Cyganek, Lukas

[["dc.bibliographiccitation.firstpage","1928"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Europace"],["dc.bibliographiccitation.lastpage","1929"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Zhou, Xiaobo"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2020-12-10T18:19:09Z"],["dc.date.available","2020-12-10T18:19:09Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1093/europace/euz274"],["dc.identifier.eissn","1532-2092"],["dc.identifier.issn","1099-5129"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75144"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","‘Mature’ resting membrane potentials in hiPSC-CMs: fact or artefact?—Authors’ reply"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Stem Cells International"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","2018"],["dc.contributor.author","Zhao, Zhihan"],["dc.contributor.author","Lan, Huan"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Li, Xin"],["dc.contributor.author","Buljubasic, Fanis"],["dc.contributor.author","Sattler, Katherine"],["dc.contributor.author","Yücel, Gökhan"],["dc.contributor.author","Lang, Siegfried"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Utikal, Jochen"],["dc.contributor.author","Wieland, Thomas"],["dc.contributor.author","Borggrefe, Martin"],["dc.contributor.author","Zhou, Xiao-Bo"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2020-12-10T18:37:41Z"],["dc.date.available","2020-12-10T18:37:41Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1155/2018/6067096"],["dc.identifier.pmid","29535773"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77065"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/325"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","112"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Cardiovascular Development and Disease"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Xu, Qiang"],["dc.contributor.author","Huang, Xuemei"],["dc.contributor.author","Meng, Zenghui"],["dc.contributor.author","Li, Yingrui"],["dc.contributor.author","Zhong, Rujia"],["dc.contributor.author","Li, Xin"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Akin, Ibrahim"],["dc.contributor.author","Zhou, Xiaobo"],["dc.contributor.author","Lan, Huan"],["dc.date.accessioned","2022-05-02T08:09:37Z"],["dc.date.available","2022-05-02T08:09:37Z"],["dc.date.issued","2022"],["dc.description.abstract","(1) Background: Short QT syndrome (SQTS) may result in sudden cardiac death. So far, no drugs, except quinidine, have been demonstrated to be effective in some patients with SQTS type 1 (SQTS1). This study was designed to examine the potential effectiveness of vernakalant for treating SQTS1 patients, using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) from a patient with SQTS1. (2) Methods: Patch clamp and calcium imaging techniques were used to examine the drug effects. (3) Results: Vernakalant prolonged the action potential duration (APD) in hiPSC-CMs from a SQTS1-patient (SQTS1-hiPSC-CMs). In spontaneously beating SQTS1-hiPSC-CMs, vernakalant reduced the arrhythmia-like events induced by carbachol plus epinephrine. Vernakalant failed to suppress the hERG channel currents but reduced the outward small-conductance calcium-activated potassium channel current. In addition, it enhanced Na/Ca exchanger currents and late sodium currents, in agreement with its APD-prolonging effect. (4) Conclusions: The results demonstrated that vernakalant can prolong APD and reduce arrhythmia-like events in SQTS1-hiPSC-CMs and may be a candidate drug for treating arrhythmias in SQTS1-patients."],["dc.description.abstract","(1) Background: Short QT syndrome (SQTS) may result in sudden cardiac death. So far, no drugs, except quinidine, have been demonstrated to be effective in some patients with SQTS type 1 (SQTS1). This study was designed to examine the potential effectiveness of vernakalant for treating SQTS1 patients, using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) from a patient with SQTS1. (2) Methods: Patch clamp and calcium imaging techniques were used to examine the drug effects. (3) Results: Vernakalant prolonged the action potential duration (APD) in hiPSC-CMs from a SQTS1-patient (SQTS1-hiPSC-CMs). In spontaneously beating SQTS1-hiPSC-CMs, vernakalant reduced the arrhythmia-like events induced by carbachol plus epinephrine. Vernakalant failed to suppress the hERG channel currents but reduced the outward small-conductance calcium-activated potassium channel current. In addition, it enhanced Na/Ca exchanger currents and late sodium currents, in agreement with its APD-prolonging effect. (4) Conclusions: The results demonstrated that vernakalant can prolong APD and reduce arrhythmia-like events in SQTS1-hiPSC-CMs and may be a candidate drug for treating arrhythmias in SQTS1-patients."],["dc.identifier.doi","10.3390/jcdd9040112"],["dc.identifier.pii","jcdd9040112"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107421"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-561"],["dc.relation.eissn","2308-3425"],["dc.title","Antiarrhythmic Effects of Vernakalant in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes from a Patient with Short QT Syndrome Type 1"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1140"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Clinical Research in Cardiology"],["dc.bibliographiccitation.lastpage","1146"],["dc.bibliographiccitation.volume","108"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Besler, Johanna"],["dc.contributor.author","Ansari, Uzair"],["dc.contributor.author","Liebe, Volker"],["dc.contributor.author","Schimpf, Rainer"],["dc.contributor.author","Tülümen, Erol"],["dc.contributor.author","Rudic, Boris"],["dc.contributor.author","Lang, Siegfried"],["dc.contributor.author","Odening, Katja"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Wolpert, Christian"],["dc.contributor.author","Zhou, Xiaobo"],["dc.contributor.author","Borggrefe, Martin"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2020-12-10T14:10:22Z"],["dc.date.available","2020-12-10T14:10:22Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1007/s00392-019-01449-3"],["dc.identifier.eissn","1861-0692"],["dc.identifier.issn","1861-0684"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70740"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Long-term follow-up of implantable cardioverter-defibrillators in Short QT syndrome"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1410"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Europace"],["dc.bibliographiccitation.lastpage","1421"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Albers, Sebastian"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Zhao, Zhihan"],["dc.contributor.author","Lan, Huan"],["dc.contributor.author","Li, Xin"],["dc.contributor.author","Xu, Qiang"],["dc.contributor.author","Kleinsorge, Mandy"],["dc.contributor.author","Huang, Mengying"],["dc.contributor.author","Liao, Zhenxing"],["dc.contributor.author","Zhong, Rujia"],["dc.contributor.author","Rudic, Boris"],["dc.contributor.author","Müller, Jonas"],["dc.contributor.author","Dinkel, Hendrik"],["dc.contributor.author","Lang, Siegfried"],["dc.contributor.author","Diecke, Sebastian"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.contributor.author","Utikal, Jochen"],["dc.contributor.author","Wieland, Thomas"],["dc.contributor.author","Borggrefe, Martin"],["dc.contributor.author","Zhou, Xiaobo"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2020-12-10T18:19:09Z"],["dc.date.available","2020-12-10T18:19:09Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1093/europace/euz122"],["dc.identifier.pmid","31106349"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75142"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/328"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","A cellular model of Brugada syndrome with SCN10A variants using human-induced pluripotent stem cell-derived cardiomyocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Cardiovascular Medicine"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Huang, Mengying"],["dc.contributor.author","Yang, Zhen"],["dc.contributor.author","Li, Yingrui"],["dc.contributor.author","Lan, Huan"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Yuecel, Goekhan"],["dc.contributor.author","Lang, Siegfried"],["dc.contributor.author","Bieback, Karen"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Zhou, Xiaobo"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2022-04-01T10:01:10Z"],["dc.date.available","2022-04-01T10:01:10Z"],["dc.date.issued","2022"],["dc.description.abstract","Background Previous studies suggested involvement of non-ß-adrenoceptors in the pathogenesis of Takotsubo cardiomyopathy (TTC). This study was designed to explore possible roles and underlying mechanisms of dopamine D1/D5 receptor coupled signaling in arrhythmogenesis of TTC. Methods Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were challenged by toxic concentration of epinephrine (Epi, 0.5 mM for 1 h) for mimicking the catecholamine excess in setting of TTC. Specific receptor blockers and activators were used to unveil roles of D1/D5 receptors. Patch clamp, qPCR, and FACS analyses were performed in the study. Results High concentration Epi and two dopamine D1/D5 receptor agonists [(±)-SKF 38393 and fenoldopam] reduced the depolarization velocity and prolonged the duration of action potentials (APs) and caused arrhythmic events in iPSC-CMs, suggesting involvement of dopamine D1/D5 receptor signaling in arrhythmogenesis associated with QT interval prolongation in the setting of TTC. (±)-SKF 38393 and fenoldopam enhanced the reactive oxygen species (ROS)-production. H 2 O 2 (100 μM) recapitulated the effects of (±)-SKF 38393 and fenoldopam on APs and a ROS-blocker N -acetylcysteine (NAC, 1 mM) abolished the effects, suggesting that the ROS-signaling is involved in the dopamine D1/D5 receptor actions. A NADPH oxidases blocker and a PKA- or PKC-blocker suppressed the effects of the dopamine receptor agonist, implying that PKA, NADPH oxidases and PKC participated in dopamine D1/D5 receptor signaling. The abnormal APs resulted from dopamine D1/D5 receptor activation-induced dysfunctions of ion channels including the Na + and L-type Ca 2+ and I Kr channels. Conclusions Dopamine D1/D5 receptor signaling plays important roles for arrhythmogenesis of TTC. Dopamine D1/D5 receptor signaling in cardiomyocytes might be a potential target for treating arrhythmias in patients with TTC."],["dc.identifier.doi","10.3389/fcvm.2021.777463"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/105614"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation.eissn","2297-055X"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Dopamine D1/D5 Receptor Signaling Is Involved in Arrhythmogenesis in the Setting of Takotsubo Cardiomyopathy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","486"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Clinical Medicine"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Buljubasic, Fanis"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Lan, Huan"],["dc.contributor.author","Lomada, Santosh K."],["dc.contributor.author","Chatterjee, Anupriya"],["dc.contributor.author","Zhao, Zhihan"],["dc.contributor.author","Li, Xin"],["dc.contributor.author","Zhong, Rujia"],["dc.contributor.author","Xu, Qiang"],["dc.contributor.author","Huang, Mengying"],["dc.contributor.author","Liao, Zhenxing"],["dc.contributor.author","Lang, Siegfried"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Zhou, Xiaobo"],["dc.contributor.author","Wieland, Thomas"],["dc.contributor.author","Borggrefe, Martin"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2020-12-10T18:47:12Z"],["dc.date.available","2020-12-10T18:47:12Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/jcm9020486"],["dc.identifier.eissn","2077-0383"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78680"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Nucleoside Diphosphate Kinase B Contributes to Arrhythmogenesis in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes from a Patient with Arrhythmogenic Right Ventricular Cardiomyopathy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","195"],["dc.bibliographiccitation.journal","International Journal of Cardiology"],["dc.bibliographiccitation.lastpage","202"],["dc.bibliographiccitation.volume","254"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Zhao, Zhihan"],["dc.contributor.author","Lan, Huan"],["dc.contributor.author","Schünemann, Jan-Dierk"],["dc.contributor.author","Sattler, Katherine"],["dc.contributor.author","Buljubasic, Fanis"],["dc.contributor.author","Patocskai, Bence"],["dc.contributor.author","Li, Xin"],["dc.contributor.author","Yücel, Gökhan"],["dc.contributor.author","Lang, Siegfried"],["dc.contributor.author","Nowak, Daniel"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Bieback, Karen"],["dc.contributor.author","Utikal, Jochen"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.contributor.author","Ravens, Ursula"],["dc.contributor.author","Wieland, Thomas"],["dc.contributor.author","Borggrefe, Martin"],["dc.contributor.author","Zhou, Xiao-Bo"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2018-04-23T11:49:14Z"],["dc.date.available","2018-04-23T11:49:14Z"],["dc.date.issued","2018"],["dc.description.abstract","Background and purpose Previous studies revealed that Takotsubo cardiomyopathy (TTC), a transient disorder of ventricular dysfunction affecting predominantly postmenopausal women, is associated with acquired long QT syndrome and arrhythmias, but the exact pathophysiologic mechanism is unknown. Our aim is to investigate the electrophysiological mechanism for QT-prolongation in TTC-patients by using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Methods hiPSC-CMs, which were generated from human skin fibroblasts of three healthy donors, were treated by estradiol (10 μM for one week) and a toxic concentration of isoprenaline (Iso, 1 mM for 2 h). Patch clamp techniques, qPCR and fluorescence-activated cell sorting (FACS) were employed for the study. Key results Iso enhanced late INa and suppressed Ito and thus prolonged the action potential duration (APD), suggesting possible reasons for arrhythmias in TTC. Iso elevated the production of reactive oxygen species (ROS). N-acetylcystein (1 mM), a ROS-blocker, abolished the effects of Iso on late INa and Ito. H2O2 (100 μM) mimicked Iso effects on late INa and Ito. These data indicate that the effects of Iso were mediated by ROS. Metoprolol (1 mM), a beta-blocker, prevented the effects of Iso on late INa and APD, confirming the adrenoceptor-dependent effects of Iso. Estradiol treatment prevented the APD-prolongation, attenuated the enhancement of INa, diminished the reduction of Ito, suppressed ROS-production induced by Iso and reduced the expression levels of adrenoceptors, suggesting protective effects of estragon against toxic effects of catecholamine. Conclusions Estradiol has protective effects against catecholamine excess and hence reduction in estrogen level may increase the risk of acquired long QT syndrome in TTC."],["dc.identifier.doi","10.1016/j.ijcard.2017.11.007"],["dc.identifier.gro","3142511"],["dc.identifier.pmid","29407091"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13665"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/324"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","0167-5273"],["dc.title","Estradiol protection against toxic effects of catecholamine on electrical properties in human-induced pluripotent stem cell derived cardiomyocytes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","f46"],["dc.bibliographiccitation.issue","FI1"],["dc.bibliographiccitation.journal","Europace"],["dc.bibliographiccitation.lastpage","f56"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Zhao, Zhihan"],["dc.contributor.author","Lan, Huan"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Tombers, Christoph"],["dc.contributor.author","Li, Xin"],["dc.contributor.author","Buljubasic, Fanis"],["dc.contributor.author","Lang, Siegfried"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.contributor.author","Utikal, Jochen"],["dc.contributor.author","Wieland, Thomas"],["dc.contributor.author","Borggrefe, Martin"],["dc.contributor.author","Zhou, Xiao-Bo"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2020-12-10T18:19:08Z"],["dc.date.available","2020-12-10T18:19:08Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1093/europace/euy042"],["dc.identifier.pmid","29566126"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75139"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/271"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Electrical dysfunctions in human-induced pluripotent stem cell-derived cardiomyocytes from a patient with an arrhythmogenic right ventricular cardiomyopathy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","8507"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Fan, Xuehui"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Nitschke, Katja"],["dc.contributor.author","Uhlig, Stefanie"],["dc.contributor.author","Nuhn, Philipp"],["dc.contributor.author","Bieback, Karen"],["dc.contributor.author","Duerschmied, Daniel"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Zhou, Xiaobo"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2022-09-01T09:51:12Z"],["dc.date.available","2022-09-01T09:51:12Z"],["dc.date.issued","2022"],["dc.description.abstract","Endothelial cells derived from human induced pluripotent stem cells (hiPSC-ECs) provide a new opportunity for mechanistic research on vascular regeneration and drug screening. However, functions of hiPSC-ECs still need to be characterized. The objective of this study was to investigate electrophysiological and functional properties of hiPSC-ECs compared with primary human cardiac microvascular endothelial cells (HCMECs), mainly focusing on ion channels and membrane receptor signaling, as well as specific cell functions. HiPSC-ECs were derived from hiPS cells that were generated from human skin fibroblasts of three independent healthy donors. Phenotypic and functional comparison to HCMECs was performed by flow cytometry, immunofluorescence staining, quantitative reverse-transcription polymerase chain reaction (qPCR), enzyme-linked immunosorbent assay (ELISA), tube formation, LDL uptake, exosome release assays and, importantly, patch clamp techniques. HiPSC-ECs were successfully generated from hiPS cells and were identified by endothelial markers. The mRNA levels of KCNN2, KCNN4, KCNMA1, TRPV2, and SLC8A1 in hiPSC-ECs were significantly higher than HCMECs. AT1 receptor mRNA level in hiPSC-ECs was higher than in HCMECs. AT2 receptor mRNA level was the highest among all receptors. Adrenoceptor ADRA2 expression in hiPSC-ECs was lower than in HCMECs, while ADRA1, ADRB1, ADRB2, and G-protein GNA11 and Gai expression were similar in both cell types. The expression level of muscarinic and dopamine receptors CHRM3, DRD2, DRD3, and DRD4 in hiPSC-ECs were significantly lower than in HCMECs. The functional characteristics of endothelial cells, such as tube formation and LDL uptake assay, were not statistically different between hiPSC-ECs and HCMECs. Phenylephrine similarly increased the release of the vasoconstrictor endothelin-1 (ET-1) in hiPSC-ECs and HCMECs. Acetylcholine also similarly increased nitric oxide generation in hiPSC-ECs and HCMECs. The resting potentials (RPs), ISK1–3, ISK4 and IK1 were similar in hiPSC-ECs and HCMECs. IBK was larger and IKATP was smaller in hiPSC-ECs. In addition, we also noted a higher expression level of exosomes marker CD81 in hiPSC-ECs and a higher expression of CD9 and CD63 in HCMECs. However, the numbers of exosomes extracted from both types of cells did not differ significantly. The study demonstrates that hiPSC-ECs are similar to native endothelial cells in ion channel function and membrane receptor-coupled signaling and physiological cell functions, although some differences exist. This information may be helpful for research using hiPSC-ECs."],["dc.identifier.doi","10.3390/ijms23158507"],["dc.identifier.pii","ijms23158507"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113906"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","1422-0067"],["dc.title","Functional Characterization of Human Induced Pluripotent Stem Cell-Derived Endothelial Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]