Shomroni, Orr

[["dc.bibliographiccitation.artnumber","4091"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Shomroni, Orr"],["dc.contributor.author","Sitte, Maren"],["dc.contributor.author","Schmidt, Julia"],["dc.contributor.author","Parbin, Sabnam"],["dc.contributor.author","Ludewig, Fabian"],["dc.contributor.author","Yigit, Gökhan"],["dc.contributor.author","Zelarayan, Laura Cecilia"],["dc.contributor.author","Streckfuss-Bömeke, Katrin"],["dc.contributor.author","Wollnik, Bernd"],["dc.contributor.author","Salinas, Gabriella"],["dc.date.accessioned","2022-04-01T10:01:43Z"],["dc.date.available","2022-04-01T10:01:43Z"],["dc.date.issued","2022"],["dc.description.abstract","Single cell multi-omics analysis has the potential to yield a comprehensive understanding of the cellular events that underlie the basis of human diseases. The cardinal feature to access this information is the technology used for single-cell isolation, barcoding, and sequencing. Most currently used single-cell RNA-sequencing platforms have limitations in several areas including cell selection, documentation and library chemistry. In this study, we describe a novel high-throughput, full-length, single-cell RNA-sequencing approach that combines the CellenONE isolation and sorting system with the ICELL8 processing instrument. This method offers substantial improvements in single cell selection, documentation and capturing rate. Moreover, it allows the use of flexible chemistry for library preparations and the analysis of living or fixed cells, whole cells independent of sizing and morphology, as well as of nuclei. We applied this method to dermal fibroblasts derived from six patients with different segmental progeria syndromes and defined phenotype associated pathway signatures with variant associated expression modifiers. These results validate the applicability of our method to highlight genotype-expression relationships for molecular phenotyping of individual cells derived from human patients."],["dc.description.sponsorship","Georg-August-Universität Göttingen"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.1038/s41598-022-07874-1"],["dc.identifier.pii","7874"],["dc.identifier.pmid","35260714"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/105735"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/460"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/424"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation.eissn","2045-2322"],["dc.relation.workinggroup","RG Wollnik"],["dc.relation.workinggroup","RG Zelarayán-Behrend (Developmental Pharmacology)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","A novel single-cell RNA-sequencing approach and its applicability connecting genotype to phenotype in ageing disease"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","160090"],["dc.bibliographiccitation.journal","Scientific Data"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Centeno, Tonatiuh Pena"],["dc.contributor.author","Shomroni, Orr"],["dc.contributor.author","Hennion, Magali"],["dc.contributor.author","Halder, Rashi"],["dc.contributor.author","Vidal, Ramon"],["dc.contributor.author","Rahman, Raza-Ur"],["dc.contributor.author","Bonn, Stefan"],["dc.date.accessioned","2017-09-07T11:52:23Z"],["dc.date.available","2017-09-07T11:52:23Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1038/sdata.2016.90"],["dc.identifier.gro","3144912"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14127"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2588"],["dc.notes.intern","Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","public"],["dc.relation.issn","2052-4463"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Genome-wide chromatin and gene expression profiling during memory formation and maintenance in adult mice"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Foth, Rudi"],["dc.contributor.author","Shomroni, Orr"],["dc.contributor.author","Sigler, Matthias"],["dc.contributor.author","Hörer, Jürgen"],["dc.contributor.author","Cleuziou, Julie"],["dc.contributor.author","Paul, Thomas"],["dc.contributor.author","Eildermann, Katja"],["dc.date.accessioned","2021-04-14T08:30:16Z"],["dc.date.available","2021-04-14T08:30:16Z"],["dc.date.issued","2021"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1038/s41598-021-81340-2"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83174"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.title","Screening for potential targets to reduce stenosis in bioprosthetic heart valves"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Plant Physiology"],["dc.contributor.author","Krawczyk, Hannah Elisa"],["dc.contributor.author","Rotsch, Alexander Helmut"],["dc.contributor.author","Herrfurth, Cornelia"],["dc.contributor.author","Scholz, Patricia"],["dc.contributor.author","Shomroni, Orr"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Ischebeck, Till"],["dc.date.accessioned","2022-04-01T10:01:51Z"],["dc.date.available","2022-04-01T10:01:51Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract After reaching the stigma, pollen grains germinate and form a pollen tube that transports the sperm cells to the ovule. Due to selection pressure between pollen tubes, pollen grains likely evolved mechanisms to quickly adapt to temperature changes to sustain elongation at the highest possible rate. We investigated these adaptions in tobacco (Nicotiana tabacum) pollen tubes grown in vitro under 22°C and 37°C by a multi-omics approach including lipidomic, metabolomic, and transcriptomic analysis. Both glycerophospholipids and galactoglycerolipids increased in saturated acyl chains under heat stress (HS), while triacylglycerols (TGs) changed less in respect to desaturation but increased in abundance. Free sterol composition was altered, and sterol ester levels decreased. The levels of sterylglycosides and several sphingolipid classes and species were augmented. Most amino acid levels increased during HS, including the noncodogenic amino acids γ-amino butyrate and pipecolate. Furthermore, the sugars sedoheptulose and sucrose showed higher levels. Also, the transcriptome underwent pronounced changes with 1,570 of 24,013 genes being differentially upregulated and 813 being downregulated. Transcripts coding for heat shock proteins and many transcriptional regulators were most strongly upregulated but also transcripts that have so far not been linked to HS. Transcripts involved in TG synthesis increased, while the modulation of acyl chain desaturation seemed not to be transcriptionally controlled, indicating other means of regulation. In conclusion, we show that tobacco pollen tubes are able to rapidly remodel their lipidome under HS likely by post-transcriptional and/or post-translational regulation."],["dc.identifier.doi","10.1093/plphys/kiac127"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/105763"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation.eissn","1532-2548"],["dc.title","Heat stress leads to rapid lipid remodeling and transcriptional adaptations in Nicotiana tabacum pollen tubes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","24"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Schoger, Eric"],["dc.contributor.author","Carroll, Kelli J."],["dc.contributor.author","Iyer, Lavanya M."],["dc.contributor.author","McAnally, John R."],["dc.contributor.author","Tan, Wei"],["dc.contributor.author","Liu, Ning"],["dc.contributor.author","Noack, Claudia"],["dc.contributor.author","Shomroni, Orr"],["dc.contributor.author","Salinas, Gabriela"],["dc.contributor.author","Groß, Julia"],["dc.contributor.author","Herzog, Nicole"],["dc.contributor.author","Doroudgar, Shirin"],["dc.contributor.author","Bassel-Duby, Rhonda"],["dc.contributor.author","Zimmermann, Wolfram-H."],["dc.contributor.author","Zelarayán, Laura C."],["dc.date.accessioned","2020-12-10T18:38:00Z"],["dc.date.available","2020-12-10T18:38:00Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1161/CIRCRESAHA.118.314522"],["dc.identifier.pmid","31730408"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77162"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/332"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C04: Fibroblasten-Kardiomyozyten Interaktion im gesunden und erkrankten Herzen: Mechanismen und therapeutische Interventionen bei Kardiofibroblastopathien"],["dc.relation","SFB 1002 | C07: Kardiomyozyten Wnt/β-catenin Komplex Aktivität im pathologischen Herz-Remodeling - als gewebespezifischer therapeutischer Ansatz"],["dc.relation","SFB 1002 | S01: In vivo und in vitro Krankheitsmodelle"],["dc.relation.workinggroup","RG Zelarayán-Behrend (Developmental Pharmacology)"],["dc.relation.workinggroup","RG Zimmermann (Engineered Human Myocardium)"],["dc.title","CRISPR-Mediated Activation of Endogenous Gene Expression in the Postnatal Heart"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","S0166445X21001867"],["dc.bibliographiccitation.firstpage","105927"],["dc.bibliographiccitation.journal","Aquatic toxicology"],["dc.bibliographiccitation.volume","238"],["dc.contributor.author","Pfaff, Julia"],["dc.contributor.author","Reinwald, Hannes"],["dc.contributor.author","Ayobahan, Steve U."],["dc.contributor.author","Alvincz, Julia"],["dc.contributor.author","Göckener, Bernd"],["dc.contributor.author","Shomroni, Orr"],["dc.contributor.author","Salinas, Gabriela"],["dc.contributor.author","Düring, Rolf-Alexander"],["dc.contributor.author","Schäfers, Christoph"],["dc.contributor.author","Eilebrecht, Sebastian"],["dc.date.accessioned","2021-10-01T09:57:27Z"],["dc.date.available","2021-10-01T09:57:27Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.aquatox.2021.105927"],["dc.identifier.pii","S0166445X21001867"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89843"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-469"],["dc.relation.issn","0166-445X"],["dc.title","Toxicogenomic differentiation of functional responses to fipronil and imidacloprid in Daphnia magna"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","67"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Endocrinology"],["dc.bibliographiccitation.lastpage","79"],["dc.bibliographiccitation.volume","250"],["dc.contributor.author","Neumann, Anne-Marie"],["dc.contributor.author","Geißler, Cathleen"],["dc.contributor.author","Pilorz, Violetta"],["dc.contributor.author","Olejniczak, Iwona"],["dc.contributor.author","Lewis, Alfor G"],["dc.contributor.author","Seeley, Randy J"],["dc.contributor.author","Shomroni, Orr"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Kirchner, Henriette"],["dc.contributor.author","Oster, Henrik"],["dc.date.accessioned","2021-10-01T09:58:15Z"],["dc.date.available","2021-10-01T09:58:15Z"],["dc.date.issued","2021"],["dc.description.abstract","Bariatric surgery is still the most effective long-term weight-loss therapy. Recent data indicate that surgical outcomes may be affected by diurnal food intake patterns. In this study, we aimed to investigate how surgery-induced metabolic adaptations (i.e. weight loss) interact with circadian clock function. For that reason, vertical sleeve gastrectomy (VSG) was performed in obese mice and rhythms in behavior, tissue rhythmicity, and white adipose tissue transcriptome were evaluated. VSG under constant darkness conditions led to a maximum weight loss of 18% compared to a loss of 3% after sham surgery. Post-surgical weight development was characterized by two distinct intervals of catabolic and subsequent anabolic metabolic state. Locomotor activity was not affected. However, VSG significantly increased active phase meal frequency in the anabolic state. No significant effects on clock gene rhythmicity were detected in adrenal and white adipose tissue (WAT) explant cultures. Transcriptome rhythm analyses of subcutaneous WAT revealed a reduction of cycling genes after VSG (sham: 2493 vs VSG: 1013) independent of sustained rhythms in core clock gene expression. This may be a consequence of weight loss-induced morphological reconstruction of WAT that overwrites the direct influence of the local clock machinery on the transcriptome. However, VSG altered rhythmic transcriptional regulation of WAT lipid metabolism pathways. Thus, our data suggest a reorganization of diurnal metabolic rhythms after VSG downstream of the molecular clock machinery."],["dc.description.abstract","Bariatric surgery is still the most effective long-term weight-loss therapy. Recent data indicate that surgical outcomes may be affected by diurnal food intake patterns. In this study, we aimed to investigate how surgery-induced metabolic adaptations (i.e. weight loss) interact with circadian clock function. For that reason, vertical sleeve gastrectomy (VSG) was performed in obese mice and rhythms in behavior, tissue rhythmicity, and white adipose tissue transcriptome were evaluated. VSG under constant darkness conditions led to a maximum weight loss of 18% compared to a loss of 3% after sham surgery. Post-surgical weight development was characterized by two distinct intervals of catabolic and subsequent anabolic metabolic state. Locomotor activity was not affected. However, VSG significantly increased active phase meal frequency in the anabolic state. No significant effects on clock gene rhythmicity were detected in adrenal and white adipose tissue (WAT) explant cultures. Transcriptome rhythm analyses of subcutaneous WAT revealed a reduction of cycling genes after VSG (sham: 2493 vs VSG: 1013) independent of sustained rhythms in core clock gene expression. This may be a consequence of weight loss-induced morphological reconstruction of WAT that overwrites the direct influence of the local clock machinery on the transcriptome. However, VSG altered rhythmic transcriptional regulation of WAT lipid metabolism pathways. Thus, our data suggest a reorganization of diurnal metabolic rhythms after VSG downstream of the molecular clock machinery."],["dc.identifier.doi","10.1530/JOE-20-0611"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/90024"],["dc.notes.intern","DOI Import GROB-469"],["dc.relation.eissn","1479-6805"],["dc.relation.issn","0022-0795"],["dc.title","Restructuring of the male mice peripheral circadian network after bariatric surgery"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2740"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Cell Death and Differentiation"],["dc.bibliographiccitation.lastpage","2757"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Wildung, Merit"],["dc.contributor.author","Esser, Tilman Uli"],["dc.contributor.author","Grausam, Katie Baker"],["dc.contributor.author","Wiedwald, Cornelia"],["dc.contributor.author","Volceanov-Hahn, Larisa"],["dc.contributor.author","Riedel, Dietmar"],["dc.contributor.author","Beuermann, Sabine"],["dc.contributor.author","Li, Li"],["dc.contributor.author","Zylla, Jessica"],["dc.contributor.author","Guenther, Ann-Kathrin"],["dc.contributor.author","Wienken, Magdalena"],["dc.contributor.author","Ercetin, Evrim"],["dc.contributor.author","Han, Zhiyuan"],["dc.contributor.author","Bremmer, Felix"],["dc.contributor.author","Shomroni, Orr"],["dc.contributor.author","Andreas, Stefan"],["dc.contributor.author","Zhao, Haotian"],["dc.contributor.author","Lizé, Muriel"],["dc.date.accessioned","2020-12-10T18:09:42Z"],["dc.date.available","2020-12-10T18:09:42Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1038/s41418-019-0332-7"],["dc.identifier.eissn","1476-5403"],["dc.identifier.issn","1350-9047"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73732"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Transcription factor TAp73 and microRNA-449 complement each other to support multiciliogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","647"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Neurochemistry"],["dc.bibliographiccitation.lastpage","661"],["dc.bibliographiccitation.volume","154"],["dc.contributor.author","Ripamonti, Silvia"],["dc.contributor.author","Shomroni, Orr"],["dc.contributor.author","Rhee, Jeong Seop"],["dc.contributor.author","Chowdhury, Kamal"],["dc.contributor.author","Jahn, Olaf"],["dc.contributor.author","Hellmann, Klaus Peter"],["dc.contributor.author","Bonn, Stefan"],["dc.contributor.author","Brose, Nils"],["dc.contributor.author","Tirard, Marilyn"],["dc.date.accessioned","2021-04-14T08:26:35Z"],["dc.date.available","2021-04-14T08:26:35Z"],["dc.date.issued","2020"],["dc.description.abstract","SUMOylation is a dynamic post-translational protein modification that primarily takes place in cell nuclei, where it plays a key role in multiple DNA-related processes. In neurons, the SUMOylation-dependent control of a subset of neuronal transcription factors is known to regulate various aspects of nerve cell differentiation, development, and function. In an unbiased screen for endogenous SUMOylation targets in the developing mouse brain, based on a His6-HA-SUMO1 knock-in mouse line, we previously identified the transcription factor Zinc finger and BTB domain-containing 20 (Zbtb20) as a new SUMO1-conjugate. We show here that the three key SUMO paralogues SUMO1, SUMO2, and SUMO3 can all be conjugated to Zbtb20 in vitro in HEK293FT cells, and we confirm the SUMOylation of Zbtb20 in vivo in mouse brain. Using primary hippocampal neurons from wild-type and Zbtb20 knock-out (KO) mice as a model system, we then demonstrate that the expression of Zbtb20 is required for proper nerve cell development and neurite growth and branching. Furthermore, we show that the SUMOylation of Zbtb20 is essential for its function in this context, and provide evidence indicating that SUMOylation affects the Zbtb20-dependent transcriptional profile of neurons. Our data highlight the role of SUMOylation in the regulation of neuronal transcription factors that determine nerve cell development, and they demonstrate that key functions of the transcription factor Zbtb20 in neuronal development and neurite growth are under obligatory SUMOylation control."],["dc.identifier.doi","10.1111/jnc.15008"],["dc.identifier.pmid","32233089"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82001"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/73"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A09: SUMOylation und Neddylation in Synapsen"],["dc.relation","SFB 1286 | Z02: Integrative Datenanalyse und -interpretation. Generierung einer synaptisch-integrativen Datenstrategie (SynIDs)"],["dc.relation.eissn","1471-4159"],["dc.relation.issn","0022-3042"],["dc.relation.workinggroup","RG Bonn"],["dc.relation.workinggroup","RG Brose"],["dc.rights","CC BY 4.0"],["dc.title","SUMOylation controls the neurodevelopmental function of the transcription factor Zbtb20"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1689"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Journal of Molecular Medicine"],["dc.bibliographiccitation.lastpage","1700"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Pabel, Steffen"],["dc.contributor.author","Reetz, Florian"],["dc.contributor.author","Dybkova, Nataliya"],["dc.contributor.author","Shomroni, Orr"],["dc.contributor.author","Salinas, Gabriela"],["dc.contributor.author","Mustroph, Julian"],["dc.contributor.author","Hammer, Karin P."],["dc.contributor.author","Hasenfuss, Gerd"],["dc.contributor.author","Hamdani, Nazha"],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Streckfuss-Bömeke, Katrin"],["dc.contributor.author","Sossalla, Samuel"],["dc.date.accessioned","2021-04-14T08:32:15Z"],["dc.date.available","2021-04-14T08:32:15Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1007/s00109-020-01989-6"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83859"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1432-1440"],["dc.relation.issn","0946-2716"],["dc.title","Long-term effects of empagliflozin on excitation-contraction-coupling in human induced pluripotent stem cell cardiomyocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]