Pöggeler, Stefanie

[["dc.bibliographiccitation.artnumber","e1003820"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PLoS Genetics"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Traeger, Stefanie"],["dc.contributor.author","Altegoer, Florian"],["dc.contributor.author","Freitag, Michael"],["dc.contributor.author","Gabaldon, Toni"],["dc.contributor.author","Kempken, Frank"],["dc.contributor.author","Kumar, Abhishek"],["dc.contributor.author","Marcet-Houben, Marina"],["dc.contributor.author","Poeggeler, Stefanie"],["dc.contributor.author","Stajich, Jason E."],["dc.contributor.author","Nowrousian, Minou"],["dc.date.accessioned","2018-11-07T09:20:30Z"],["dc.date.available","2018-11-07T09:20:30Z"],["dc.date.issued","2013"],["dc.description.abstract","Fungi are a large group of eukaryotes found in nearly all ecosystems. More than 250 fungal genomes have already been sequenced, greatly improving our understanding of fungal evolution, physiology, and development. However, for the Pezizomycetes, an early-diverging lineage of filamentous ascomycetes, there is so far only one genome available, namely that of the black truffle, Tuber melanosporum, a mycorrhizal species with unusual subterranean fruiting bodies. To help close the sequence gap among basal filamentous ascomycetes, and to allow conclusions about the evolution of fungal development, we sequenced the genome and assayed transcriptomes during development of Pyronema confluens, a saprobic Pezizomycete with a typical apothecium as fruiting body. With a size of 50 Mb and similar to 13,400 protein-coding genes, the genome is more characteristic of higher filamentous ascomycetes than the large, repeat-rich truffle genome; however, some typical features are different in the P. confluens lineage, e.g. the genomic environment of the mating type genes that is conserved in higher filamentous ascomycetes, but only partly conserved in P. confluens. On the other hand, P. confluens has a full complement of fungal photoreceptors, and expression studies indicate that light perception might be similar to distantly related ascomycetes and, thus, represent a basic feature of filamentous ascomycetes. Analysis of spliced RNA-seq sequence reads allowed the detection of natural antisense transcripts for 281 genes. The P. confluens genome contains an unusually high number of predicted orphan genes, many of which are upregulated during sexual development, consistent with the idea of rapid evolution of sex-associated genes. Comparative transcriptomics identified the transcription factor gene pro44 that is upregulated during development in P. confluens and the Sordariomycete Sordaria macrospora. The P. confluens pro44 gene (PCON_06721) was used to complement the S. macrospora pro44 deletion mutant, showing functional conservation of this developmental regulator."],["dc.identifier.doi","10.1371/journal.pgen.1003820"],["dc.identifier.isi","000325076600074"],["dc.identifier.pmid","24068976"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9290"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28893"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1553-7404"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","The Genome and Development-Dependent Transcriptomes of Pyronema confluens: A Window into Fungal Evolution"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0157960"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Werner, Antonia"],["dc.contributor.author","Herzog, Britta"],["dc.contributor.author","Frey, Stefan"],["dc.contributor.author","Poeggeler, Stefanie"],["dc.date.accessioned","2018-11-07T10:12:44Z"],["dc.date.available","2018-11-07T10:12:44Z"],["dc.date.issued","2016"],["dc.description.abstract","In filamentous fungi, autophagy functions as a catabolic mechanism to overcome starvation and to control diverse developmental processes under normal nutritional conditions. Autophagy involves the formation of double-membrane vesicles, termed autophagosomes that engulf cellular components and bring about their degradation via fusion with vacuoles. Two ubiquitin-like (UBL) conjugation systems are essential for the expansion of the autophagosomal membrane: the UBL protein ATG8 is conjugated to the lipid phosphatidylethanolamine and the UBL protein ATG12 is coupled to ATG5. We recently showed that in the homothallic ascomycete Sordaria macrospora autophagy-related genes encoding components of the conjugation systems are required for fruiting-body development and/or are essential for viability. In the present work, we cloned and characterized the S. macrospora (Sm)atg12 gene. Two-hybrid analysis revealed that SmATG12 can interact with SmATG7 and SmATG3. To examine its role in S. macrospora, we replaced the open reading frame of Smatg12 with a hygromycin resistance cassette and generated a homokaryotic Delta Smatg12 knockout strain, which displayed slower vegetative growth under nutrient starvation conditions and was unable to form fruiting bodies. In the hyphae of S. macrospora EGFP-labeled SmATG12 was detected in the cytoplasm and as punctate structures presumed to be phagophores or phagophore assembly sites. Delivery of EGFP-labelled SmATG8 to the vacuole was entirely dependent on SmATG12."],["dc.description.sponsorship","Open-Access Publikationsfonds 2016"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.1371/journal.pone.0157960"],["dc.identifier.isi","000378029800144"],["dc.identifier.pmid","27309377"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13387"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40297"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Autophagy-Associated Protein SmATG12 Is Required for Fruiting-Body Formation in the Filamentous Ascomycete Sordaria macrospora"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","93"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Metabolites"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Vullo, Daniela"],["dc.contributor.author","Lehneck, Ronny"],["dc.contributor.author","Donald, William A."],["dc.contributor.author","Pöggeler, Stefanie"],["dc.contributor.author","Supuran, Claudiu T."],["dc.date.accessioned","2020-12-10T18:47:15Z"],["dc.date.available","2020-12-10T18:47:15Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/metabo10030093"],["dc.identifier.eissn","2218-1989"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78697"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.publisher","MDPI"],["dc.relation.eissn","2218-1989"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Anion Inhibition Studies of the β-Class Carbonic Anhydrase CAS3 from the Filamentous Ascomycete Sordaria macrospora"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","580"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Fungi"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Groth, Anika"],["dc.contributor.author","Schunke, Carolin"],["dc.contributor.author","Reschka, Eva"],["dc.contributor.author","Pöggeler, Stefanie"],["dc.contributor.author","Nordzieke, Daniela"],["dc.date.accessioned","2021-08-12T07:46:01Z"],["dc.date.available","2021-08-12T07:46:01Z"],["dc.date.issued","2021"],["dc.description.abstract","Polar growth is a key characteristic of all filamentous fungi. It allows these eukaryotes to not only effectively explore organic matter but also interact within its own colony, mating partners, and hosts. Therefore, a detailed understanding of the dynamics in polar growth establishment and maintenance is crucial for several fields of fungal research. We developed a new marker protein, the actin-related protein 1 (Arp1) fused to red and green fluorescent proteins, which allows for the tracking of polar axis establishment and active hyphal growth in microscopy approaches. To exclude a probable redundancy with known polarity markers, we compared the localizations of the Spitzenkörper (SPK) and Arp1 using an FM4-64 staining approach. As we show in applications with the coprophilous fungus Sordaria macrospora and the hemibiotrophic plant pathogen Colletotrichum graminicola, the monitoring of Arp1 can be used for detailed studies of hyphal growth dynamics and ascospore germination, the interpretation of chemotropic growth processes, and the tracking of elongating penetration pegs into plant material. Since the Arp1 marker showed the same dynamics in both fungi tested, we believe this marker can be broadly applied in fungal research to study the manifold polar growth processes determining fungal life."],["dc.description.abstract","Polar growth is a key characteristic of all filamentous fungi. It allows these eukaryotes to not only effectively explore organic matter but also interact within its own colony, mating partners, and hosts. Therefore, a detailed understanding of the dynamics in polar growth establishment and maintenance is crucial for several fields of fungal research. We developed a new marker protein, the actin-related protein 1 (Arp1) fused to red and green fluorescent proteins, which allows for the tracking of polar axis establishment and active hyphal growth in microscopy approaches. To exclude a probable redundancy with known polarity markers, we compared the localizations of the Spitzenkörper (SPK) and Arp1 using an FM4-64 staining approach. As we show in applications with the coprophilous fungus Sordaria macrospora and the hemibiotrophic plant pathogen Colletotrichum graminicola, the monitoring of Arp1 can be used for detailed studies of hyphal growth dynamics and ascospore germination, the interpretation of chemotropic growth processes, and the tracking of elongating penetration pegs into plant material. Since the Arp1 marker showed the same dynamics in both fungi tested, we believe this marker can be broadly applied in fungal research to study the manifold polar growth processes determining fungal life."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG)"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/jof7070580"],["dc.identifier.pii","jof7070580"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88597"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation.eissn","2309-608X"],["dc.relation.orgunit","Abteilung Genetik eukaryotischer Mikroorganismen"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","682"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Journal of Fungi"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Groth, Anika"],["dc.contributor.author","Schmitt, Kerstin"],["dc.contributor.author","Valerius, Oliver"],["dc.contributor.author","Herzog, Britta"],["dc.contributor.author","Pöggeler, Stefanie"],["dc.date.accessioned","2021-12-01T09:24:06Z"],["dc.date.available","2021-12-01T09:24:06Z"],["dc.date.issued","2021"],["dc.description.abstract","In the filamentous fungus Sordaria macrospora (Sm), the STRIPAK complex is required for vegetative growth, fruiting-body development and hyphal fusion. The SmSTRIPAK core consists of the striatin homolog PRO11, the scaffolding subunit of phosphatase PP2A, SmPP2AA, and its catalytic subunit SmPP2Ac1. Among other STRIPAK proteins, the recently identified coiled-coil protein SCI1 was demonstrated to co-localize around the nucleus. Pulldown experiments with SCI identified the transmembrane nucleoporin (TM Nup) SmPOM33 as a potential nuclear-anchor of SmSTRIPAK. Localization studies revealed that SmPOM33 partially localizes to the nuclear envelope (NE), but mainly to the endoplasmic reticulum (ER). We succeeded to generate a Δpom33 deletion mutant by homologous recombination in a new S. macrospora Δku80 recipient strain, which is defective in non-homologous end joining. Deletion of Smpom33 did neither impair vegetative growth nor sexual development. In pulldown experiments of SmPOM33 followed by LC/MS analysis, ER-membrane proteins involved in ER morphology, protein translocation, glycosylation, sterol biosynthesis and Ca2+-transport were significantly enriched. Data are available via ProteomeXchange with identifier PXD026253. Although no SmSTRIPAK components were identified as putative interaction partners, it cannot be excluded that SmPOM33 is involved in temporarily anchoring the SmSTRIPAK to the NE or other sites in the cell."],["dc.description.abstract","In the filamentous fungus Sordaria macrospora (Sm), the STRIPAK complex is required for vegetative growth, fruiting-body development and hyphal fusion. The SmSTRIPAK core consists of the striatin homolog PRO11, the scaffolding subunit of phosphatase PP2A, SmPP2AA, and its catalytic subunit SmPP2Ac1. Among other STRIPAK proteins, the recently identified coiled-coil protein SCI1 was demonstrated to co-localize around the nucleus. Pulldown experiments with SCI identified the transmembrane nucleoporin (TM Nup) SmPOM33 as a potential nuclear-anchor of SmSTRIPAK. Localization studies revealed that SmPOM33 partially localizes to the nuclear envelope (NE), but mainly to the endoplasmic reticulum (ER). We succeeded to generate a Δpom33 deletion mutant by homologous recombination in a new S. macrospora Δku80 recipient strain, which is defective in non-homologous end joining. Deletion of Smpom33 did neither impair vegetative growth nor sexual development. In pulldown experiments of SmPOM33 followed by LC/MS analysis, ER-membrane proteins involved in ER morphology, protein translocation, glycosylation, sterol biosynthesis and Ca2+-transport were significantly enriched. Data are available via ProteomeXchange with identifier PXD026253. Although no SmSTRIPAK components were identified as putative interaction partners, it cannot be excluded that SmPOM33 is involved in temporarily anchoring the SmSTRIPAK to the NE or other sites in the cell."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/jof7090682"],["dc.identifier.pii","jof7090682"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94847"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","2309-608X"],["dc.relation.orgunit","Institut für Mikrobiologie und Genetik"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Analysis of the Putative Nucleoporin POM33 in the Filamentous Fungus Sordaria macrospora"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e5177"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Elleuche, Skander"],["dc.contributor.author","Poeggeler, Stefanie"],["dc.date.accessioned","2018-11-07T08:30:47Z"],["dc.date.available","2018-11-07T08:30:47Z"],["dc.date.issued","2009"],["dc.description.abstract","Carbon dioxide (CO(2)) is among the most important gases for all organisms. Its reversible interconversion to bicarbonate (HCO(3)(-)) reaches equilibrium spontaneously, but slowly, and can be accelerated by a ubiquitous group of enzymes called carbonic anhydrases (CAs). These enzymes are grouped by their distinct structural features into alpha-, beta-, gamma-, delta-and zeta-classes. While physiological functions of mammalian, prokaryotic, plant and algal CAs have been extensively studied over the past years, the role of beta-CAs in yeasts and the human pathogen Cryptococcus neoformans has been elucidated only recently, and the function of CAs in multicellular filamentous ascomycetes is mostly unknown. To assess the role of CAs in the development of filamentous ascomycetes, the function of three genes, cas1, cas2 and cas3 (carbonic anhydrase of Sordaria) encoding beta-class carbonic anhydrases was characterized in the filamentous ascomycetous fungus Sordaria macrospora. Fluorescence microscopy was used to determine the localization of GFP- and DsRED-tagged CAs. While CAS1 and CAS3 are cytoplasmic enzymes, CAS2 is localized to the mitochondria. To assess the function of the three isoenzymes, we generated knock-out strains for all three cas genes (Delta cas1, Delta cas2, and Delta cas3) as well as all combinations of double mutants. No effect on vegetative growth, fruiting-body and ascospore development was seen in the single mutant strains lacking cas1 or cas3, while single mutant Delta cas2 was affected in vegetative growth, fruiting-body development and ascospore germination, and the double mutant strain Delta cas1/2 was completely sterile. Defects caused by the lack of cas2 could be partially complemented by elevated CO(2) levels or overexpression of cas1, cas3, or a non-mitochondrial cas2 variant. The results suggest that CAs are required for sexual reproduction in filamentous ascomycetes and that the multiplicity of isoforms results in redundancy of specific and non-specific functions."],["dc.identifier.doi","10.1371/journal.pone.0005177"],["dc.identifier.isi","000265509900007"],["dc.identifier.pmid","19365544"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8275"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16974"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","beta-Carbonic Anhydrases Play a Role in Fruiting Body Development and Ascospore Germination in the Filamentous Fungus Sordaria macrospora"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Molecular Plant Pathology"],["dc.contributor.author","Harting, Rebekka"],["dc.contributor.author","Starke, Jessica"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Pöggeler, Stefanie"],["dc.contributor.author","Maurus, Isabel"],["dc.contributor.author","Schlüter, Rabea"],["dc.contributor.author","Landesfeind, Manuel"],["dc.contributor.author","Bulla, Ingo"],["dc.contributor.author","Nowrousian, Minou"],["dc.contributor.author","Braus, Gerhard H."],["dc.contributor.author","de Jonge, Ronnie"],["dc.contributor.author","Stahlhut, Gertrud"],["dc.contributor.author","Hoff, Katharina J."],["dc.contributor.author","Aßhauer, Kathrin P."],["dc.contributor.author","Thürmer, Andrea"],["dc.contributor.author","Stanke, Mario"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Morgenstern, Burkhard"],["dc.contributor.author","Thomma, Bart P. H. J."],["dc.contributor.author","Kronstad, James W."],["dc.contributor.author","Braus‐Stromeyer, Susanna A."],["dc.date.accessioned","2021-06-01T09:42:04Z"],["dc.date.available","2021-06-01T09:42:04Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Amphidiploid fungal Verticillium longisporum strains Vl43 and Vl32 colonize the plant host Brassica napus but differ in their ability to cause disease symptoms. These strains represent two V. longisporum lineages derived from different hybridization events of haploid parental Verticillium strains. Vl32 and Vl43 carry same‐sex mating‐type genes derived from both parental lineages. Vl32 and Vl43 similarly colonize and penetrate plant roots, but asymptomatic Vl32 proliferation in planta is lower than virulent Vl43. The highly conserved Vl43 and Vl32 genomes include less than 1% unique genes, and the karyotypes of 15 or 16 chromosomes display changed genetic synteny due to substantial genomic reshuffling. A 20 kb Vl43 lineage‐specific (LS) region apparently originating from the Verticillium dahliae‐related ancestor is specific for symptomatic Vl43 and encodes seven genes, including two putative transcription factors. Either partial or complete deletion of this LS region in Vl43 did not reduce virulence but led to induction of even more severe disease symptoms in rapeseed. This suggests that the LS insertion in the genome of symptomatic V. longisporum Vl43 mediates virulence‐reducing functions, limits damage on the host plant, and therefore tames Vl43 from being even more virulent."],["dc.description.abstract","A lineage‐specific region in the Verticillium longisporum Vl43 genome reduces fungal virulence of infected rapeseed host plants. image"],["dc.description.sponsorship","Bundesministerium für Bildung und Forschung: BioFung http://dx.doi.org/10.13039/501100002347"],["dc.description.sponsorship","Natural Sciences and Engineering Research Council of Canada: CREATE http://dx.doi.org/10.13039/501100000038"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1111/mpp.13071"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85132"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1364-3703"],["dc.relation.issn","1464-6722"],["dc.relation.orgunit","Abteilung Molekulare Mikrobiologie & Genetik"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","82"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Fungi"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Werner, Antonia"],["dc.contributor.author","Otte, Kolja"],["dc.contributor.author","Stahlhut, Gertrud"],["dc.contributor.author","Hanke, Leon M."],["dc.contributor.author","Pöggeler, Stefanie"],["dc.date.accessioned","2021-04-14T08:27:53Z"],["dc.date.available","2021-04-14T08:27:53Z"],["dc.date.issued","2021"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.3390/jof7020082"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82441"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","MDPI"],["dc.relation.eissn","2309-608X"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","The Glyoxysomal Protease LON2 Is Involved in Fruiting-Body Development, Ascosporogenesis and Stress Resistance in Sordaria macrospora"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Schunke, Carolin"],["dc.contributor.author","Pöggeler, Stefanie"],["dc.contributor.author","Nordzieke, Daniela Elisabeth"],["dc.date.accessioned","2021-04-14T08:31:11Z"],["dc.date.available","2021-04-14T08:31:11Z"],["dc.date.issued","2020"],["dc.description.abstract","Chemical gradients are surrounding living organisms in all habitats of life. Microorganisms, plants and animals have developed specific mechanisms to sense such gradients. Upon perception, chemical gradients can be categorized either as favorable, like nutrients or hormones, or as disadvantageous, resulting in a clear orientation toward the gradient and avoiding strategies, respectively. Being sessile organisms, fungi use chemical gradients for their orientation in the environment. Integration of this data enables them to successfully explore nutrient sources, identify probable plant or animal hosts, and to communicate during sexual reproduction or early colony development. We have developed a 3D printed device allowing a highly standardized, rapid and low-cost investigation of chemotropic growth processes in fungi. Since the 3D printed device is placed on a microscope slide, detailed microscopic investigations and documentation of the chemotropic process is possible. Using this device, we provide evidence that germlings derived from oval conidia of the hemibiotrophic plant pathogen Colletotrichum graminicola can sense gradients of glucose and reorient their growth toward the nutrient source. We describe in detail the method establishment, probable pitfalls, and provide the original program files for 3D printing to enable broad application of the 3D device in basic, agricultural, medical, and applied fungal science."],["dc.identifier.doi","10.3389/fmicb.2020.584525"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17646"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83511"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-302X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","A 3D Printed Device for Easy and Reliable Quantification of Fungal Chemotropic Growth"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1036"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Molecules"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Vullo, Daniela"],["dc.contributor.author","Lehneck, Ronny"],["dc.contributor.author","Donald, William A."],["dc.contributor.author","Pöggeler, Stefanie"],["dc.contributor.author","Supuran, Claudiu T."],["dc.date.accessioned","2020-12-10T18:47:17Z"],["dc.date.available","2020-12-10T18:47:17Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/molecules25051036"],["dc.identifier.eissn","1420-3049"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78706"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.publisher","MDPI"],["dc.relation.eissn","1420-3049"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Sulfonamide Inhibition Studies of the β-Class Carbonic Anhydrase CAS3 from the Filamentous Ascomycete Sordaria macrospora"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]