Now showing 1 - 2 of 2
  • 2013Journal Article
    [["dc.bibliographiccitation.firstpage","866"],["dc.bibliographiccitation.issue","09"],["dc.bibliographiccitation.journal","Advances in Bioscience and Biotechnology"],["dc.bibliographiccitation.lastpage","871"],["dc.bibliographiccitation.volume","04"],["dc.contributor.author","Mushtaq, Asim"],["dc.contributor.author","Tariq, Mujahid Azeem"],["dc.contributor.author","Rasheed, Umer"],["dc.contributor.author","Afroz, Amber"],["dc.contributor.author","Zeeshan, Nadia"],["dc.contributor.author","Asif, Abdul R."],["dc.contributor.author","Zahur, Muzna"],["dc.date.accessioned","2019-07-09T11:54:34Z"],["dc.date.available","2019-07-09T11:54:34Z"],["dc.date.issued","2013"],["dc.description.abstract","Hepatitis C has a 3% of the global disease burden that remains endemic in many regions of the world. According to a general statistical survey it has approximately 5.3% seroprevalence in Pakistan. HCV is a persistent and silent disease thus making the primary diagnosis complicated. Occasionally HCV positive population could not be diagnosed by routine HCV antibody testing therefore requires molecular diagnosis. This study is aimed to determine the prevalence of HCV and to estimate the HCV viral load by quantitative analysis among different patient groups of District Gujrat, Pakistan. A total of 597 samples were collected from clinically diagnosed liver patients that were categorized into three age groups: 1\\) up to 25 years; 2\\) 26-50 years 3\\) above 50 years. All samples were subjected to real time PCR for determination and quantification of HCV RNA. Activity of liver aminotransferases was measured. The overall prevalence of HCV-RNA was 73.87%. Females had slightly higher HCV prevalence which is 74.06% while 73.45% in males. Highest prevalence of active HCV infection was found in age group 26-50. In addition, liver function tests showed that 28.12% HCV-positive patients do not have elevated ALT level whereas 32.65% did not show elevated AST levels. It may be assumed that there is not a significant relationship between increased viral load and liver amino transferases. The study concluded a significantly higher rate of HCV infection in young population. Moreover screening with antibody and liver function tests alone does not exclude the possibility of HCV infection."],["dc.format.extent","6"],["dc.identifier.doi","10.4236/abb.2013.49115"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9300"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60680"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2156-8502"],["dc.rights","CC BY-NC 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/3.0"],["dc.title","Estimation of HCV viral load and liver enzymes among different patients groups of District Gujrat, Pakistan"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
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  • 2018Journal Article
    [["dc.bibliographiccitation.firstpage","29"],["dc.bibliographiccitation.journal","Electronic Journal of Biotechnology"],["dc.bibliographiccitation.lastpage","36"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Zeeshan, Nadia"],["dc.contributor.author","Naz, Saher"],["dc.contributor.author","Naz, Shumaila"],["dc.contributor.author","Afroz, Amber"],["dc.contributor.author","Zahur, Muzna"],["dc.contributor.author","Zia, Safia"],["dc.date.accessioned","2019-07-09T11:45:49Z"],["dc.date.available","2019-07-09T11:45:49Z"],["dc.date.issued","2018"],["dc.description.abstract","insubstantial amount for industrial applications. Endo-1, 4-β-glucanases (Egl) is one of the major enzyme involved in degradation of cellulose, an important component of plant cell wall. The present study was aimed at enhancing the production of endo-1, 4-β-glucanases (Egl) of Bacillus halodurans in Escherichia coli. Results: A putative Egl gene of Bacillus Halodurans was expressed in E. coli by cloning in pET 22b (+).On induction with isopropyl-b-D-1-thiogalactopyranoside, the enzyme expression reached upto ~20% of the cell protein producing 29.2 mg/liter culture. An increase in cell density to 12 in auto-inducing LB medium (absorbance at 600 nm) enhanced β-glucanase production up to 5.4 fold. The molecular mass of the enzyme was determined to be 39 KDa, which is nearly the same as the calculated value. Protein sequence was analyzed by CDD, Pfam, I TASSER, COACH, PROCHECK Servers and putative amino acids involved in the formation of catalytic, substrate and metal binding domains were identified. Phylogenetic analysis of the β-glucanases of B. halodurans was performed and position of Egl among other members of the genus Bacillus producing endo-glucanases was determined. Temperature and pH optima of the enzyme were found to be 60°C and 8.0, respectively, under the assay conditions. Conclusion: Production of endo-1, 4 β-glucanase enzymes fromB. halodurans increased several foldswhen cloned in pET vector and expressed in E. coli. To our knowledge, this is the first report of high-level expression and characterization of an endo-1, 4 β-glucanases from B. halodurans"],["dc.identifier.doi","10.1016/j.ejbt.2018.05.001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15322"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59315"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.subject.ddc","610"],["dc.title","Heterologous expression and enhanced production of β-1,4-glucanase of Bacillus halodurans C-125 in Escherichia coli"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
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