Cyganek, Lukas

[["dc.contributor.author","Gönenc, Ipek Ilgin"],["dc.contributor.author","Wolff, Alexander"],["dc.contributor.author","Schmidt, Julia"],["dc.contributor.author","Zibat, Arne"],["dc.contributor.author","Müller, Christian"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Argyriou, Loukas"],["dc.contributor.author","Räschle, Markus"],["dc.contributor.author","Yigit, Gökhan"],["dc.contributor.author","Wollnik, Bernd"],["dc.date.accessioned","2022-02-23T16:37:14Z"],["dc.date.available","2022-02-23T16:37:14Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1101/2021.10.01.462717"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/100402"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/430"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Wollnik"],["dc.title","Single-cell transcription profiles in Bloom syndrome patients link BLM deficiency with altered condensin complex expression signatures"],["dc.type","preprint"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1137"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Europace"],["dc.bibliographiccitation.lastpage","1148"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Huang, Mengying"],["dc.contributor.author","Fan, Xuehui"],["dc.contributor.author","Yang, Zhen"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Li, Xin"],["dc.contributor.author","Yuecel, Goekhan"],["dc.contributor.author","Lan, Huan"],["dc.contributor.author","Li, Yingrui"],["dc.contributor.author","Wendel, Angela"],["dc.contributor.author","Lang, Siegfried"],["dc.contributor.author","Borggrefe, Martin"],["dc.date.accessioned","2021-10-01T09:57:54Z"],["dc.date.available","2021-10-01T09:57:54Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Aims This study aimed to investigate possible roles and underlying mechanisms of alpha-adrenoceptor coupled signalling for the pathogenesis of Takotsubo syndrome (TTS). Methods and results Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were treated with a toxic concentration of epinephrine (Epi, 0.5 mM for 1 h) to mimic the setting of TTS. Patch-clamp technique, polymerase chain reaction (PCR) and Fluorescence-activated cell sorting (FACS) were employed for the study. High concentration Epi suppressed the depolarization velocity, prolonged duration of action potentials and induced arrhythmic events in hiPSC-CMs. The Epi effects were attenuated by an alpha-adrenoceptor blocker (phentolamine), suggesting involvement of alpha-adrenoceptor signalling in arrhythmogenesis related to QT interval prolongation in the setting of TTS. An alpha 1-adrenoceptor agonist (phenylephrine) but not an alpha 2-adrenoceptor agonist (clonidine) mimicked Epi effects. Epi enhanced ROS production, which could be attenuated by the alpha- adrenoceptor blocker. Treatment of cells with H2O2 (100 µM) mimicked the effects of Epi on action potentials and a reactive oxygen species (ROS)-blocker (N-acetyl-I-cysteine, 1 mM) prevented the Epi effects, indicating that the ROS signalling is involved in the alpha-adrenoceptor actions. Nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidases were involved in alpha 1-adrenoceptor signalling. A protein kinase C (PKC) blocker suppressed the effects of Epi, phenylephrine and ROS as well, implying that PKC participated in alpha 1-adrenoceptor signalling and acted as a downstream factor of ROS. The abnormal action potentials resulted from alpha 1-adrenoceptor activation-induced dysfunctions of ion channels including the voltage-dependent Na+ and L-type Ca2+ channels. Conclusions Alpha 1-adrenoceptor signalling plays important roles for arrhythmogenesis of TTS. Alpha-adrenoceptor blockers might be clinically helpful for treating arrhythmias in patients with TTS."],["dc.identifier.doi","10.1093/europace/euab008"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89942"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-469"],["dc.relation.eissn","1532-2092"],["dc.relation.issn","1099-5129"],["dc.title","Alpha 1-adrenoceptor signalling contributes to toxic effects of catecholamine on electrical properties in cardiomyocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","527"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Lutter, Georg"],["dc.contributor.author","Puehler, Thomas"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Seiler, Jette"],["dc.contributor.author","Rogler, Anita"],["dc.contributor.author","Herberth, Tanja"],["dc.contributor.author","Knueppel, Philipp"],["dc.contributor.author","Gorb, Stanislav N."],["dc.contributor.author","Sathananthan, Janarthanan"],["dc.contributor.author","Sellers, Stephanie"],["dc.contributor.author","Haben, Irma"],["dc.date.accessioned","2022-04-01T10:03:18Z"],["dc.date.available","2022-04-01T10:03:18Z"],["dc.date.issued","2022"],["dc.description.abstract","Clinically used heart valve prostheses, despite their progress, are still associated with limitations. Biodegradable poly-ε-caprolactone (PCL) nanofiber scaffolds, as a matrix, were seeded with human endothelial colony-forming cells (ECFCs) and human induced-pluripotent stem cells-derived MSCs (iMSCs) for the generation of tissue-engineered heart valves. Cell adhesion, proliferation, and distribution, as well as the effects of coating PCL nanofibers, were analyzed by fluorescence microscopy and SEM. Mechanical properties of seeded PCL scaffolds were investigated under uniaxial loading. iPSCs were used to differentiate into iMSCs via mesoderm. The obtained iMSCs exhibited a comparable phenotype and surface marker expression to adult human MSCs and were capable of multilineage differentiation. EFCFs and MSCs showed good adhesion and distribution on PCL fibers, forming a closed cell cover. Coating of the fibers resulted in an increased cell number only at an early time point; from day 7 of colonization, there was no difference between cell numbers on coated and uncoated PCL fibers. The mechanical properties of PCL scaffolds under uniaxial loading were compared with native porcine pulmonary valve leaflets. The Young’s modulus and mean elongation at Fmax of unseeded PCL scaffolds were comparable to those of native leaflets (p = ns.). Colonization of PCL scaffolds with human ECFCs or iMSCs did not alter these properties (p = ns.). However, the native heart valves exhibited a maximum tensile stress at a force of 1.2 ± 0.5 N, whereas it was lower in the unseeded PCL scaffolds (0.6 ± 0.0 N, p < 0.05). A closed cell layer on PCL tissues did not change the values of Fmax (ECFCs: 0.6 ± 0.1 N; iMSCs: 0.7 ± 0.1 N). Here, a successful two-phase protocol, based on the timed use of differentiation factors for efficient differentiation of human iPSCs into iMSCs, was developed. Furthermore, we demonstrated the successful colonization of a biodegradable PCL nanofiber matrix with human ECFCs and iMSCs suitable for the generation of tissue-engineered heart valves. A closed cell cover was already evident after 14 days for ECFCs and 21 days for MSCs. The PCL tissue did not show major mechanical differences compared to native heart valves, which was not altered by short-term surface colonization with human cells in the absence of an extracellular matrix."],["dc.identifier.doi","10.3390/ijms23010527"],["dc.identifier.pii","ijms23010527"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106133"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation.eissn","1422-0067"],["dc.title","Biodegradable Poly-ε-Caprolactone Scaffolds with ECFCs and iMSCs for Tissue-Engineered Heart Valves"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","844441"],["dc.bibliographiccitation.journal","Frontiers in Cardiovascular Medicine"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Jakobi, Tobias"],["dc.contributor.author","Groß, Julia"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Doroudgar, Shirin"],["dc.date.accessioned","2022-07-01T07:35:28Z"],["dc.date.available","2022-07-01T07:35:28Z"],["dc.date.issued","2022"],["dc.description.abstract","Introduction Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease (COVID-19) has emerged as a major cause of morbidity and mortality worldwide, placing unprecedented pressure on healthcare. Cardiomyopathy is described in patients with severe COVID-19 and increasing evidence suggests that cardiovascular involvement portends a high mortality. To facilitate fast development of antiviral interventions, drugs initially developed to treat other diseases are currently being repurposed as COVID-19 treatments. While it has been shown that SARS-CoV-2 invades cells through the angiotensin-converting enzyme 2 receptor (ACE2), the effect of drugs currently repurposed to treat COVID-19 on the heart requires further investigation. Methods Human induced pluripotent stem cell-derived cardiac myocytes (hiPSC-CMs) were treated with five repurposed drugs (remdesivir, lopinavir/ritonavir, lopinavir/ritonavir/interferon beta (INF-β), hydroxychloroquine, and chloroquine) and compared with DMSO controls. Transcriptional profiling was performed to identify global changes in gene expression programs. Results RNA sequencing of hiPSC-CMs revealed significant changes in gene programs related to calcium handling and the endoplasmic reticulum stress response, most prominently for lopinavir/ritonavir and lopinavir/ritonavir/interferon-beta. The results of the differential gene expression analysis are available for interactive access at https://covid19drugs.jakobilab.org . Conclusion Transcriptional profiling in hiPSC-CMs treated with COVID-19 drugs identified unfavorable changes with lopinavir/ritonavir and lopinavir/ritonavir/INF-β in key cardiac gene programs that may negatively affect heart function."],["dc.identifier.doi","10.3389/fcvm.2022.844441"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112179"],["dc.notes.intern","DOI-Import GROB-581"],["dc.relation.eissn","2297-055X"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Transcriptional Effects of Candidate COVID-19 Treatments on Cardiac Myocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1928"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Europace"],["dc.bibliographiccitation.lastpage","1929"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Zhou, Xiaobo"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2020-12-10T18:19:09Z"],["dc.date.available","2020-12-10T18:19:09Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1093/europace/euz274"],["dc.identifier.eissn","1532-2092"],["dc.identifier.issn","1099-5129"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75144"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","‘Mature’ resting membrane potentials in hiPSC-CMs: fact or artefact?—Authors’ reply"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Stem Cells International"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","2018"],["dc.contributor.author","Zhao, Zhihan"],["dc.contributor.author","Lan, Huan"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Li, Xin"],["dc.contributor.author","Buljubasic, Fanis"],["dc.contributor.author","Sattler, Katherine"],["dc.contributor.author","Yücel, Gökhan"],["dc.contributor.author","Lang, Siegfried"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Utikal, Jochen"],["dc.contributor.author","Wieland, Thomas"],["dc.contributor.author","Borggrefe, Martin"],["dc.contributor.author","Zhou, Xiao-Bo"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2020-12-10T18:37:41Z"],["dc.date.available","2020-12-10T18:37:41Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1155/2018/6067096"],["dc.identifier.pmid","29535773"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77065"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/325"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Cell and Developmental Biology"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Li, Wener"],["dc.contributor.author","Stauske, Michael"],["dc.contributor.author","Luo, Xiaojing"],["dc.contributor.author","Wagner, Stefan"],["dc.contributor.author","Vollrath, Meike"],["dc.contributor.author","Mehnert, Carola S."],["dc.contributor.author","Schubert, Mario"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Chen, Simin"],["dc.contributor.author","Hasheminasab, Sayed-Mohammad"],["dc.contributor.author","Wulf, Gerald"],["dc.contributor.author","El-Armouche, Ali"],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Hasenfuss, Gerd"],["dc.contributor.author","Guan, Kaomei"],["dc.date.accessioned","2021-04-14T08:31:12Z"],["dc.date.available","2021-04-14T08:31:12Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3389/fcell.2020.592893"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83516"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2296-634X"],["dc.title","Disease Phenotypes and Mechanisms of iPSC-Derived Cardiomyocytes From Brugada Syndrome Patients With a Loss-of-Function SCN5A Mutation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","112"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Cardiovascular Development and Disease"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Xu, Qiang"],["dc.contributor.author","Huang, Xuemei"],["dc.contributor.author","Meng, Zenghui"],["dc.contributor.author","Li, Yingrui"],["dc.contributor.author","Zhong, Rujia"],["dc.contributor.author","Li, Xin"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Akin, Ibrahim"],["dc.contributor.author","Zhou, Xiaobo"],["dc.contributor.author","Lan, Huan"],["dc.date.accessioned","2022-05-02T08:09:37Z"],["dc.date.available","2022-05-02T08:09:37Z"],["dc.date.issued","2022"],["dc.description.abstract","(1) Background: Short QT syndrome (SQTS) may result in sudden cardiac death. So far, no drugs, except quinidine, have been demonstrated to be effective in some patients with SQTS type 1 (SQTS1). This study was designed to examine the potential effectiveness of vernakalant for treating SQTS1 patients, using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) from a patient with SQTS1. (2) Methods: Patch clamp and calcium imaging techniques were used to examine the drug effects. (3) Results: Vernakalant prolonged the action potential duration (APD) in hiPSC-CMs from a SQTS1-patient (SQTS1-hiPSC-CMs). In spontaneously beating SQTS1-hiPSC-CMs, vernakalant reduced the arrhythmia-like events induced by carbachol plus epinephrine. Vernakalant failed to suppress the hERG channel currents but reduced the outward small-conductance calcium-activated potassium channel current. In addition, it enhanced Na/Ca exchanger currents and late sodium currents, in agreement with its APD-prolonging effect. (4) Conclusions: The results demonstrated that vernakalant can prolong APD and reduce arrhythmia-like events in SQTS1-hiPSC-CMs and may be a candidate drug for treating arrhythmias in SQTS1-patients."],["dc.description.abstract","(1) Background: Short QT syndrome (SQTS) may result in sudden cardiac death. So far, no drugs, except quinidine, have been demonstrated to be effective in some patients with SQTS type 1 (SQTS1). This study was designed to examine the potential effectiveness of vernakalant for treating SQTS1 patients, using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) from a patient with SQTS1. (2) Methods: Patch clamp and calcium imaging techniques were used to examine the drug effects. (3) Results: Vernakalant prolonged the action potential duration (APD) in hiPSC-CMs from a SQTS1-patient (SQTS1-hiPSC-CMs). In spontaneously beating SQTS1-hiPSC-CMs, vernakalant reduced the arrhythmia-like events induced by carbachol plus epinephrine. Vernakalant failed to suppress the hERG channel currents but reduced the outward small-conductance calcium-activated potassium channel current. In addition, it enhanced Na/Ca exchanger currents and late sodium currents, in agreement with its APD-prolonging effect. (4) Conclusions: The results demonstrated that vernakalant can prolong APD and reduce arrhythmia-like events in SQTS1-hiPSC-CMs and may be a candidate drug for treating arrhythmias in SQTS1-patients."],["dc.identifier.doi","10.3390/jcdd9040112"],["dc.identifier.pii","jcdd9040112"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107421"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-561"],["dc.relation.eissn","2308-3425"],["dc.title","Antiarrhythmic Effects of Vernakalant in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes from a Patient with Short QT Syndrome Type 1"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1140"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Clinical Research in Cardiology"],["dc.bibliographiccitation.lastpage","1146"],["dc.bibliographiccitation.volume","108"],["dc.contributor.author","El-Battrawy, Ibrahim"],["dc.contributor.author","Besler, Johanna"],["dc.contributor.author","Ansari, Uzair"],["dc.contributor.author","Liebe, Volker"],["dc.contributor.author","Schimpf, Rainer"],["dc.contributor.author","Tülümen, Erol"],["dc.contributor.author","Rudic, Boris"],["dc.contributor.author","Lang, Siegfried"],["dc.contributor.author","Odening, Katja"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Wolpert, Christian"],["dc.contributor.author","Zhou, Xiaobo"],["dc.contributor.author","Borggrefe, Martin"],["dc.contributor.author","Akin, Ibrahim"],["dc.date.accessioned","2020-12-10T14:10:22Z"],["dc.date.available","2020-12-10T14:10:22Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1007/s00392-019-01449-3"],["dc.identifier.eissn","1861-0692"],["dc.identifier.issn","1861-0684"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70740"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Long-term follow-up of implantable cardioverter-defibrillators in Short QT syndrome"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Grunert, Marcel"],["dc.contributor.author","Appelt, Sandra"],["dc.contributor.author","Schönhals, Sophia"],["dc.contributor.author","Mika, Kerstin"],["dc.contributor.author","Cui, Huanhuan"],["dc.contributor.author","Cooper, Ashley"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Sperling, Silke R."],["dc.date.accessioned","2021-04-14T08:24:27Z"],["dc.date.available","2021-04-14T08:24:27Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1038/s41598-020-67872-z"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81289"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2045-2322"],["dc.title","Induced pluripotent stem cells of patients with Tetralogy of Fallot reveal transcriptional alterations in cardiomyocyte differentiation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]