Hubrich, Raphael

[["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Peptide Science"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Hubrich, Barbara E."],["dc.contributor.author","Wehland, Jan‐Dirk"],["dc.contributor.author","Groth, Mike C."],["dc.contributor.author","Schirmacher, Anastasiya"],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Diederichsen, Ulf"],["dc.date.accessioned","2021-06-01T09:42:26Z"],["dc.date.available","2021-06-01T09:42:26Z"],["dc.date.issued","2021"],["dc.description.abstract","Peptide‐mediated membrane fusion is frequently studied with in vitro bulk leaflet mixing assays based on Förster resonance energy transfer (FRET). In these, customized liposomes with fusogenic peptides are equipped with lipids which are labeled with fluorophores that form a FRET pair. Since FRET is dependent on distance and membrane fusion comes along with lipid mixing, the assays allow for conclusions on the membrane fusion process. The experimental outcome of these assays, however, greatly depends on the applied parameters. In the present study, the influence of the peptides, the size of liposomes, their lipid composition and the liposome stoichiometry on the fusogenicity of liposomes are evaluated. As fusogenic peptides, soluble N‐ethylmaleimide‐sensitive‐factor attachment receptor (SNARE) protein analogues featuring artificial recognition units attached to the native SNARE transmembrane domains are used. The work shows that it is important to control these parameters in order to be able to properly investigate the fusion process and to prevent undesired effects of aggregation."],["dc.description.abstract","The membrane fusion of liposomes can be mediated by soluble N‐ethylmaleimide‐sensitive‐factor attachment receptor (SNARE)‐protein derived peptide analogs. Bulk leaflet mixing assays were used to validate the fusiogenic properties of the respective peptides depending on parameters like purity, concentration, and liposome size, composition, and stoichiometry. image"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1002/psc.3327"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85251"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1099-1387"],["dc.relation.issn","1075-2617"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made."],["dc.title","Membrane fusion mediated by peptidic SNARE protein analogues: Evaluation of FRET‐based bulk leaflet mixing assays"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","12006"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.lastpage","15"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Schwenen, Lando L. G."],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Milovanovic, Dragomir"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Yang, Jian"],["dc.contributor.author","Kros, Alexander"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:43:42Z"],["dc.date.available","2017-09-07T11:43:42Z"],["dc.date.issued","2015"],["dc.description.abstract","Even though a number of different in vitro fusion assays have been developed to analyze protein mediated fusion, they still only partially capture the essential features of the in vivo situation. Here we established an in vitro fusion assay that mimics the fluidity and planar geometry of the cellular plasma membrane to be able to monitor fusion of single protein-containing vesicles. As a proof of concept, planar pore-spanning membranes harboring SNARE-proteins were generated on highly ordered functionalized 1.2 mu m-sized pore arrays in Si3N4. Full mobility of the membrane components was demonstrated by fluorescence correlation spectroscopy. Fusion was analyzed by two color confocal laser scanning fluorescence microscopy in a time resolved manner allowing to readily distinguish between vesicle docking, intermediate states such as hemifusion and full fusion. The importance of the membrane geometry on the fusion process was highlighted by comparing SNARE-mediated fusion with that of a minimal SNARE fusion mimetic."],["dc.identifier.doi","10.1038/srep12006"],["dc.identifier.fs","613746"],["dc.identifier.gro","3141864"],["dc.identifier.isi","000357847500001"],["dc.identifier.pmid","26165860"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13641"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1923"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: DFG [SFB 803, B04, B05]; Chinese Scholarship Council (CSC)"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2045-2322"],["dc.rights.access","openAccess"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Biomimetic Materials"],["dc.subject.mesh","Fluorescent Dyes"],["dc.subject.mesh","Gold"],["dc.subject.mesh","Kinetics"],["dc.subject.mesh","Membrane Fusion"],["dc.subject.mesh","Microscopy, Confocal"],["dc.subject.mesh","Models, Biological"],["dc.subject.mesh","Porosity"],["dc.subject.mesh","Rats"],["dc.subject.mesh","Recombinant Proteins"],["dc.subject.mesh","SNARE Proteins"],["dc.subject.mesh","Silicon Compounds"],["dc.subject.mesh","Time-Lapse Imaging"],["dc.subject.mesh","Unilamellar Liposomes"],["dc.title","Resolving single membrane fusion events on planar pore-spanning membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]