Tsukanov, Roman

[["dc.bibliographiccitation.firstpage","169"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nanophotonics"],["dc.bibliographiccitation.lastpage","202"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Koenderink, A. Femius"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Izeddin, Ignacio"],["dc.contributor.author","Krachmalnicoff, Valentina"],["dc.date.accessioned","2022-02-01T10:31:35Z"],["dc.date.available","2022-02-01T10:31:35Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Probing light–matter interaction at the nanometer scale is one of the most fascinating topics of modern optics. Its importance is underlined by the large span of fields in which such accurate knowledge of light–matter interaction is needed, namely nanophotonics, quantum electrodynamics, atomic physics, biosensing, quantum computing and many more. Increasing innovations in the field of microscopy in the last decade have pushed the ability of observing such phenomena across multiple length scales, from micrometers to nanometers. In bioimaging, the advent of super-resolution single-molecule localization microscopy (SMLM) has opened a completely new perspective for the study and understanding of molecular mechanisms, with unprecedented resolution, which take place inside the cell. Since then, the field of SMLM has been continuously improving, shifting from an initial drive for pushing technological limitations to the acquisition of new knowledge. Interestingly, such developments have become also of great interest for the study of light–matter interaction in nanostructured materials, either dielectric, metallic, or hybrid metallic-dielectric. The purpose of this review is to summarize the recent advances in the field of nanophotonics that have leveraged SMLM, and conversely to show how some concepts commonly used in nanophotonics can benefit the development of new microscopy techniques for biophysics. To this aim, we will first introduce the basic concepts of SMLM and the observables that can be measured. Then, we will link them with their corresponding physical quantities of interest in biophysics and nanophotonics and we will describe state-of-the-art experiments that apply SMLM to nanophotonics. The problem of localization artifacts due to the interaction of the fluorescent emitter with a resonant medium and possible solutions will be also discussed. Then, we will show how the interaction of fluorescent emitters with plasmonic structures can be successfully employed in biology for cell profiling and membrane organization studies. We present an outlook on emerging research directions enabled by the synergy of localization microscopy and nanophotonics."],["dc.identifier.doi","10.1515/nanoph-2021-0551"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98897"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/375"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2192-8614"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY 4.0"],["dc.title","Super-resolution imaging: when biophysics meets nanophotonics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","overview_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4541"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Nanoscale Advances"],["dc.bibliographiccitation.lastpage","4553"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Weitzel, Milan"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Oswald, Tabea A."],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Karius, Volker"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2021-08-12T07:45:03Z"],["dc.date.available","2021-08-12T07:45:03Z"],["dc.date.issued","2021"],["dc.description.abstract","The layered silicates Egyptian Blue (CaCuSi 4 O 10 , EB), Han Blue (BaCuSi 4 O 10 , HB) and Han Purple (BaCuSi 2 O 6 , HP) emit as bulk materials bright and stable fluorescence in the near-infrared (NIR), which is of high interest for (bio)photonics due to minimal scattering, absorption and phototoxicity in this spectral range. So far the optical properties of nanosheets (NS) of these silicates are poorly understood. Here, we exfoliate them into monodisperse nanosheets, report their physicochemical properties and use them for (bio)photonics. The approach uses ball milling followed by tip sonication and centrifugation steps to exfoliate the silicates into NS with lateral size and thickness down to ≈ 16–27 nm and 1–4 nm, respectively. They emit at ≈ 927 nm (EB-NS), 953 nm (HB-NS) and 924 nm (HP-NS), and single NS can be imaged in the NIR. The fluorescence lifetimes decrease from ≈ 30–100 μs (bulk) to 17 μs (EB-NS), 8 μs (HB-NS) and 7 μs (HP-NS), thus enabling lifetime-encoded multicolor imaging both on the microscopic and the macroscopic scale. Finally, remote imaging through tissue phantoms reveals the potential for bioimaging. In summary, we report a procedure to gain monodisperse NIR fluorescent silicate nanosheets, determine their size-dependent photophysical properties and showcase the potential for NIR photonics."],["dc.identifier.doi","10.1039/D1NA00238D"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88360"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/324"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2516-0230"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY 3.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/3.0/"],["dc.title","Photophysical properties and fluorescence lifetime imaging of exfoliated near-infrared fluorescent silicate nanosheets"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","48"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Isbaner, Sebastian"],["dc.contributor.author","Eggert Martínez, Mariana"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2020-12-10T18:46:58Z"],["dc.date.available","2020-12-10T18:46:58Z"],["dc.date.issued","2019"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.3390/cells8010048"],["dc.identifier.eissn","2073-4409"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78601"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.publisher","MDPI"],["dc.relation.eissn","2073-4409"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hofemeier, Arne D."],["dc.contributor.author","Limon, Tamara"],["dc.contributor.author","Muenker, Till Moritz"],["dc.contributor.author","Wallmeyer, Bernhard"],["dc.contributor.author","Jurado, Alejandro"],["dc.contributor.author","Afshar, Mohammad Ebrahim"],["dc.contributor.author","Ebrahimi, Majid"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Gilbert, Penney M."],["dc.contributor.author","Betz, Timo"],["dc.date.accessioned","2021-04-14T08:29:33Z"],["dc.date.available","2021-04-14T08:29:33Z"],["dc.date.issued","2021"],["dc.description.abstract","Tension and mechanical properties of muscle tissue are tightly related to proper skeletal muscle function, which makes experimental access to the biomechanics of muscle tissue formation a key requirement to advance our understanding of muscle function and development. Recently developed elastic in vitro culture chambers allow for raising 3D muscle tissue under controlled conditions and to measure global tissue force generation. However, these chambers are inherently incompatible with high-resolution microscopy limiting their usability to global force measurements, and preventing the exploitation of modern fluorescence based investigation methods for live and dynamic measurements. Here, we present a new chamber design pairing global force measurements, quantified from post-deflection, with local tension measurements obtained from elastic hydrogel beads embedded in muscle tissue. High-resolution 3D video microscopy of engineered muscle formation, enabled by the new chamber, shows an early mechanical tissue homeostasis that remains stable in spite of continued myotube maturation."],["dc.identifier.doi","10.7554/eLife.60145"],["dc.identifier.pmid","33459593"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82931"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/118"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2050-084X"],["dc.relation.workinggroup","RG Betz"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY 4.0"],["dc.title","Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]