Meyer, Daniel

[["dc.bibliographiccitation.firstpage","6604"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","6611"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Dinarvand, Meshkat"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Mann, Florian A"],["dc.contributor.author","Erpenbeck, Luise"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-11-18T14:37:07Z"],["dc.date.available","2020-11-18T14:37:07Z"],["dc.date.issued","2019"],["dc.description.abstract","Serotonin is an important neurotransmitter involved in various functions of the nervous, blood, and immune system. In general, detection of small biomolecules such as serotonin in real time with high spatial and temporal resolution remains challenging with conventional sensors and methods. In this work, we designed a near-infrared (nIR) fluorescent nanosensor (NIRSer) based on fluorescent single-walled carbon nanotubes (SWCNTs) to image the release of serotonin from human blood platelets in real time. The nanosensor consists of a nonbleaching SWCNT backbone, which is fluorescent in the beneficial nIR tissue transparency window (800-1700 nm) and a serotonin binding DNA aptamer. The fluorescence of the NIRSer sensor (995 nm emission wavelength for (6,5)-SWCNTs) increases in response to serotonin by a factor up to 1.8. It detects serotonin reversibly with a dissociation constant of 301 nM ± 138 nM and a dynamic linear range in the physiologically relevant region from 100 nM to 1 μM. As a proof of principle, we detected serotonin release patterns from activated platelets on the single-cell level. Imaging of the nanosensors around and under the platelets enabled us to locate hot spots of serotonin release and quantify the time delay (≈ 21-30 s) between stimulation and release in a population of platelets, highlighting the spatiotemporal resolution of this nanosensor approach. In summary, we report a nIR fluorescent nanosensor for the neurotransmitter serotonin and show its potential for imaging of chemical communication between cells."],["dc.identifier.doi","10.1021/acs.nanolett.9b02865"],["dc.identifier.pmid","31418577"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68804"],["dc.language.iso","en"],["dc.relation.eissn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.title","Near-Infrared Imaging of Serotonin Release from Cells with Fluorescent Nanosensors"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","jcs241075"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Cell Science"],["dc.bibliographiccitation.volume","133"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Kruss, Sebastian"],["dc.contributor.author","Erpenbeck, Luise"],["dc.date.accessioned","2020-11-18T14:36:58Z"],["dc.date.available","2020-11-18T14:36:58Z"],["dc.date.issued","2020"],["dc.description.abstract","Neutrophil extracellular traps (NETs) are one of the most intriguing discoveries in immunological research of the past few years. After their first description in 2004, the number of research articles on how NETs affect immunodefense, and also how they contribute to an ever-growing number of diseases, has skyrocketed. However, tempting as it may seem to plunge into pharmaceutical approaches to tamper with NET formation, our understanding of this complex process is still incomplete. Important concepts such as the context-dependent dual functions of NETs, in that they are both inflammatory and anti-inflammatory, or the major intra- and extracellular forces driving NET formation, are only emerging. In this Review, we summarize key aspects of our current understanding of NET formation (also termed NETosis), emphasize biophysical aspects and focus on three key principles - rearrangement and destabilization of the plasma membrane and the cytoskeleton, alterations and disassembly of the nuclear envelope, and chromatin decondensation as a driving force of intracellular reorganization."],["dc.identifier.doi","10.1242/jcs.241075"],["dc.identifier.pmid","32156720"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68802"],["dc.language.iso","en"],["dc.relation.eissn","1477-9137"],["dc.relation.issn","0021-9533"],["dc.title","The power from within - understanding the driving forces of neutrophil extracellular trap formation"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]