Meyer, Daniel

[["dc.bibliographiccitation.firstpage","6604"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","6611"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Dinarvand, Meshkat"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Mann, Florian A"],["dc.contributor.author","Erpenbeck, Luise"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-11-18T14:37:07Z"],["dc.date.available","2020-11-18T14:37:07Z"],["dc.date.issued","2019"],["dc.description.abstract","Serotonin is an important neurotransmitter involved in various functions of the nervous, blood, and immune system. In general, detection of small biomolecules such as serotonin in real time with high spatial and temporal resolution remains challenging with conventional sensors and methods. In this work, we designed a near-infrared (nIR) fluorescent nanosensor (NIRSer) based on fluorescent single-walled carbon nanotubes (SWCNTs) to image the release of serotonin from human blood platelets in real time. The nanosensor consists of a nonbleaching SWCNT backbone, which is fluorescent in the beneficial nIR tissue transparency window (800-1700 nm) and a serotonin binding DNA aptamer. The fluorescence of the NIRSer sensor (995 nm emission wavelength for (6,5)-SWCNTs) increases in response to serotonin by a factor up to 1.8. It detects serotonin reversibly with a dissociation constant of 301 nM ± 138 nM and a dynamic linear range in the physiologically relevant region from 100 nM to 1 μM. As a proof of principle, we detected serotonin release patterns from activated platelets on the single-cell level. Imaging of the nanosensors around and under the platelets enabled us to locate hot spots of serotonin release and quantify the time delay (≈ 21-30 s) between stimulation and release in a population of platelets, highlighting the spatiotemporal resolution of this nanosensor approach. In summary, we report a nIR fluorescent nanosensor for the neurotransmitter serotonin and show its potential for imaging of chemical communication between cells."],["dc.identifier.doi","10.1021/acs.nanolett.9b02865"],["dc.identifier.pmid","31418577"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68804"],["dc.language.iso","en"],["dc.relation.eissn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.title","Near-Infrared Imaging of Serotonin Release from Cells with Fluorescent Nanosensors"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","jcs241075"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Cell Science"],["dc.bibliographiccitation.volume","133"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Kruss, Sebastian"],["dc.contributor.author","Erpenbeck, Luise"],["dc.date.accessioned","2020-11-18T14:36:58Z"],["dc.date.available","2020-11-18T14:36:58Z"],["dc.date.issued","2020"],["dc.description.abstract","Neutrophil extracellular traps (NETs) are one of the most intriguing discoveries in immunological research of the past few years. After their first description in 2004, the number of research articles on how NETs affect immunodefense, and also how they contribute to an ever-growing number of diseases, has skyrocketed. However, tempting as it may seem to plunge into pharmaceutical approaches to tamper with NET formation, our understanding of this complex process is still incomplete. Important concepts such as the context-dependent dual functions of NETs, in that they are both inflammatory and anti-inflammatory, or the major intra- and extracellular forces driving NET formation, are only emerging. In this Review, we summarize key aspects of our current understanding of NET formation (also termed NETosis), emphasize biophysical aspects and focus on three key principles - rearrangement and destabilization of the plasma membrane and the cytoskeleton, alterations and disassembly of the nuclear envelope, and chromatin decondensation as a driving force of intracellular reorganization."],["dc.identifier.doi","10.1242/jcs.241075"],["dc.identifier.pmid","32156720"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68802"],["dc.language.iso","en"],["dc.relation.eissn","1477-9137"],["dc.relation.issn","0021-9533"],["dc.title","The power from within - understanding the driving forces of neutrophil extracellular trap formation"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","12241-12245"],["dc.bibliographiccitation.issue","47"],["dc.bibliographiccitation.journal","Chemistry: a European Journal"],["dc.bibliographiccitation.lastpage","12245"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Mann, Florian A"],["dc.contributor.author","Horlebein, Jan"],["dc.contributor.author","Meyer, Nils Frederik"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Thomas, Franziska"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-11-18T14:37:17Z"],["dc.date.available","2020-11-18T14:37:17Z"],["dc.date.issued","2018-08-22"],["dc.description.abstract","Specific functionalization of 1D nanomaterials such as near infrared (nIR) fluorescent single-walled carbon nanotubes (SWCNTs) is essential for colloidal stability and tailoring of their interactions with the environment. Here, we show that de novo designed alpha-helical coiled-coil peptide barrels (αHBs) with appropriate pores encapsulate and solubilize SWCNTs. In contrast, barrels without or with narrow pores showed a much smaller efficiency. Absorption/fluorescence spectroscopy and atomic force microscopy indicate that the SWCNTs are incorporated into the αHB's pore. The resulting hybrid SWCNT@αHBs display periodic surface coverage with a 40 nm pitch and remain fluorescent in the nIR. This approach presents a novel concept to encapsulate, discriminate and functionalize SWCNTs non-covalently with peptides and holds great promise for future applications in bioimaging or drug delivery."],["dc.identifier.doi","10.1002/chem.201800993"],["dc.identifier.pmid","29488660"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68806"],["dc.language.iso","en"],["dc.relation.eissn","1521-3765"],["dc.relation.issn","0947-6539"],["dc.title","Carbon Nanotubes Encapsulated in Coiled-Coil Peptide Barrels"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4017"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","4027"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Hagemann, Annika"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2018-11-07T10:25:24Z"],["dc.date.available","2018-11-07T10:25:24Z"],["dc.date.issued","2017"],["dc.description.abstract","Fluorescent nanosensors are powerful tools for basic research and bioanalytical applications. Individual nanosensors are able to detect single molecules, while ensembles of nanosensors can be used to measure the bulk concentration of an analyte. Collective imaging of multiple nanosensors could provide both spatial and temporal chemical information from the nano- to the microscale. This type of chemical imaging with nanosensors would be very attractive to study processes such as chemical signaling between cells (e.g., neurons). So far, it is not understood what processes are resolvable (concentration, time, space) and how optimal sensors should be designed. Here, we develop a theoretical framework to simulate the fluorescence image of arrays of nanosensors in response to a concentration gradient. For that purpose, binding and unbinding of the analyte is simulated for each single nanosensor by using a Monte Carlo simulation and varying rate constants (k(on), k(off)). Multiple nanosensors are arranged on a surface and exposed to a concentration pattern c(A)(x,y,t) of an analyte. We account for the resolution limit of light microscopy (Abbe limit) and the acquisition speed and resolution of optical setups and determine the resulting response images Delta I(x,y,t). Consequently, we introduce terms for the spatial and temporal resolution and simulate phase diagrams for different rate constants that allow us to predict how a sensor should be designed to provide a desired spatial and temporal resolution. Our results show, for example, that imaging of neurotransmitter release requires rate constants of k(on) = 10(6) M-1 s(-1) and k(off) = 10(2) s(-1) in many scenarios, which corresponds to high dissociation constants of K-d > 100 mu M. This work predicts if a given fluorescent nanosensor array (rate constants, size, shape, geometry, density) is able to resolve fast concentration changes such as neurotransmitter release from cells. Additionally, we provide rational design principles to engineer nanosensors for chemical imaging."],["dc.identifier.doi","10.1021/acsnano.7b00569"],["dc.identifier.isi","000400233200062"],["dc.identifier.pmid","28379687"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42854"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.title","Kinetic Requirements for Spatiotemporal Chemical Imaging with Fluorescent Nanosensors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4837"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","The Journal of Physical Chemistry. C, Nanomaterials and interfaces"],["dc.bibliographiccitation.lastpage","4847"],["dc.bibliographiccitation.volume","123"],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Mann, Florian A."],["dc.contributor.author","Chaturvedi, Parth"],["dc.contributor.author","Horlebein, Jan"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Vuković, Lela"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-11-18T14:37:11Z"],["dc.date.available","2020-11-18T14:37:11Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1021/acs.jpcc.8b11058"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68805"],["dc.relation.issn","1932-7447"],["dc.relation.issn","1932-7455"],["dc.title","Quantification of the Number of Adsorbed DNA Molecules on Single-Walled Carbon Nanotubes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]