Pöggeler, Stefanie

[["dc.bibliographiccitation.firstpage","465"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Eukaryotic Cell"],["dc.bibliographiccitation.lastpage","470"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Hoff, Birgit"],["dc.contributor.author","Poeggeler, Stefanie"],["dc.contributor.author","Kueck, Ulrich"],["dc.date.accessioned","2018-11-07T11:17:28Z"],["dc.date.available","2018-11-07T11:17:28Z"],["dc.date.issued","2008"],["dc.description.abstract","Eighty years ago, Alexander Fleming discovered antibacterial activity in the asexual mold Penicillium, and the strain he studied later was replaced by an overproducing isolate still used for penicillin production today. Using a heterologous PCR approach, we show that these strains are of opposite mating types and that both have retained transcriptionally expressed pheromone and pheromone receptor genes required for sexual reproduction. This discovery extends options for industrial strain improvement programs using conventional genetical approaches."],["dc.identifier.doi","10.1128/EC.00430-07"],["dc.identifier.isi","000258666000005"],["dc.identifier.pmid","18223118"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54814"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","1535-9778"],["dc.title","Eighty years after its discovery, Fleming's Penicillium strain discloses the secret of its sex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6006"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Applied and Environmental Microbiology"],["dc.bibliographiccitation.lastpage","6016"],["dc.bibliographiccitation.volume","74"],["dc.contributor.author","Poeggeler, Stefanie"],["dc.contributor.author","Hoff, Birgit"],["dc.contributor.author","Kueck, Ulrich"],["dc.date.accessioned","2018-11-07T11:10:16Z"],["dc.date.available","2018-11-07T11:10:16Z"],["dc.date.issued","2008"],["dc.description.abstract","Acremonium chrysogenum, the fungal producer of the pharmaceutically relevant beta-lactam antibiotic cephalosporin C, is classified as asexual because no direct observation of mating or meiosis has yet been reported. To assess the potential of A. chrysogenum for sexual reproduction, we screened an expressed sequence tag library from A. chrysogenum for the expression of mating type (MAT) genes, which are the key regulators of sexual reproduction. We identified two putative mating type genes that are homologues of the alpha-box domain gene, MAT1-1-1 and MAT1-1-2, encoding an HPG domain protein defined by the presence of the three invariant amino acids histidine, proline, and glycine. In addition, cDNAs encoding a putative pheromone receptor and pheromone-processing enzymes, as well as components of a pheromone response pathway, were found. Moreover, the entire A. chrysogenum MAT1-1 (AcMAT1-1) gene and regions flanking the MAT region were obtained from a genomic cosmid library, and sequence analysis revealed that in addition to AcMAT1-1-1 and AcMAT1-1-2, the AcMAT1-1 locus comprises a third mating type gene, AcMAT1-1-3, encoding a high-mobility-group domain protein. The alpha-box domain sequence of AcMAT1-1-1 was used to determine the phylogenetic relationships of A. chrysogenum to other ascomycetes. To determine the functionality of the AcMAT1-1 locus, the entire MAT locus was transferred into a MAT deletion strain of the heterothallic ascomycete Podospora anserina (the Pa Delta MAT strain). After fertilization with a P. anserina MAT1-2 (MAT(+)) strain, the corresponding transformants developed fruiting bodies with mature ascospores. Thus, the results of our functional analysis of the AcMAT1-1 locus provide strong evidence to hypothesize a sexual cycle in A. chrysogenum."],["dc.identifier.doi","10.1128/AEM.01188-08"],["dc.identifier.isi","000259528700019"],["dc.identifier.pmid","18689517"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53176"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","0099-2240"],["dc.title","Asexual cephalosporin C producer Acremonium chrysogenum carries a functional mating type locus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","310"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Molecular Microbiology"],["dc.bibliographiccitation.lastpage","323"],["dc.bibliographiccitation.volume","84"],["dc.contributor.author","Bloemendal, Sandra"],["dc.contributor.author","Bernhards, Yasmine"],["dc.contributor.author","Bartho, Kathrin"],["dc.contributor.author","Dettmann, Anne"],["dc.contributor.author","Voigt, Oliver"],["dc.contributor.author","Teichert, Ines"],["dc.contributor.author","Seiler, Stephan"],["dc.contributor.author","Wolters, Dirk A."],["dc.contributor.author","Poeggeler, Stefanie"],["dc.contributor.author","Kueck, Ulrich"],["dc.date.accessioned","2018-11-07T09:11:42Z"],["dc.date.available","2018-11-07T09:11:42Z"],["dc.date.issued","2012"],["dc.description.abstract","Sexual development in fungi is a complex process involving the generation of new cell types and tissues an essential step for all eukaryotic life. The characterization of sterile mutants in the ascomycete Sordaria macrospora has led to a number of proteins involved in sexual development, but a link between these proteins is still missing. Using a combined tandem-affinity purification/mass spectrometry approach, we showed in vivo association of developmental protein PRO22 with PRO11, homologue of mammalian striatin, and SmPP2AA, scaffolding subunit of protein phosphatase 2A. Further experiments extended the protein network to the putative kinase activator SmMOB3, known to be involved in sexual development. Extensive yeast two-hybrid studies allowed us to pinpoint functional domains involved in proteinprotein interaction. We show for the first time that a number of already known factors together with new components associate in vivo to form a highly conserved multi-subunit complex. Strikingly, a similar complex has been described in humans, but the function of this so-called striatin interacting phosphatase and kinase (STRIPAK) complex is largely unknown. In S. macrospora, truncation of PRO11 and PRO22 leads to distinct defects in sexual development and cell fusion, indicating a role for the fungal STRIPAK complex in both processes."],["dc.identifier.doi","10.1111/j.1365-2958.2012.08024.x"],["dc.identifier.isi","000302539400008"],["dc.identifier.pmid","22375702"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26780"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1365-2958"],["dc.relation.issn","0950-382X"],["dc.title","A homologue of the human STRIPAK complex controls sexual development in fungi"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1476"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","1481"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Boehm, Julia"],["dc.contributor.author","Hoff, Birgit"],["dc.contributor.author","O'Gorman, Celine M."],["dc.contributor.author","Wolfers, Simon"],["dc.contributor.author","Klix, Volker"],["dc.contributor.author","Binger, Danielle"],["dc.contributor.author","Zadra, Ivo"],["dc.contributor.author","Kuernsteiner, Hubert"],["dc.contributor.author","Poeggeler, Stefanie"],["dc.contributor.author","Dyer, Paul S."],["dc.contributor.author","Kueck, Ulrich"],["dc.date.accessioned","2018-11-07T09:29:04Z"],["dc.date.available","2018-11-07T09:29:04Z"],["dc.date.issued","2013"],["dc.description.abstract","Penicillium chrysogenum is a filamentous fungus of major medical and historical importance, being the original and present-day industrial source of the antibiotic penicillin. The species has been considered asexual for more than 100 y, and despite concerted efforts, it has not been possible to induce sexual reproduction, which has prevented sexual crosses being used for strain improvement. However, using knowledge of mating-type (MAT) gene organization, we now describe conditions under which a sexual cycle can be induced leading to production of meiotic ascospores. Evidence of recombination was obtained using both molecular and phenotypic markers. The identified heterothallic sexual cycle was used for strain development purposes, generating offspring with novel combinations of traits relevant to penicillin production. Furthermore, the MAT1-1-1 mating-type gene, known primarily for a role in governing sexual identity, was also found to control transcription of a wide range of genes with biotechnological relevance including those regulating penicillin production, hyphal morphology, and conidial formation. These discoveries of a sexual cycle and MAT gene function are likely to be of broad relevance for manipulation of other asexual fungi of economic importance."],["dc.description.sponsorship","Sandoz GmbH; Christian Doppler Society; Wellcome Trust"],["dc.identifier.doi","10.1073/pnas.1217943110"],["dc.identifier.isi","000314453900068"],["dc.identifier.pmid","23307807"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30930"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.title","Sexual reproduction and mating-type-mediated strain development in the penicillin-producing fungus Penicillium chrysogenum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","199"],["dc.bibliographiccitation.journal","Advances in Genetics"],["dc.bibliographiccitation.lastpage","244"],["dc.bibliographiccitation.volume","87"],["dc.contributor.author","Teichert, Ines"],["dc.contributor.author","Nowrousian, Minou"],["dc.contributor.author","Poeggeler, Stefanie"],["dc.contributor.author","Kueck, Ulrich"],["dc.date.accessioned","2018-11-07T09:45:21Z"],["dc.date.available","2018-11-07T09:45:21Z"],["dc.date.issued","2014"],["dc.description.abstract","Filamentous fungi are excellent experimental systems due to their short life cycles as well as easy and safe manipulation in the laboratory. They form three-dimensional structures with numerous different cell types and have a long tradition as genetic model organisms used to unravel basic mechanisms underlying eukaryotic cell differentiation. The filamentous ascomycete Sordaria macrospora is a model system for sexual fruiting body (perithecia) formation. S. macrospora is homothallic, i.e., self-fertile, easily genetically tractable, and well suited for large-scale genomics, transcriptomics, and proteomics studies. Specific features of its life cycle and the availability of a developmental mutant library make it an excellent system for studying cellular differentiation at the molecular level. In this review, we focus on recent developments in identifying gene and protein regulatory networks governing perithecia formation. A number of tools have been developed to genetically analyze developmental mutants and dissect transcriptional profiles at different developmental stages. Protein interaction studies allowed us to identify a highly conserved eukaryotic multisubunit protein complex, the striatin-interacting phosphatase and kinase complex and its role in sexual development. We have further identified a number of proteins involved in chromatin remodeling and transcriptional regulation of fruiting body development. Furthermore, we review the involvement of metabolic processes from both primary and secondary metabolism, and the role of nutrient recycling by autophagy in perithecia formation. Our research has uncovered numerous players regulating multicellular development in S. macrospora. Future research will focus on mechanistically understanding how these players are orchestrated in this fungal model system."],["dc.identifier.doi","10.1016/B978-0-12-800149-3.00004-4"],["dc.identifier.isi","000349890200004"],["dc.identifier.pmid","25311923"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34596"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.isbn","978-0-12-800149-3"],["dc.relation.issn","0065-2660"],["dc.title","The Filamentous Fungus Sordaria macrospora as a Genetic Model to Study Fruiting Body Development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","615"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Fungal Biology"],["dc.bibliographiccitation.lastpage","624"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Poeggeler, Stefanie"],["dc.contributor.author","O'Gorman, Celine M."],["dc.contributor.author","Hoff, Birgit"],["dc.contributor.author","Kueck, Ulrich"],["dc.date.accessioned","2018-11-07T08:54:32Z"],["dc.date.available","2018-11-07T08:54:32Z"],["dc.date.issued","2011"],["dc.description.abstract","Eupenicillium species are the teleomorphic (sexual) forms of anamorphic (asexual) members of the genus Penicillium, which contains many species of industrial importance. Here we describe the first molecular analysis of the mating-type (MAT) locus from a homothallic (self-fertile) Eupenicillium species, E. crustaceum. This ascomycete is a sexual relative of the penicillin producer Penicillium chrysogenum, which while long considered asexual, was recently shown to possess the required genetic machinery for heterothallic breeding. The E. crustaceum genome contains two MAT loci, MAT1-1 and MAT1-2, in an arrangement characteristic of other known homothallic euascomycetes, such as Neosartorya fischeri. MAT1-1 is flanked by conserved APN2 (DNA lyase) and SLA2 (cytoskeleton assembly control) genes and encodes a homologue of the alpha-box domain protein MAT1-1-1. Conversely, MAT1-2 carries a HMG-domain gene MAT1-2-1, and is flanked by a degenerate SLA2 gene and an intact homologue of the P. chrysogenum ORF Pc20g08960. Here we demonstrate the transcriptional expression of both mating-type genes during vegetative development. Furthermore, the MAT1-1-1 and MAT1-2-1 sequences were used to resolve the phylogenetic relationship of E. crustaceum with other ascomycetes. Phylogenetic trees confirmed a very close relationship between the homothallic E. crustaceum and the supposedly heterothallic P. chrysogenum. This close taxonomic association makes E. crustaceum an ideal candidate for future expression and evolutionary studies of sexual reproduction, with the ultimate aim of inducing sex in P. chrysogenum. (C) 2011 The British Mycological Society. Published by Elsevier Ltd. All rights reserved."],["dc.identifier.doi","10.1016/j.funbio.2011.03.003"],["dc.identifier.isi","000293421800004"],["dc.identifier.pmid","21724167"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22693"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Sci Ltd"],["dc.relation.issn","1878-6146"],["dc.title","Molecular organization of the mating-type loci in the homothallic Ascomycete Eupenicillium crustaceum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1000891"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PLoS Genetics"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Nowrousian, Minou"],["dc.contributor.author","Stajich, Jason E."],["dc.contributor.author","Chu, Meiling"],["dc.contributor.author","Engh, Ines"],["dc.contributor.author","Espagne, Eric"],["dc.contributor.author","Halliday, Karen"],["dc.contributor.author","Kamerewerd, Jens"],["dc.contributor.author","Kempken, Frank"],["dc.contributor.author","Knab, Birgit"],["dc.contributor.author","Kuo, Hsiao-Che"],["dc.contributor.author","Osiewacz, Heinz D."],["dc.contributor.author","Poeggeler, Stefanie"],["dc.contributor.author","Read, Nick D."],["dc.contributor.author","Seiler, Stephan"],["dc.contributor.author","Smith, Kristina M."],["dc.contributor.author","Zickler, Denise"],["dc.contributor.author","Kueck, Ulrich"],["dc.contributor.author","Freitag, Michael"],["dc.date.accessioned","2018-11-07T08:44:49Z"],["dc.date.available","2018-11-07T08:44:49Z"],["dc.date.issued","2010"],["dc.description.abstract","Filamentous fungi are of great importance in ecology, agriculture, medicine, and biotechnology. Thus, it is not surprising that genomes for more than 100 filamentous fungi have been sequenced, most of them by Sanger sequencing. While next-generation sequencing techniques have revolutionized genome resequencing, e. g. for strain comparisons, genetic mapping, or transcriptome and ChIP analyses, de novo assembly of eukaryotic genomes still presents significant hurdles, because of their large size and stretches of repetitive sequences. Filamentous fungi contain few repetitive regions in their 30-90 Mb genomes and thus are suitable candidates to test de novo genome assembly from short sequence reads. Here, we present a high-quality draft sequence of the Sordaria macrospora genome that was obtained by a combination of Illumina/Solexa and Roche/454 sequencing. Paired-end Solexa sequencing of genomic DNA to 85-fold coverage and an additional 10-fold coverage by single-end 454 sequencing resulted in similar to 4 Gb of DNA sequence. Reads were assembled to a 40 Mb draft version (N50 of 117 kb) with the Velvet assembler. Comparative analysis with Neurospora genomes increased the N50 to 498 kb. The S. macrospora genome contains even fewer repeat regions than its closest sequenced relative, Neurospora crassa. Comparison with genomes of other fungi showed that S. macrospora, a model organism for morphogenesis and meiosis, harbors duplications of several genes involved in self/nonself-recognition. Furthermore, S. macrospora contains more polyketide biosynthesis genes than N. crassa. Phylogenetic analyses suggest that some of these genes may have been acquired by horizontal gene transfer from a distantly related ascomycete group. Our study shows that, for typical filamentous fungi, de novo assembly of genomes from short sequence reads alone is feasible, that a mixture of Solexa and 454 sequencing substantially improves the assembly, and that the resulting data can be used for comparative studies to address basic questions of fungal biology."],["dc.identifier.doi","10.1371/journal.pgen.1000891"],["dc.identifier.isi","000277354200008"],["dc.identifier.pmid","20386741"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7264"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20286"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1553-7390"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","De novo Assembly of a 40 Mb Eukaryotic Genome from Short Sequence Reads: Sordaria macrospora, a Model Organism for Fungal Morphogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","191"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Genetics"],["dc.bibliographiccitation.lastpage","206"],["dc.bibliographiccitation.volume","180"],["dc.contributor.author","Kamerewerd, Jens"],["dc.contributor.author","Jansson, Malin"],["dc.contributor.author","Nowrousian, Minou"],["dc.contributor.author","Poeggeler, Stefanie"],["dc.contributor.author","Kueck, Ulrich"],["dc.date.accessioned","2018-11-07T11:11:29Z"],["dc.date.available","2018-11-07T11:11:29Z"],["dc.date.issued","2008"],["dc.description.abstract","Sordaria macrospora, a self-fertile filamentous ascomycete, carries genes encoding three different alpha-subunits of heterotrimeric G proteins (gsa, G protein Sordaria alpha Subunit). We generated knockout strains for all three gsagenes (Delta gsa1, Delta gsa2, and Delta gsa3) as well as all combinations of double mutants. Phenotypic analysis of single and double mutants showed that the genes for G alpha-subunits have distinct roles in the sexual life cycle. While single Mutants show some reduction of fertility, double mutants Delta gsa1 Delta gsa2 and Delta gsa1 Delta gsa3 are completely sterile. To test whether the pheromone receptors PRE1 and PRE2 mediate signaling via distinct G alpha-subunits, two recently generated Delta pre strains were crossed with all Delta gsa strains. Analyses of the corresponding double mutants revealed that compared to GSA2, GSA1 is a more predominant regulator of a signal transduction cascade downstream of the pheromone receptors and that GSA3 is involved in another signaling pathway that also contributes to fruiting body development and fertility. We further isolated the gene encoding adenylyl cyclase (AC) (sac]) for construction of a knockout strain. Analyses of the three Delta gsa Delta sac1 double mutants and one Delta gsa2 Delta gsa3 Delta sac1 triple mutant indicate that SAC1 acts downstream of GSA3, parallel to a GSA1-GSA2-mediated signaling pathway. In addition, the function of STE12 and PRO41, two presumptive signaling components, was investigated in diverse double mutants lacking those developmental genes in combination with the gsa genes. This analysis was further completed by expression Studies of the ste12 and pro41 transcripts in wild-type and mutant strains. From the SLIM of all our data, we propose a model for how different G alpha-subunits interact with pheromone receptors, adenylyl cyclase, and STE12 and thus cooperatively regulate sexual development in S. macrospora."],["dc.identifier.doi","10.1534/genetics.108.091603"],["dc.identifier.isi","000259758500017"],["dc.identifier.pmid","18723884"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53445"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Genetics"],["dc.relation.issn","0016-6731"],["dc.title","Three alpha-subunits of heterotrimeric G proteins and an adenylyl cyclase have distinct roles in fruiting body development in the homothallic fungus Sordaria macrospora"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]