Heidenreich, Doris

[["dc.bibliographiccitation.journal","PLoS currents"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Heidenreich, Doris"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2019-07-09T11:40:12Z"],["dc.date.available","2019-07-09T11:40:12Z"],["dc.date.issued","2013"],["dc.description.abstract","The emergence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in the eastern Mediterranean and imported cases to Europe has alerted public health authorities. Currently, detection of MERS-CoV in patient samples is done by real-time RT-PCR. Samples collected from suspected cases are sent to highly-equipped centralized laboratories for screening. A rapid point-of-care test is needed to allow more widespread mobile detection of the virus directly from patient material. In this study, we describe the development of a reverse transcription isothermal Recombinase Polymerase Amplification (RT-RPA) assay for the identification of MERS-CoV. A partial nucleocapsid gene RNA molecular standard of MERS-coronavirus was used to determine the assay sensitivity. The isothermal (42°C) MERS-CoV RT-RPA was as sensitive as real-time RT-PCR (10 RNA molecules), rapid (3-7 minutes) and mobile (using tubescanner weighing 1kg). The MERS-CoV RT-RPA showed cross-detection neither of any of the RNAs of several coronaviruses and respiratory viruses affecting humans nor of the human genome. The developed isothermal real-time RT-RPA is ideal for rapid mobile molecular MERS-CoV monitoring in acute patients and may also facilitate the search for the animal reservoir of MERS-CoV."],["dc.identifier.doi","10.1371/currents.outbreaks.62df1c7c75ffc96cd59034531e2e8364"],["dc.identifier.fs","601231"],["dc.identifier.pmid","24459611"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10752"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58113"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2157-3999"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Reverse transcription recombinase polymerase amplification assay for the detection of middle East respiratory syndrome coronavirus."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0129682"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Thaloengsok, Sasikanya"],["dc.contributor.author","Heidenreich, Doris"],["dc.contributor.author","Matangkasombut, Ponpan"],["dc.contributor.author","Manopwisedjaroen, Khajohnpong"],["dc.contributor.author","Sakuntabhai, Anavaj"],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2018-11-07T09:55:51Z"],["dc.date.available","2018-11-07T09:55:51Z"],["dc.date.issued","2015"],["dc.description.abstract","Background Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for molecular detection of the dengue virus (DENV). Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4. Methodology/Principal Findings Using two quantitative RNA molecular standards, the analytical sensitivity of a RT-RPA targeting the 3'non-translated region of DENV1-4 was found to range from 14 (DENV4) to 241 (DENV1-3) RNA molecules detected. The assay was specific and did not cross detect other Flaviviruses. The RT-RPA assay was tested in a mobile laboratory combining magneticbead based total nucleic acid extraction and a portable detection device in Kedougou (Senegal) and in Bangkok (Thailand). In Kedougou, the RT-RPA was operated at an ambient temperature of 38 degrees C with auxiliary electricity tapped from a motor vehicle and yielded a clinical sensitivity and specificity of 98% (n=31) and 100% (n=23), respectively. While in the field trial in Bangkok, the clinical sensitivity and specificity were 72% (n=90) and 100% (n=41), respectively. Conclusions/Significance During the first 5 days of infection, the developed DENV1-4 RT-RPA assays constitute a suitable accurate and rapid assay for DENV diagnosis. Moreover, the use of a portable fluorescence-reading device broadens its application potential to the point-of-care for outbreak investigations."],["dc.identifier.doi","10.1371/journal.pone.0129682"],["dc.identifier.isi","000356329900091"],["dc.identifier.pmid","26075598"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11961"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36842"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]