Janshoff, Andreas

[["dc.bibliographiccitation.firstpage","e2102026118"],["dc.bibliographiccitation.issue","27"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Schepers, Anna V."],["dc.contributor.author","Lorenz, Charlotta"],["dc.contributor.author","Nietmann, Peter"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Klumpp, Stefan"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2021-07-01T12:23:07Z"],["dc.date.available","2021-07-01T12:23:07Z"],["dc.date.issued","2021-07-06"],["dc.description.abstract","The cytoskeleton, an intricate network of protein filaments, motor proteins, and cross-linkers, largely determines the mechanical properties of cells. Among the three filamentous components, F-actin, microtubules, and intermediate filaments (IFs), the IF network is by far the most extensible and resilient to stress. We present a multiscale approach to disentangle the three main contributions to vimentin IF network mechanics—single-filament mechanics, filament length, and interactions between filaments—including their temporal evolution. Combining particle tracking, quadruple optical trapping, and computational modeling, we derive quantitative information on the strength and kinetics of filament interactions. Specifically, we find that hydrophobic contributions to network mechanics enter mostly via filament-elongation kinetics, whereas electrostatics have a direct influence on filament–filament interactions."],["dc.identifier.doi","10.1073/pnas.2102026118"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87447"],["dc.language.iso","en"],["dc.relation.issn","0027-8424"],["dc.relation.issn","1091-6490"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","cytoskeleton"],["dc.subject.gro","cellular biophysics"],["dc.title","Multiscale mechanics and temporal evolution of vimentin intermediate filament networks"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7349"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","7356"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Forsting, Johanna"],["dc.contributor.author","Kraxner, Julia"],["dc.contributor.author","Witt, Hannes"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2020-03-04T13:30:18Z"],["dc.date.available","2020-03-04T13:30:18Z"],["dc.date.issued","2019"],["dc.description.abstract","Intermediate filaments (IFs) are part of the cytoskeleton of eukaryotic cells and, therefore, are largely responsible for the cell's mechanical properties. IFs are characterized by a pronounced extensibility and remarkable resilience that enable them to support cells in extreme situations. Previous experiments showed that, under strain, α-helices in vimentin IFs might unfold to β-sheets. Upon repeated stretching, the filaments soften; however, the remaining plastic strain is negligible. Here, we observe that vimentin IFs do not recover their original stiffness on reasonable time scales, and we explain these seemingly contradicting results by introducing a third, less well-defined conformational state. Reversibility on the nanoscale can be fully rescued by introducing cross-linkers that prevent transition to the β-sheet. Our results classify IFs as a nanomaterial with intriguing mechanical properties, which is likely to play a major role for the cell's local adaption to external stimuli."],["dc.identifier.doi","10.1021/acs.nanolett.9b02972"],["dc.identifier.pmid","31498648"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63104"],["dc.language.iso","en"],["dc.relation.eissn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.relation.issn","1530-6992"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","cytoskeleton"],["dc.subject.gro","cellular biophysics"],["dc.title","Vimentin Intermediate Filaments Undergo Irreversible Conformational Changes during Cyclic Loading"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]