Batschkus, Sarah

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Case Reports in Dentistry"],["dc.bibliographiccitation.lastpage","8"],["dc.bibliographiccitation.volume","2018"],["dc.contributor.author","Santander, P."],["dc.contributor.author","Schwaibold, E. M. C."],["dc.contributor.author","Bremmer, F."],["dc.contributor.author","Batschkus, S."],["dc.contributor.author","Kauffmann, P."],["dc.date.accessioned","2019-07-09T11:45:55Z"],["dc.date.available","2019-07-09T11:45:55Z"],["dc.date.issued","2018"],["dc.description.abstract","We report a case of multiple keratocysts first diagnosed in an 8-year-old boy. Case report. The incidental radiographic finding of a cystic lesion in an 8-year-old boy led to the surgical enucleation and further diagnosis of a keratocyst associated with a tooth crown. In the course of dental maturation from deciduous to permanent teeth, the boy presented new lesions, always associated with the crowns of teeth. Gorlin-Goltz (nevoid basal-cell carcinoma) syndrome was suspected, and the genetic analysis detected a previously undescribed germline variant in the PTCH1 gene. Treatment. This included a surgical removal of the cystic lesions, as well as the affected teeth. Follow-up. Due to the high recurrence rate of the keratocysts, frequent radiological checks were performed over a 5-year period."],["dc.identifier.doi","10.1155/2018/7594840"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15343"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59336"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2090-6455"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Multiple, Multiloculated, and Recurrent Keratocysts of the Mandible and Maxilla in Association with Gorlin-Goltz (Nevoid Basal-Cell Carcinoma) Syndrome: A Pediatric Case Report and Follow-up over 5 Years"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","14751"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific reports"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Kirschneck, Christian"],["dc.contributor.author","Batschkus, Sarah"],["dc.contributor.author","Proff, Peter"],["dc.contributor.author","Köstler, Josef"],["dc.contributor.author","Spanier, Gerrit"],["dc.contributor.author","Schröder, Agnes"],["dc.date.accessioned","2019-07-09T11:44:39Z"],["dc.date.available","2019-07-09T11:44:39Z"],["dc.date.issued","2017-11-07"],["dc.description.abstract","Meaningful, reliable and valid mRNA expression analyses by real-time quantitative PCR (RT-qPCR) can only be achieved, if suitable reference genes are chosen for normalization and if appropriate RT-qPCR quality standards are met. Human periodontal ligament (hPDL) fibroblasts play a major mediating role in orthodontic tooth movement and periodontitis. Despite corresponding in-vitro gene expression studies being a focus of interest for many years, no information is available for hPDL fibroblasts on suitable reference genes, which are generally used in RT-qPCR experiments to normalize variability between samples. The aim of this study was to identify and validate suitable reference genes for normalization in untreated hPDL fibroblasts as well as experiments on orthodontic tooth movement or periodontitis (Aggregatibacter actinomycetemcomitans). We investigated the suitability of 13 candidate reference genes using four different algorithms (geNorm, NormFinder, comparative ΔCq and BestKeeper) and ranked them according to their expression stability. Overall PPIB (peptidylprolyl isomerase A), TBP (TATA-box-binding protein) and RPL22 (ribosomal protein 22) were found to be most stably expressed with two genes in conjunction sufficient for reliable normalization. This study provides an accurate tool for quantitative gene expression analysis in hPDL fibroblasts according to the MIQE guidelines and shows that reference gene reliability is treatment-specific."],["dc.identifier.doi","10.1038/s41598-017-15281-0"],["dc.identifier.pmid","29116140"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14851"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59060"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/14855 but duplicate"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Valid gene expression normalization by RT-qPCR in studies on hPDL fibroblasts with focus on orthodontic tooth movement and periodontitis."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]