Kües, Ursula

[["dc.bibliographiccitation.firstpage","725"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Current Trends in Biotechnology and Pharmacy"],["dc.bibliographiccitation.lastpage","731"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Kues, Ursula"],["dc.contributor.author","Rao, K.R.S.Sambasiva"],["dc.date.accessioned","2019-07-10T08:13:48Z"],["dc.date.available","2019-07-10T08:13:48Z"],["dc.date.issued","2010"],["dc.identifier.fs","568732"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7479"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61337"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Fakultät für Forstwissenschaften und Waldökologie"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","570"],["dc.title","Prof. Dr. Gopi Krishna Podila (1957-2010), an internationally renowned Indian American scientist dedicated to molecular research on trees and plant-fungal interactions - Obituary"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","788622"],["dc.bibliographiccitation.journal","Frontiers in Bioengineering and Biotechnology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Euring, Markus"],["dc.contributor.author","Ostendorf, Kolja"],["dc.contributor.author","Rühl, Martin"],["dc.contributor.author","Kües, Ursula"],["dc.date.accessioned","2022-02-16T09:02:26Z"],["dc.date.available","2022-02-16T09:02:26Z"],["dc.date.issued","2022"],["dc.description.abstract","Laccase-mediator-oxidized lignin offers replacement for conventional chemical binders to produce fiberboards. Compared to the previously reported laccase-mediator system (LMS), a lignin-laccase-mediator-system (LLMS) has an advantage in that it requires much shorter fiber-enzyme incubation time due to significantly increased redox reactions. However, the cost of regularly applying laccase on an industrial scale is currently too high. We have employed CcLcc5 from cultures of the basidiomycete Coprinopsis cinerea as a novel basi-laccase (a CAZy subfamily AA1_1 laccase) in medium-density fiberboard (MDF) production, in comparison to the commercial formulation Novozym 51003 with recombinantly produced asco-laccase MtL (a CAZy subfamily AA1_3 laccase-like multicopper oxidase from the ascomycete Myceliophthora thermophila). With the best-performing natural mediator 2,6-dimethoxyphenol (DMP), unpurified CcLcc5 was almost as good as formulated Novozym 51003 in increasing the molecular weight (MW) of the technical lignins tested, the hydrophilic high-MW Ca-lignosulfonate and the hydrophobic low-MW kraft lignin (Indulin AT). Oxygen consumption rates of the two distantly related, poorly conserved enzymes (31% sequence identity) with different mediators and lignosulfonate were also comparable, but Indulin AT significantly reduced the oxidative activity of Novozym 51003 unlike CcLcc5, regardless of the mediator used, either DMP or guaiacol. Oxygen uptake by both laccases was much faster with both technical lignins with DMP than with guaiacol. In case of lignosulfonate and DMP, 20-30 min of incubation was sufficient for full oxygen consumption, which fits in well in time with the usual binder application steps in industrial MDF production processes. LLMS-bonded MDF was thus produced on a pilot-plant scale with either crude CcLcc5 or Novozym 51003 at reduced enzyme levels of 5 kU/kg absolutely dry wood fiber with lignosulfonate and mediator DMP. Boards produced with CcLcc5 were comparably good as those made with Novozym 51003. Boards reached nearly standard specifications in internal bond strength (IB) and modulus of rupture (MOR), while thickness swelling (TS) was less good based on the hydrophilic character of lignosulfonate. LLMS-bonded MDF with Indulin AT and DMP performed better in TS but showed reduced IB and MOR values."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3389/fbioe.2021.788622"],["dc.identifier.pmid","35155404"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/99918"],["dc.language.iso","en"],["dc.relation.issn","2296-4185"],["dc.relation.orgunit","Abteilung Molekulare Holzbiotechnologie und technische Mykologie"],["dc.relation.orgunit","Zentrum für Biodiversität und Nachhaltige Landnutzung"],["dc.rights","CC BY 4.0"],["dc.title","Enzymatic Oxidation of Ca-Lignosulfonate and Kraft Lignin in Different Lignin-Laccase-Mediator-Systems and MDF Production"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1004759"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","PLoS Genetics"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hori, Chiaki"],["dc.contributor.author","Ishida, Takuya"],["dc.contributor.author","Igarashi, Kiyohiko"],["dc.contributor.author","Samejima, Masahiro"],["dc.contributor.author","Suzuki, Hitoshi"],["dc.contributor.author","Master, Emma"],["dc.contributor.author","Ferreira, Patricia"],["dc.contributor.author","Ruiz-Duenas, Francisco J."],["dc.contributor.author","Held, Benjamin W."],["dc.contributor.author","Canessa, Paulo"],["dc.contributor.author","Larrondo, Luis F."],["dc.contributor.author","Schmoll, Monika"],["dc.contributor.author","Druzhinina, Irina S."],["dc.contributor.author","Kubicek, Christian P."],["dc.contributor.author","Gaskell, Jill A."],["dc.contributor.author","Kersten, Phil"],["dc.contributor.author","St John, Franz"],["dc.contributor.author","Glasner, Jeremy"],["dc.contributor.author","Sabat, Grzegorz"],["dc.contributor.author","BonDurant, Sandra Splinter"],["dc.contributor.author","Syed, Khajamohiddin"],["dc.contributor.author","Yadav, Jagjit S."],["dc.contributor.author","Mgbeahuruike, Anthony C."],["dc.contributor.author","Kovalchuk, Andriy"],["dc.contributor.author","Asiegbu, Fred O."],["dc.contributor.author","Lackner, Gerald"],["dc.contributor.author","Hoffmeister, Dirk"],["dc.contributor.author","Rencoret, Jorge"],["dc.contributor.author","Gutierrez, Ana"],["dc.contributor.author","Sun, Hui"],["dc.contributor.author","Lindquist, Erika A."],["dc.contributor.author","Barry, Kerrie"],["dc.contributor.author","Riley, Robert W."],["dc.contributor.author","Grigoriev, Igor V."],["dc.contributor.author","Henrissat, Bernard"],["dc.contributor.author","Kuees, Ursula"],["dc.contributor.author","Berka, Randy M."],["dc.contributor.author","Martinez, Angel T."],["dc.contributor.author","Covert, Sarah F."],["dc.contributor.author","Blanchette, Robert A."],["dc.contributor.author","Cullen, Daniel"],["dc.date.accessioned","2018-11-07T09:31:52Z"],["dc.date.available","2018-11-07T09:31:52Z"],["dc.date.issued","2014"],["dc.description.abstract","Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset of these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable of colonizing freshly exposed conifer sapwood despite its high content of extractives, which retards the establishment of other fungal species. The mechanism(s) by which P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report the annotated P. gigantea genome and compare profiles of its transcriptome and secretome when cultured on fresh-cut versus solvent-extracted loblolly pine wood. The P. gigantea genome contains a conventional repertoire of hydrolase genes involved in cellulose/hemicellulose degradation, whose patterns of expression were relatively unperturbed by the absence of extractives. The expression of genes typically ascribed to lignin degradation was also largely unaffected. In contrast, genes likely involved in the transformation and detoxification of wood extractives were highly induced in its presence. Their products included an ABC transporter, lipases, cytochrome P450s, glutathione S-transferase and aldehyde dehydrogenase. Other regulated genes of unknown function and several constitutively expressed genes are also likely involved in P. gigantea's extractives metabolism. These results contribute to our fundamental understanding of pioneer colonization of conifer wood and provide insight into the diverse chemistries employed by fungi in carbon cycling processes."],["dc.identifier.doi","10.1371/journal.pgen.1004759"],["dc.identifier.fs","606903"],["dc.identifier.isi","000346649900006"],["dc.identifier.pmid","25474575"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11223"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31626"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1553-7404"],["dc.relation.issn","1553-7390"],["dc.rights.access","openAccess"],["dc.title","Analysis of the Phlebiopsis gigantea Genome, Transcriptome and Secretome Provides Insight into Its Pioneer Colonization Strategies of Wood"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e31435"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Floerl, Saskia"],["dc.contributor.author","Majcherczyk, Andrzej"],["dc.contributor.author","Possienke, Mareike"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Tappe, Hella"],["dc.contributor.author","Gatz, Christiane"],["dc.contributor.author","Feussner, I."],["dc.contributor.author","Kües, Ursula"],["dc.contributor.author","Polle, Andrea"],["dc.date.accessioned","2017-09-07T11:49:18Z"],["dc.date.available","2017-09-07T11:49:18Z"],["dc.date.issued","2012"],["dc.description.abstract","Verticillium longisporum (VL) is one of the most devastating diseases in important oil crops from the family of Brassicaceae. The fungus resides for much time of its life cycle in the extracellular fluid of the vascular system, where it cannot be controlled by conventional fungicides. To obtain insights into the biology of VL-plant interaction in the apoplast, the secretome consisting of the extracellular proteome and metabolome as well as cell wall properties were studied in the model Brassicaceae, Arabidopsis thaliana. VL infection resulted in increased production of cell wall material with an altered composition of carbohydrate polymers and increased lignification. The abundance of several hundred soluble metabolites changed in the apoplast of VL-infected plants including signalling and defence compounds such as glycosides of salicylic acid, lignans and dihydroxybenzoic acid as well as oxylipins. The extracellular proteome of healthy leaves was enriched in antifungal proteins. VL caused specific increases in six apoplast proteins (three peroxidases PRX52, PRX34, P37, serine carboxypeptidase SCPL20, α-galactosidase AGAL2 and a germin-like protein GLP3), which have functions in defence and cell wall modification. The abundance of a lectin-like, chitin-inducible protein (CILLP) was reduced. Since the transcript levels of most of the induced proteins were not elevated until late infection time points (>20 dpi), whereas those of CILLP and GLP3 were reduced at earlier time points, our results may suggest that VL enhances its virulence by rapid down-regulation and delay of induction of plant defence genes."],["dc.identifier.doi","10.1371/journal.pone.0031435"],["dc.identifier.gro","3147253"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7889"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/4884"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","chake"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Verticillium longisporum Infection Affects the Leaf Apoplastic Proteome, Metabolome, and Cell Wall Properties in Arabidopsis thaliana"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","AMB Express"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Yin, Qiang"],["dc.contributor.author","Zhou, Gang"],["dc.contributor.author","Peng, Can"],["dc.contributor.author","Zhang, Yinliang"],["dc.contributor.author","Kües, Ursula"],["dc.contributor.author","Liu, Juanjuan"],["dc.contributor.author","Xiao, Yazhong"],["dc.contributor.author","Fang, Zemin"],["dc.date.accessioned","2021-06-01T10:48:06Z"],["dc.date.available","2021-06-01T10:48:06Z"],["dc.date.issued","2019"],["dc.description.abstract","Abstract Engineering of fungal laccases with optimum catalytic activity at alkaline pH has been a long-lasting challenge. In this study, a mutant library containing 3000 clones was obtained by error-prone PCR to adapt the optimum pH of a fungal laccase Lcc9 from the basidiomycete Coprinopsis cinerea . After three rounds of functional screening, a mutant with three amino acid changes (E116K, N229D, I393T) named PIE5 was selected. PIE5 showed an optimum pH of 8.5 and 8.0 against guaiacol and 2,6-DMP when expressed in Pichia pastoris , representing the first fungal laccase that possesses an optimum pH at an alkaline condition. Site directed mutagenesis disclosed that N229D contributed the most to the optimum pH increment. A single N229D mutation caused an increase in optimum pH by 1.5 units. When used in indigo dye decolorization, PIE5 efficiently decolorized 87.1 ± 1.1% and 90.9 ± 0.3% indigo dye at the optimum conditions of pH 7.0–7.5 and 60 °C, and with either methyl 3,5-dimethoxy-4-hydroxybenzoate or 2,2′-azino-bis(3-ethylbenzothazoline-6-sulfonate) as the mediator. In comparison, the commercially available fungal laccase TvLac from Trametes villosa decolorized 84.3 ± 1.8% of indigo dye under its optimum conditions (opt. pH 5.0 and 60 °C). The properties of an alkaline-dependent activity and the high indigo dye decolorization ability (1.3-fold better than the parental Lcc9) make the new fungal laccase PIE5 an alternative for specific industrial applications."],["dc.identifier.doi","10.1186/s13568-019-0878-2"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16397"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85827"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","2191-0855"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The first fungal laccase with an alkaline pH optimum obtained by directed evolution and its application in indigo dye decolorization"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Fungal Biology and Biotechnology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Dörnte, Bastian"],["dc.contributor.author","Peng, Can"],["dc.contributor.author","Fang, Zemin"],["dc.contributor.author","Kamran, Aysha"],["dc.contributor.author","Yulvizar, Cut"],["dc.contributor.author","Kües, Ursula"],["dc.date.accessioned","2021-06-01T10:48:07Z"],["dc.date.available","2021-06-01T10:48:07Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract Background Two reference strains have been sequenced from the mushroom Coprinopsis cinerea , monokaryon Okayama 7/#130 (OK130) and the self-compatible homokaryon AmutBmut. An adenine-auxotrophy in OK130 ( ade8-1 ) and a para -aminobenzoic acid (PABA)-auxotrophy in AmutBmut ( pab1-1 ) offer selection markers for transformations. Of these two strains, homokaryon AmutBmut had been transformed before to PABA-prototrophy and with the bacterial hygromycin resistance marker hph , respectively. Results Gene ade8 encodes a bifunctional enzyme with an N-terminal glycinamide ribonucleotide synthase (GARS) and a C-terminal aminoimidazole ribonucleotide synthase (AIRS) domain required for steps 2 and 5 in the de novo biosynthesis of purines, respectively. In OK130, a missense mutation in ade8-1 rendered residue N231 for ribose recognition by the A loop of the GARS domain into D231. The new ade8 + vector p Cc Ade8 complements the auxotrophy of OK130 in transformations. Transformation rates with p Cc Ade8 in single-vector and co-transformations with ade8 + -selection were similarly high, unlike for trp1 + plasmids which exhibit suicidal feedback-effects in single-vector transformations with complementation of tryptophan synthase defects. As various other plasmids, unselected p Cc Ade8 helped in co-transformations of trp1 strains with a trp1 + -selection vector to overcome suicidal effects by transferred trp1 + . Co-transformation rates of p Cc Ade8 in OK130 under adenine selection with nuclear integration of unselected DNA were as high as 80% of clones. Co-transformation rates of expressed genes reached 26–42% for various laccase genes and up to 67% with lcc9 silencing vectors. The bacterial gene hph can also be used as another, albeit less efficient, selection marker for OK130 transformants, but with similarly high co-transformation rates. We further show that the pab1-1 defect in AmutBmut is due to a missense mutation which changed the conserved PIKGT motif for chorismate binding in the C-terminal PabB domain to PIEGT in the mutated 4-amino-4-deoxychorismate synthase. Conclusions ade8-1 and pab1-1 auxotrophic defects in C. cinerea reference strains OK130 and AmutBmut for complementation in transformation are described. p Cc Ade8 is a new transformation vector useful for selection in single and co-transformations of the sequenced monokaryon OK130 which was transformed for the first time. The bacterial gene hph can also be used as an additional selection marker in OK130, making in combination with ade8 + successive rounds of transformation possible."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1186/s40694-020-00105-0"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17606"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85835"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","2054-3085"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Selection markers for transformation of the sequenced reference monokaryon Okayama 7/#130 and homokaryon AmutBmut of Coprinopsis cinerea"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1029"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY"],["dc.bibliographiccitation.lastpage","1039"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Ruehl, Martin"],["dc.contributor.author","Majcherczyk, Andrzej"],["dc.contributor.author","Kuees, Ursula"],["dc.date.accessioned","2018-11-07T09:25:02Z"],["dc.date.available","2018-11-07T09:25:02Z"],["dc.date.issued","2013"],["dc.description.abstract","The litter-degrading dung fungus Coprinopsis cinerea has the high number of seventeen different laccase genes. In this work, ten different monokaryons were compared in their ability to produce laccases in two different complete media at different temperatures. Few strains showed laccase activity at the optimal growth temperature of 37 degrees C. Nine of the strains gave laccase activities between 0.2 and 5.9 U mL(-1) at the suboptimal temperature of 25 degrees C in mKjalke medium. Laccase activities in YMG/T medium were detected for only three strains (0.5-4.5 U mL(-1)). Zymograms of supernatants from mKjalke medium resulted in total in 10 different laccase bands but strains differed in distribution. LC-MS/MS analysis with Mascot searches of the annotated C. cinerea genome identified isoenzymes from five different genes (Lcc1, Lcc2, Lcc5, Lcc9 and Lcc10) and of Lcc1 three and of Lcc5 two distinct electrophoretical forms. Lcc1 and Lcc5 were expressed in all laccase positive strains, but not all forms were found in all of the strains. Lcc2, Lcc9 and Lcc10 occurred only in three strains as minor laccases, indicating that Lcc1 and Lcc5 are the main laccases of C. cinerea secreted in liquid mKjalke medium."],["dc.description.sponsorship","Ministry of Science and Culture of Lower Saxony [ZN 2043]"],["dc.identifier.doi","10.1007/s10482-013-9883-7"],["dc.identifier.isi","000319268600010"],["dc.identifier.pmid","23340718"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10338"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29973"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0003-6072"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Lcc1 and Lcc5 are the main laccases secreted in liquid cultures of Coprinopsis cinerea strains"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","136"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Diversity"],["dc.bibliographiccitation.lastpage","154"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Badalyan, Susanna M."],["dc.contributor.author","Szafranski, Karol"],["dc.contributor.author","Hoegger, Patrik J."],["dc.contributor.author","Navarro-González, Monica"],["dc.contributor.author","Majcherczyk, Andrzej"],["dc.contributor.author","Kües, Ursula"],["dc.date.accessioned","2019-07-09T11:54:15Z"],["dc.date.available","2019-07-09T11:54:15Z"],["dc.date.issued","2011"],["dc.description.abstract","Coprinoid mushrooms grown on wood of broad-leaf species were collected for the first time in Armenia and dikaryotic mycelial cultures were established. ITS (internal transcribed spacer) sequences identified one species as Coprinopsis strossmayeri and the other as a species closely related to Coprinellus radians. Mycelial growth and morphological features on different media are described. The pearl-white-silky colonies of C. strossmayeri are characterized by mycelial strands and by a light-yellow agar colorization. The species forms chlamydospores intercalary in its hyphae. Some hyphal ends form hyphal loops. Colonies of C. aff. radians have a characteristic yellow pigmentation and stain the agar yellowish. Hyphae are mostly clampless but at some septa, pseudoclamps are seen from which side-branches develop growing along the parental hyphae. In the mycelium of C. aff. radians, hyphal loops, hyphal swellings, cystidia and typical allocysts were observed. Both new species from Armenia show growth optima at temperatures of 25 to 30 °C and pHs of 6.0 to 7.0. Both grow in alkaline conditions up to pH 12.0 but not at pHs 3.0 and 4.0, classifying them with other coprinoid mushrooms as ―ammonia fungi‖. Both species grew on a variety of lignocellulosic substrates and both show polyphenol oxidase activities."],["dc.identifier.doi","10.3390/d3010136"],["dc.identifier.fs","576359"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8672"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60605"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1424-2818"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","New Armenian Wood-Associated Coprinoid Mushrooms: Coprinopsis strossmayeri and Coprinellus aff. radians"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","28"],["dc.bibliographiccitation.journal","AMB Express"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Lakkireddy, Kiran"],["dc.contributor.author","Kües, Ursula"],["dc.date.accessioned","2018-11-07T10:28:15Z"],["dc.date.available","2018-11-07T10:28:15Z"],["dc.date.issued","2017"],["dc.description.abstract","The basidiospores of most Agaricomycetes are ballistospores. They are propelled off from their basidia at maturity when Buller's drop develops at high humidity at the hilar spore appendix and fuses with a liquid film formed on the adaxial side of the spore. Spores are catapulted into the free air space between hymenia and fall then out of the mushroom's cap by gravity. Here we show for 66 different species that ballistospores from mushrooms can be attracted against gravity to electrostatic charged plastic surfaces. Charges on basidiospores can influence this effect. We used this feature to selectively collect basidiospores in sterile plastic Petri-dish lids from mushrooms which were positioned upside-down onto wet paper tissues for spore release into the air. Bulks of 10(4) to > 10(7) spores were obtained overnight in the plastic lids above the reversed fruiting bodies, between 10(4) and 10(6) spores already after 2-4 h incubation. In plating tests on agar medium, we rarely observed in the harvested spore solutions contaminations by other fungi (mostly none to up to in 10% of samples in different test series) and infrequently by bacteria (in between 0 and 22% of samples of test series) which could mostly be suppressed by bactericides. We thus show that it is possible to obtain clean basidiospore samples from wild mushrooms. The technique of spore collection through electrostatic attraction in plastic lids is applicable to fresh lamellate and poroid fruiting bodies from the wild, to shortlived deliquescent mushrooms, to older and dehydrating fleshy fruiting bodies, even to animal-infested mushrooms and also to dry specimens of long-lasting tough species such as Schizophyllum commune."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.description.sponsorship","German Research Foundation; Gottingen University"],["dc.identifier.doi","10.1186/s13568-017-0326-0"],["dc.identifier.isi","000392761700002"],["dc.identifier.pmid","28124290"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14319"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43382"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2191-0855"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Bulk isolation of basidiospores from wild mushrooms by electrostatic attraction with low risk of microbial contaminations"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","241"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","CURRENT TRENDS IN BIOTECHNOLOGY AND PHARMACY"],["dc.bibliographiccitation.lastpage","253"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Kües, Ursula"],["dc.contributor.author","Rühl, Martin"],["dc.date.accessioned","2019-07-10T08:13:25Z"],["dc.date.available","2019-07-10T08:13:25Z"],["dc.date.issued","2009"],["dc.description.abstract","In this study, an image analysis system for fungal pellets was developed using the commercial software system analySIS¨ and the protocol was evaluated in morphological studies of pellet formation in submerged cultures of the basidiomycete Coprinopsis cinerea. Pellets were analysed on large scale (60 to 130 pellets per image, 225 to 400 pellets per culture). Morphologies of pellets were characterised by the parameters grey value, pellet area, convexity, shape factor, sphericity and pellet diameter. Threshold values were defined for all parameters for object filtering. By application of the parameter filter, aggregated hyphal fragments present in larger amounts particularly in cultures grown at higher temperature (370C) could be clearly distinguished in image analysis from compact pellets. At a lower growth temperature (250C), there was little background of loose hyphal material, fungal pellets were regularly shaped and the pellets remained constant in shape and size over a longer cultivation period."],["dc.identifier.fs","475197"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5929"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61237"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0973-8916"],["dc.relation.orgunit","Fakultät für Forstwissenschaften und Waldökologie"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","570"],["dc.title","Automated image analysis to observe pellet morphology in liquid cultures of filamentous fungi such as the basidiomycete Coprinopsis cinerea"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]