Hubrich, Raphael

[["dc.bibliographiccitation.firstpage","308"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","318"],["dc.bibliographiccitation.volume","116"],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Park, Yongsoo"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2020-12-10T14:22:45Z"],["dc.date.available","2020-12-10T14:22:45Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.bpj.2018.11.3138"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71723"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","SNARE-Mediated Fusion of Single Chromaffin Granules with Pore-Spanning Membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","520a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Kuhlmann, Jan"],["dc.contributor.author","Schwenen, Lando L.G."],["dc.contributor.author","Milovanovic, Dragomir"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2020-12-10T14:22:42Z"],["dc.date.available","2020-12-10T14:22:42Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1016/j.bpj.2015.11.2779"],["dc.identifier.isi","000375143000026"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71699"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","Los Angeles, CA"],["dc.title","Planar Pore-Spanning Membranes: A Platform to Study Snare-Mediated Fusion Processes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","532"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Biochemical and Biophysical Research Communications"],["dc.bibliographiccitation.lastpage","537"],["dc.bibliographiccitation.volume","437"],["dc.contributor.author","Morick, Daniela"],["dc.contributor.author","Schatz, Michaela"],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Hoffmeister, Helen"],["dc.contributor.author","Krefft, Anya"],["dc.contributor.author","Witzgall, Ralph"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:47:38Z"],["dc.date.available","2017-09-07T11:47:38Z"],["dc.date.issued","2013"],["dc.description.abstract","Polycystin-2 (PC2) trafficking has been proposed to be a result of the interaction of PIGEA14 with PC2 as a function of the phosphorylation state of PC2. Here, we investigated the interaction of PIGEA14 with the C-terminal part of polycystin-2 wild type (cPC2wt) and the pseudophosphorylated mutant (cPC2S812D) to first, quantify the binding affinity between cPC2 and PIGEA14 and second, to elucidate the influence of PC2 phosphorylation on PIGEA14 binding. Solid supported membranes composed of octanethiol/1,2-dioleoyl-sn-glycero-3-phosphocholine doped with the receptor lipid DOGS-NTA-Ni were used to attach PIGEA14 to the membrane via its hexahistidine tag. By means of the quartz crystal microbalance technique, binding affinities as well as kinetic constants of the interaction were extracted in a label-free manner by applying the scaled particle theory. The results show that the dissociation constant of cPC2 to PIGEA14 is in the 10 nM regime providing strong evidence of a very specific interaction of cPC2 with PIGEA14. The interaction of cPC2wt is twofold larger than that of cPC2S812D. The moderate higher binding affinity of cPC2wt to PIGEA14 is discussed in light of PC2 trafficking to the plasma membrane. (C) 2013 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.bbrc.2013.06.105"],["dc.identifier.gro","3142309"],["dc.identifier.isi","000323584400007"],["dc.identifier.pmid","23838289"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/6853"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Dorothea Schlozer Program of the University of Gottingen"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-291X"],["dc.title","Phosphorylation of C-terminal polycystin-2 influences the interaction with PIGEA14: A QCM study based on solid supported membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Peptide Science"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Hubrich, Barbara E."],["dc.contributor.author","Wehland, Jan‐Dirk"],["dc.contributor.author","Groth, Mike C."],["dc.contributor.author","Schirmacher, Anastasiya"],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Diederichsen, Ulf"],["dc.date.accessioned","2021-06-01T09:42:26Z"],["dc.date.available","2021-06-01T09:42:26Z"],["dc.date.issued","2021"],["dc.description.abstract","Peptide‐mediated membrane fusion is frequently studied with in vitro bulk leaflet mixing assays based on Förster resonance energy transfer (FRET). In these, customized liposomes with fusogenic peptides are equipped with lipids which are labeled with fluorophores that form a FRET pair. Since FRET is dependent on distance and membrane fusion comes along with lipid mixing, the assays allow for conclusions on the membrane fusion process. The experimental outcome of these assays, however, greatly depends on the applied parameters. In the present study, the influence of the peptides, the size of liposomes, their lipid composition and the liposome stoichiometry on the fusogenicity of liposomes are evaluated. As fusogenic peptides, soluble N‐ethylmaleimide‐sensitive‐factor attachment receptor (SNARE) protein analogues featuring artificial recognition units attached to the native SNARE transmembrane domains are used. The work shows that it is important to control these parameters in order to be able to properly investigate the fusion process and to prevent undesired effects of aggregation."],["dc.description.abstract","The membrane fusion of liposomes can be mediated by soluble N‐ethylmaleimide‐sensitive‐factor attachment receptor (SNARE)‐protein derived peptide analogs. Bulk leaflet mixing assays were used to validate the fusiogenic properties of the respective peptides depending on parameters like purity, concentration, and liposome size, composition, and stoichiometry. image"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1002/psc.3327"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85251"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1099-1387"],["dc.relation.issn","1075-2617"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made."],["dc.title","Membrane fusion mediated by peptidic SNARE protein analogues: Evaluation of FRET‐based bulk leaflet mixing assays"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","12006"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.lastpage","15"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Schwenen, Lando L. G."],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Milovanovic, Dragomir"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Yang, Jian"],["dc.contributor.author","Kros, Alexander"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:43:42Z"],["dc.date.available","2017-09-07T11:43:42Z"],["dc.date.issued","2015"],["dc.description.abstract","Even though a number of different in vitro fusion assays have been developed to analyze protein mediated fusion, they still only partially capture the essential features of the in vivo situation. Here we established an in vitro fusion assay that mimics the fluidity and planar geometry of the cellular plasma membrane to be able to monitor fusion of single protein-containing vesicles. As a proof of concept, planar pore-spanning membranes harboring SNARE-proteins were generated on highly ordered functionalized 1.2 mu m-sized pore arrays in Si3N4. Full mobility of the membrane components was demonstrated by fluorescence correlation spectroscopy. Fusion was analyzed by two color confocal laser scanning fluorescence microscopy in a time resolved manner allowing to readily distinguish between vesicle docking, intermediate states such as hemifusion and full fusion. The importance of the membrane geometry on the fusion process was highlighted by comparing SNARE-mediated fusion with that of a minimal SNARE fusion mimetic."],["dc.identifier.doi","10.1038/srep12006"],["dc.identifier.fs","613746"],["dc.identifier.gro","3141864"],["dc.identifier.isi","000357847500001"],["dc.identifier.pmid","26165860"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13641"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1923"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: DFG [SFB 803, B04, B05]; Chinese Scholarship Council (CSC)"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2045-2322"],["dc.rights.access","openAccess"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Biomimetic Materials"],["dc.subject.mesh","Fluorescent Dyes"],["dc.subject.mesh","Gold"],["dc.subject.mesh","Kinetics"],["dc.subject.mesh","Membrane Fusion"],["dc.subject.mesh","Microscopy, Confocal"],["dc.subject.mesh","Models, Biological"],["dc.subject.mesh","Porosity"],["dc.subject.mesh","Rats"],["dc.subject.mesh","Recombinant Proteins"],["dc.subject.mesh","SNARE Proteins"],["dc.subject.mesh","Silicon Compounds"],["dc.subject.mesh","Time-Lapse Imaging"],["dc.subject.mesh","Unilamellar Liposomes"],["dc.title","Resolving single membrane fusion events on planar pore-spanning membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","248a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Schwenen, Lando L.G."],["dc.contributor.author","Milovanovic, Dragomir"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2020-12-10T14:22:41Z"],["dc.date.available","2020-12-10T14:22:41Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1016/j.bpj.2015.11.1364"],["dc.identifier.isi","000375141600211"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71696"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","Los Angeles, CA"],["dc.title","Synapse on a Chip: SNARE-Mediated Membrane Fusion in Planar Pore-Spanning Membranes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Schwenen, Lando L. G."],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Milovanovic, Dragomir"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Jahn, R."],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-11-07T09:55:34Z"],["dc.date.available","2018-11-07T09:55:34Z"],["dc.date.issued","2015"],["dc.format.extent","S198"],["dc.identifier.isi","000380001400599"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36777"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Dresden, GERMANY"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Synapse on a chip: SNARE-mediated fusion in planar pore-spanning membranes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]