Tsukanov, Roman

[["dc.bibliographiccitation.firstpage","4541"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Nanoscale Advances"],["dc.bibliographiccitation.lastpage","4553"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Weitzel, Milan"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Oswald, Tabea A."],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Karius, Volker"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2021-08-12T07:45:03Z"],["dc.date.available","2021-08-12T07:45:03Z"],["dc.date.issued","2021"],["dc.description.abstract","The layered silicates Egyptian Blue (CaCuSi 4 O 10 , EB), Han Blue (BaCuSi 4 O 10 , HB) and Han Purple (BaCuSi 2 O 6 , HP) emit as bulk materials bright and stable fluorescence in the near-infrared (NIR), which is of high interest for (bio)photonics due to minimal scattering, absorption and phototoxicity in this spectral range. So far the optical properties of nanosheets (NS) of these silicates are poorly understood. Here, we exfoliate them into monodisperse nanosheets, report their physicochemical properties and use them for (bio)photonics. The approach uses ball milling followed by tip sonication and centrifugation steps to exfoliate the silicates into NS with lateral size and thickness down to ≈ 16–27 nm and 1–4 nm, respectively. They emit at ≈ 927 nm (EB-NS), 953 nm (HB-NS) and 924 nm (HP-NS), and single NS can be imaged in the NIR. The fluorescence lifetimes decrease from ≈ 30–100 μs (bulk) to 17 μs (EB-NS), 8 μs (HB-NS) and 7 μs (HP-NS), thus enabling lifetime-encoded multicolor imaging both on the microscopic and the macroscopic scale. Finally, remote imaging through tissue phantoms reveals the potential for bioimaging. In summary, we report a procedure to gain monodisperse NIR fluorescent silicate nanosheets, determine their size-dependent photophysical properties and showcase the potential for NIR photonics."],["dc.identifier.doi","10.1039/D1NA00238D"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88360"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/324"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2516-0230"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY 3.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/3.0/"],["dc.title","Photophysical properties and fluorescence lifetime imaging of exfoliated near-infrared fluorescent silicate nanosheets"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","48"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Isbaner, Sebastian"],["dc.contributor.author","Eggert Martínez, Mariana"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2020-12-10T18:46:58Z"],["dc.date.available","2020-12-10T18:46:58Z"],["dc.date.issued","2019"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.3390/cells8010048"],["dc.identifier.eissn","2073-4409"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78601"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.publisher","MDPI"],["dc.relation.eissn","2073-4409"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hofemeier, Arne D."],["dc.contributor.author","Limon, Tamara"],["dc.contributor.author","Muenker, Till Moritz"],["dc.contributor.author","Wallmeyer, Bernhard"],["dc.contributor.author","Jurado, Alejandro"],["dc.contributor.author","Afshar, Mohammad Ebrahim"],["dc.contributor.author","Ebrahimi, Majid"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Gilbert, Penney M."],["dc.contributor.author","Betz, Timo"],["dc.date.accessioned","2021-04-14T08:29:33Z"],["dc.date.available","2021-04-14T08:29:33Z"],["dc.date.issued","2021"],["dc.description.abstract","Tension and mechanical properties of muscle tissue are tightly related to proper skeletal muscle function, which makes experimental access to the biomechanics of muscle tissue formation a key requirement to advance our understanding of muscle function and development. Recently developed elastic in vitro culture chambers allow for raising 3D muscle tissue under controlled conditions and to measure global tissue force generation. However, these chambers are inherently incompatible with high-resolution microscopy limiting their usability to global force measurements, and preventing the exploitation of modern fluorescence based investigation methods for live and dynamic measurements. Here, we present a new chamber design pairing global force measurements, quantified from post-deflection, with local tension measurements obtained from elastic hydrogel beads embedded in muscle tissue. High-resolution 3D video microscopy of engineered muscle formation, enabled by the new chamber, shows an early mechanical tissue homeostasis that remains stable in spite of continued myotube maturation."],["dc.identifier.doi","10.7554/eLife.60145"],["dc.identifier.pmid","33459593"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82931"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/118"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2050-084X"],["dc.relation.workinggroup","RG Betz"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY 4.0"],["dc.title","Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3494"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","The Journal of Physical Chemistry. A, Molecules, spectroscopy, kinetics, environment & general theory"],["dc.bibliographiccitation.lastpage","3500"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Weber, André"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Isbaner, Sebastian"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2020-12-10T15:22:42Z"],["dc.date.available","2020-12-10T15:22:42Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1021/acs.jpca.0c01513"],["dc.identifier.pmid","32255633"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73500"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/39"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Wide-Field Fluorescence Lifetime Imaging of Single Molecules"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14190"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","14200"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Helmerich, Dominic A."],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Butkevich, Eugenia"],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2021-03-05T08:58:21Z"],["dc.date.available","2021-03-05T08:58:21Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1021/acsnano.0c07322"],["dc.identifier.pmid","33035050"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80100"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/79"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Helmerich, Dominic"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Butkevich, Eugenia"],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2022-01-12T16:50:38Z"],["dc.date.available","2022-01-12T16:50:38Z"],["dc.date.issued","2020"],["dc.description.abstract","Fluorescence lifetime imaging microscopy (FLIM) is an important technique that adds another dimension to the intensity and colour information of conventional microscopy. In particular, it allows for multiplexing fluorescent labels that have otherwise similar spectral properties. Currently, the only super-resolution technique that is capable of recording super-resolved images with lifetime information is STimulated Emission Depletion (STED) microscopy. In contrast, all Single-Molecule Localisation Microscopy (SMLM) techniques that employ wide-field cameras completely lack the lifetime dimension. Here, we combine Fluorescence-Lifetime Confocal Laser-Scanning Microscopy (FL-CLSM) with SMLM for realising single-molecule localisation-based fluorescence-lifetime super-resolution imaging (FL-SMLM). Besides yielding images with a spatial resolution much beyond the diffraction limit, it determines the fluorescence lifetime of all localised molecules. We validate our technique by applying it to direct STochastic Optical Reconstruction Microscopy (dSTORM) and Points Accumulation for Imaging in Nanoscale Topography (PAINT) imaging of fixed cells, and we demonstrate its multiplexing capability on samples with two different labels that differ only by fluorescence lifetime but not by their spectral properties."],["dc.format.extent","30"],["dc.identifier.doi","10.1101/2020.08.25.266387"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98093"],["dc.language.iso","en"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.title","Confocal Laser-Scanning Fluorescence-Lifetime Single-Molecule Localisation Microscopy"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]