical signaling between cells (e.g., neurons). So far, it is not understood what processes are resolvable (concentration, time, space) and how optimal sensors should be designed. Here, we develop a theoretical framework to simulate the fluorescence image of arrays of nanosensors in response to a concentration gradient. For that purpose, binding and unbinding of the analyte is simulated for each single nanosensor by using a Monte Carlo simulation and varying rate constants (k(on), k(off)). Multiple nanosensors are arranged on a surface and exposed to a concentration pattern c(A)(x,y,t) of an analyte. We account for the resolution limit of light microscopy (Abbe limit) and the acquisition speed and resolution of optical setups and determine the resulting response images Delta I(x,y,t). Consequently, we introduce terms for the spatial and temporal resolution and simulate phase diagrams for different rate constants that allow us to predict how a sensor should be designed to provide a desired spatial and temporal resolution. Our results show, for example, that imaging of neurotransmitter release requires rate constants of k(on) = 10(6) M-1 s(-1) and k(off) = 10(2) s(-1) in many scenarios, which corresponds to high dissociation constants of K-d > 100 mu M. This work predicts if a given fluorescent nanosensor array (rate constants, size, shape, geometry, density) is able to resolve fast concentration changes such as neurotransmitter release from cells. Additionally, we provide rational design principles to engineer nanosensors for chemical imaging."],["dc.identifier.doi","10.1021/acsnano.7b00569"],["dc.identifier.isi","000400233200062"],["dc.identifier.pmid","28379687"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42854"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.title","Kinetic Requirements for Spatiotemporal Chemical Imaging with Fluorescent Nanosensors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","3767"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Rocca, Francesco"],["dc.contributor.author","Günay, Gökhan"],["dc.contributor.author","Kwaczala-Tessmann, Anja"],["dc.contributor.author","Grandke, Julia"],["dc.contributor.author","Senger-Sander, Susanne"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Schön, Michael P."],["dc.contributor.author","Erpenbeck, Luise"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2019-07-09T11:45:55Z"],["dc.date.available","2019-07-09T11:45:55Z"],["dc.date.issued","2018"],["dc.description.abstract","Neutrophilic granulocytes are able to release their own DNA as neutrophil extracellular traps (NETs) to capture and eliminate pathogens. DNA expulsion (NETosis) has also been documented for other cells and organisms, thus highlighting the evolutionary conservation of this process. Moreover, dysregulated NETosis has been implicated in many diseases, including cancer and inflammatory disorders. During NETosis, neutrophils undergo dynamic and dramatic alterations of their cellular as well as sub-cellular morphology whose biophysical basis is poorly understood. Here we investigate NETosis in real-time on the single-cell level using fluorescence and atomic force microscopy. Our results show that NETosis is highly organized into three distinct phases with a clear point of no return defined by chromatin status. Entropic chromatin swelling is the major physical driving force that causes cell morphology changes and the rupture of both nuclear envelope and plasma membrane. Through its material properties, chromatin thus directly orchestrates this complex biological process."],["dc.identifier.doi","10.1038/s41467-018-06263-5"],["dc.identifier.pmid","30218080"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15346"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59338"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Chromatin swelling drives neutrophil extracellular trap release"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1521"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Sensors (Basel, Switzerland)"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Mann, Florian A."],["dc.contributor.author","Herrmann, Niklas"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2019-07-09T11:43:35Z"],["dc.date.available","2019-07-09T11:43:35Z"],["dc.date.issued","2017-06-28"],["dc.description.abstract","Detection of neurotransmitters is an analytical challenge and essential to understand neuronal networks in the brain and associated diseases. However, most methods do not provide sufficient spatial, temporal, or chemical resolution. Near-infrared (NIR) fluorescent single-walled carbon nanotubes (SWCNTs) have been used as building blocks for sensors/probes that detect catecholamine neurotransmitters, including dopamine. This approach provides a high spatial and temporal resolution, but it is not understood if these sensors are able to distinguish dopamine from similar catecholamine neurotransmitters, such as epinephrine or norepinephrine. In this work, the organic phase (DNA sequence) around SWCNTs was varied to create sensors with different selectivity and sensitivity for catecholamine neurotransmitters. Most DNA-functionalized SWCNTs responded to catecholamine neurotransmitters, but both dissociation constants (Kd) and limits of detection were highly dependent on functionalization (sequence). Kd values span a range of 2.3 nM (SWCNT-(GC)15 + norepinephrine) to 9.4 μM (SWCNT-(AT)15 + dopamine) and limits of detection are mostly in the single-digit nM regime. Additionally, sensors of different SWCNT chirality show different fluorescence increases. Moreover, certain sensors (e.g., SWCNT-(GT)10) distinguish between different catecholamines, such as dopamine and norepinephrine at low concentrations (50 nM). These results show that SWCNTs functionalized with certain DNA sequences are able to discriminate between catecholamine neurotransmitters or to detect them in the presence of interfering substances of similar structure. Such sensors will be useful to measure and study neurotransmitter signaling in complex biological settings."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.3390/s17071521"],["dc.identifier.pmid","28657584"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14585"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58922"],["dc.language.iso","en"],["dc.notes.intern","DeepGreen Import"],["dc.publisher","MDPI"],["dc.relation.eissn","1424-8220"],["dc.relation.issn","1424-8220"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.rights.access","openAccess"],["dc.subject.ddc","540"],["dc.title","Tuning Selectivity of Fluorescent Carbon Nanotube-Based Neurotransmitter Sensors."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2320"],["dc.bibliographiccitation.journal","Frontiers in Immunology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Gruhn, Antonia Luise"],["dc.contributor.author","Kudryasheva, Galina"],["dc.contributor.author","Günay, Gökhan"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Busse, Julia"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Erpenbeck, Luise"],["dc.contributor.author","Schön, Michael P."],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-11-18T14:37:03Z"],["dc.date.available","2020-11-18T14:37:03Z"],["dc.date.issued","2019"],["dc.description.abstract","Neutrophils are the most abundant type of white blood cells. Upon stimulation, they are able to decondense and release their chromatin as neutrophil extracellular traps (NETs). This process (NETosis) is part of immune defense mechanisms but also plays an important role in many chronic and inflammatory diseases such as atherosclerosis, rheumatoid arthritis, diabetes, and cancer. For this reason, much effort has been invested into understanding biochemical signaling pathways in NETosis. However, the impact of the mechanical micro-environment and adhesion on NETosis is not well-understood. Here, we studied how adhesion and especially substrate elasticity affect NETosis. We employed polyacrylamide (PAA) gels with distinctly defined elasticities (Young's modulus E) within the physiologically relevant range from 1 to 128 kPa and coated the gels with integrin ligands (collagen I, fibrinogen). Neutrophils were cultured on these substrates and stimulated with potent inducers of NETosis: phorbol 12-myristate 13-acetate (PMA) and lipopolysaccharide (LPS). Interestingly, PMA-induced NETosis was neither affected by substrate elasticity nor by different integrin ligands. In contrast, for LPS stimulation, NETosis rates increased with increasing substrate elasticity (E > 20 kPa). LPS-induced NETosis increased with increasing cell contact area, while PMA-induced NETosis did not require adhesion at all. Furthermore, inhibition of phosphatidylinositide 3 kinase (PI3K), which is involved in adhesion signaling, completely abolished LPS-induced NETosis but only slightly decreased PMA-induced NETosis. In summary, we show that LPS-induced NETosis depends on adhesion and substrate elasticity while PMA-induced NETosis is completely independent of adhesion."],["dc.identifier.doi","10.3389/fimmu.2019.02320"],["dc.identifier.pmid","31632402"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16478"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68803"],["dc.language.iso","en"],["dc.notes.intern","DeepGreen Import"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-3224"],["dc.relation.issn","1664-3224"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Effect of Adhesion and Substrate Elasticity on Neutrophil Extracellular Trap Formation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]