Betz, Timo

[["dc.contributor.author","Nguyen, Alfred"],["dc.contributor.author","Brandt, Matthias"],["dc.contributor.author","Betz, Timo"],["dc.date.accessioned","2020-11-23T10:41:30Z"],["dc.date.available","2020-11-23T10:41:30Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1101/2020.07.02.185330"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68965"],["dc.title","Microchip based microrheology via Acoustic Force Spectroscopy shows that endothelial cell mechanics follows a fractional viscoelastic model"],["dc.type","preprint"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Lab on a Chip"],["dc.contributor.author","Nguyen, Alfred"],["dc.contributor.author","Brandt, Matthias"],["dc.contributor.author","Muenker, Till M."],["dc.contributor.author","Betz, Timo"],["dc.date.accessioned","2021-04-14T08:28:33Z"],["dc.date.available","2021-04-14T08:28:33Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1039/d0lc01135e"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82645"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1473-0189"],["dc.relation.issn","1473-0197"],["dc.title","Multi-oscillation microrheology via acoustic force spectroscopy enables frequency-dependent measurements on endothelial cells at high-throughput"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0270197"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Brandt, Matthias"],["dc.contributor.author","Gerke, Volker"],["dc.contributor.author","Betz, Timo"],["dc.contributor.editor","Horowitz, Arie"],["dc.date.accessioned","2022-07-01T07:35:25Z"],["dc.date.available","2022-07-01T07:35:25Z"],["dc.date.issued","2022"],["dc.description.abstract","Endothelial cells form the inner layer of blood vessels, making them the first barrier between the blood and interstitial tissues; thus endothelial cells play a crucial role in inflammation. In the inflammatory response, one important element is the pro-inflammatory cytokine tumor necrosis factor- α (TNF- α ). While other pro-inflammatory agents like thrombin and histamine induce acute but transient changes in endothelial cells, which have been well studied biologically as well as mechanically, TNF- α is primarily known for its sustained effects on permeability and leukocyte recruitment. These functions are associated with transcriptional changes that take place on the timescale of hours and days. Here, we investigated the early mechanical action of TNF- α and show that even just 4 min after TNF- α was added onto human umbilical vein endothelial cell monolayers, there was a striking rise in mechanical substrate traction force and internal monolayer tension. These traction forces act primarily at the boundary of the monolayer, as was to be expected. This increased internal monolayer tension may, in addition to TNF- α ’s other well-studied biochemical responses, provide a mechanical signal for the cells to prepare to recruit leukocytes."],["dc.description.sponsorship","Cells in Motion Cluster of Excellence"],["dc.description.sponsorship","European Research Council"],["dc.description.sponsorship","Interdisziplinäres Zentrum für Klinische Forschung of Münster"],["dc.identifier.doi","10.1371/journal.pone.0270197"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112166"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-581"],["dc.relation.eissn","1932-6203"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Human endothelial cells display a rapid tensional stress increase in response to tumor necrosis factor-α"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2104808"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Advanced Science"],["dc.bibliographiccitation.volume","9"],["dc.contributor.affiliation","Schubert, Ann‐Sophie; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Bröker, Stephan; 3\r\nInstitute of Theoretical Physics\r\nCenter for Soft Nanoscience\r\nUniversity of Münster\r\nBusso‐Peus‐Str. 10 D‐48149 Münster Germany"],["dc.contributor.affiliation","Jurado, Alejandro; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Müller, Annika; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Brandt, Matthias; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Vos, Bart E.; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Hofemeier, Arne D.; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Abbasi, Fatemeh; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Stehling, Martin; 4\r\nMax Planck Institute for Molecular Biomedicine\r\nRöntgenstraße 20 D‐48149 Münster Germany"],["dc.contributor.affiliation","Wittkowski, Raphael; 3\r\nInstitute of Theoretical Physics\r\nCenter for Soft Nanoscience\r\nUniversity of Münster\r\nBusso‐Peus‐Str. 10 D‐48149 Münster Germany"],["dc.contributor.affiliation","Ivaska, Johanna; 5\r\nTurku Biosience Centre\r\nUniversity of Turku and Åbo Akademi University\r\nTurku FI‐20520 Finland"],["dc.contributor.author","Raghuraman, Swetha"],["dc.contributor.author","Schubert, Ann‐Sophie"],["dc.contributor.author","Bröker, Stephan"],["dc.contributor.author","Jurado, Alejandro"],["dc.contributor.author","Müller, Annika"],["dc.contributor.author","Brandt, Matthias"],["dc.contributor.author","Vos, Bart E."],["dc.contributor.author","Hofemeier, Arne D."],["dc.contributor.author","Abbasi, Fatemeh"],["dc.contributor.author","Stehling, Martin"],["dc.contributor.author","Betz, Timo"],["dc.contributor.author","Wittkowski, Raphael"],["dc.contributor.author","Ivaska, Johanna"],["dc.date.accessioned","2022-02-01T10:31:35Z"],["dc.date.available","2022-02-01T10:31:35Z"],["dc.date.issued","2022"],["dc.date.updated","2022-06-15T00:10:09Z"],["dc.description.abstract","Abstract A key behavior observed during morphogenesis, wound healing, and cancer invasion is that of collective and coordinated cellular motion. Hence, understanding the different aspects of such coordinated migration is fundamental for describing and treating cancer and other pathological defects. In general, individual cells exert forces on their environment in order to move, and collective motion is coordinated by cell–cell adhesion‐based forces. However, this notion ignores other mechanisms that encourage cellular movement, such as pressure differences. Here, using model tumors, it is found that increased pressure drove coordinated cellular motion independent of cell–cell adhesion by triggering cell swelling in a soft extracellular matrix (ECM). In the resulting phenotype, a rapid burst‐like stream of cervical cancer cells emerged from 3D aggregates embedded in soft collagen matrices (0.5 mg mL−1). This fluid‐like pushing mechanism, recorded within 8 h after embedding, shows high cell velocities and super‐diffusive motion. Because the swelling in this model system critically depends on integrin‐mediated cell–ECM adhesions and cellular contractility, the swelling is likely triggered by unsustained mechanotransduction, providing new evidence that pressure‐driven effects must be considered to more completely understand the mechanical forces involved in cell and tissue movement as well as invasion."],["dc.description.abstract","Coordinated bursts are a novel migration phenomenon where cancer cells escape from a model tumor into a soft extra‐cellular matrix made up of collagen. The interaction between the in‐homogeneous collagen and the tumor model via integrins leads to a series of dynamic effects such as cellular swelling, rise in pressure, and super‐diffusive coordinated bursts of cells into the ECM within 12 h."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.description.sponsorship","European Research Council (ERC) ‐ Consolidator"],["dc.identifier.doi","10.1002/advs.202104808"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98895"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","2198-3844"],["dc.relation.issn","2198-3844"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Pressure Drives Rapid Burst‐Like Coordinated Cellular Motion from 3D Cancer Aggregates"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1270"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Nature Physics"],["dc.bibliographiccitation.lastpage","1276"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Hurst, Sebastian"],["dc.contributor.author","Vos, Bart E."],["dc.contributor.author","Brandt, Matthias"],["dc.contributor.author","Betz, Timo"],["dc.date.accessioned","2021-12-01T09:20:53Z"],["dc.date.available","2021-12-01T09:20:53Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1038/s41567-021-01368-z"],["dc.identifier.pii","1368"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94294"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/353"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1745-2481"],["dc.relation.issn","1745-2473"],["dc.relation.workinggroup","RG Betz"],["dc.title","Intracellular softening and increased viscoelastic fluidity during division"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]