Meyer, Daniel

[["dc.bibliographiccitation.firstpage","6604"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","6611"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Dinarvand, Meshkat"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Mann, Florian A"],["dc.contributor.author","Erpenbeck, Luise"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-11-18T14:37:07Z"],["dc.date.available","2020-11-18T14:37:07Z"],["dc.date.issued","2019"],["dc.description.abstract","Serotonin is an important neurotransmitter involved in various functions of the nervous, blood, and immune system. In general, detection of small biomolecules such as serotonin in real time with high spatial and temporal resolution remains challenging with conventional sensors and methods. In this work, we designed a near-infrared (nIR) fluorescent nanosensor (NIRSer) based on fluorescent single-walled carbon nanotubes (SWCNTs) to image the release of serotonin from human blood platelets in real time. The nanosensor consists of a nonbleaching SWCNT backbone, which is fluorescent in the beneficial nIR tissue transparency window (800-1700 nm) and a serotonin binding DNA aptamer. The fluorescence of the NIRSer sensor (995 nm emission wavelength for (6,5)-SWCNTs) increases in response to serotonin by a factor up to 1.8. It detects serotonin reversibly with a dissociation constant of 301 nM ± 138 nM and a dynamic linear range in the physiologically relevant region from 100 nM to 1 μM. As a proof of principle, we detected serotonin release patterns from activated platelets on the single-cell level. Imaging of the nanosensors around and under the platelets enabled us to locate hot spots of serotonin release and quantify the time delay (≈ 21-30 s) between stimulation and release in a population of platelets, highlighting the spatiotemporal resolution of this nanosensor approach. In summary, we report a nIR fluorescent nanosensor for the neurotransmitter serotonin and show its potential for imaging of chemical communication between cells."],["dc.identifier.doi","10.1021/acs.nanolett.9b02865"],["dc.identifier.pmid","31418577"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68804"],["dc.language.iso","en"],["dc.relation.eissn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.title","Near-Infrared Imaging of Serotonin Release from Cells with Fluorescent Nanosensors"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","12241-12245"],["dc.bibliographiccitation.issue","47"],["dc.bibliographiccitation.journal","Chemistry: a European Journal"],["dc.bibliographiccitation.lastpage","12245"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Mann, Florian A"],["dc.contributor.author","Horlebein, Jan"],["dc.contributor.author","Meyer, Nils Frederik"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Thomas, Franziska"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-11-18T14:37:17Z"],["dc.date.available","2020-11-18T14:37:17Z"],["dc.date.issued","2018-08-22"],["dc.description.abstract","Specific functionalization of 1D nanomaterials such as near infrared (nIR) fluorescent single-walled carbon nanotubes (SWCNTs) is essential for colloidal stability and tailoring of their interactions with the environment. Here, we show that de novo designed alpha-helical coiled-coil peptide barrels (αHBs) with appropriate pores encapsulate and solubilize SWCNTs. In contrast, barrels without or with narrow pores showed a much smaller efficiency. Absorption/fluorescence spectroscopy and atomic force microscopy indicate that the SWCNTs are incorporated into the αHB's pore. The resulting hybrid SWCNT@αHBs display periodic surface coverage with a 40 nm pitch and remain fluorescent in the nIR. This approach presents a novel concept to encapsulate, discriminate and functionalize SWCNTs non-covalently with peptides and holds great promise for future applications in bioimaging or drug delivery."],["dc.identifier.doi","10.1002/chem.201800993"],["dc.identifier.pmid","29488660"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68806"],["dc.language.iso","en"],["dc.relation.eissn","1521-3765"],["dc.relation.issn","0947-6539"],["dc.title","Carbon Nanotubes Encapsulated in Coiled-Coil Peptide Barrels"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]