Betz, Timo

[["dc.bibliographiccitation.firstpage","1667"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","1679"],["dc.bibliographiccitation.volume","114"],["dc.contributor.author","Ahmed, Wylie W"],["dc.contributor.author","Fodor, Étienne"],["dc.contributor.author","Almonacid, Maria"],["dc.contributor.author","Bussonnier, Matthias"],["dc.contributor.author","Verlhac, Marie-Hélène"],["dc.contributor.author","Gov, Nir"],["dc.contributor.author","Visco, Paolo"],["dc.contributor.author","van Wijland, Frédéric"],["dc.contributor.author","Betz, Timo"],["dc.date.accessioned","2020-11-23T10:38:42Z"],["dc.date.available","2020-11-23T10:38:42Z"],["dc.date.issued","2018"],["dc.description.abstract","Active diffusion of intracellular components is emerging as an important process in cell biology. This process is mediated by complex assemblies of molecular motors and cytoskeletal filaments that drive force generation in the cytoplasm and facilitate enhanced motion. The kinetics of molecular motors have been precisely characterized in vitro by single molecule approaches, but their in vivo behavior remains elusive. Here, we study the active diffusion of vesicles in mouse oocytes, where this process plays a key role in nuclear positioning during development, and combine an experimental and theoretical framework to extract molecular-scale force kinetics (force, power stroke, and velocity) of the in vivo active process. Assuming a single dominant process, we find that the nonequilibrium activity induces rapid kicks of duration τ ∼ 300 μs resulting in an average force of F ∼ 0.4 pN on vesicles in in vivo oocytes, remarkably similar to the kinetics of in vitro myosin-V. Our results reveal that measuring in vivo active fluctuations allows extraction of the molecular-scale activity in agreement with single-molecule studies and demonstrates a mesoscopic framework to access force kinetics."],["dc.identifier.arxiv","1510.08299v3"],["dc.identifier.doi","10.1016/j.bpj.2018.02.009"],["dc.identifier.pmid","29642036"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68931"],["dc.language.iso","en"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Active Mechanics Reveal Molecular-Scale Force Kinetics in Living Oocytes"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","198a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Ahmed, Wylie"],["dc.contributor.author","Fodor, Etienne"],["dc.contributor.author","Almonacid, Maria"],["dc.contributor.author","Bussonnier, Matthias"],["dc.contributor.author","Verlhac, Marie-Helene"],["dc.contributor.author","Gov, Nir"],["dc.contributor.author","Visco, Paolo"],["dc.contributor.author","Wijland, Frederic van"],["dc.contributor.author","Betz, Timo"],["dc.date.accessioned","2020-11-23T10:38:22Z"],["dc.date.available","2020-11-23T10:38:22Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1016/j.bpj.2015.11.1104"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68927"],["dc.relation.issn","0006-3495"],["dc.title","Active Mechanics in Living Oocytes Reveal Molecular-Scale Force Kinetics"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","470"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Nature Cell Biology"],["dc.bibliographiccitation.lastpage","479"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Almonacid, Maria"],["dc.contributor.author","Ahmed, Wylie W."],["dc.contributor.author","Bussonnier, Matthias"],["dc.contributor.author","Mailly, Philippe"],["dc.contributor.author","Betz, Timo"],["dc.contributor.author","Voituriez, Raphaël"],["dc.contributor.author","Gov, Nir S."],["dc.contributor.author","Verlhac, Marie-Hélène"],["dc.date.accessioned","2020-11-23T10:39:30Z"],["dc.date.available","2020-11-23T10:39:30Z"],["dc.date.issued","2015-04"],["dc.description.abstract","In somatic cells, the position of the cell centroid is dictated by the centrosome. The centrosome is instrumental in nucleus positioning, the two structures being physically connected. Mouse oocytes have no centrosomes, yet harbour centrally located nuclei. We demonstrate how oocytes define their geometric centre in the absence of centrosomes. Using live imaging of oocytes, knockout for the formin 2 actin nucleator, with off-centred nuclei, together with optical trapping and modelling, we discover an unprecedented mode of nucleus positioning. We document how active diffusion of actin-coated vesicles, driven by myosin Vb, generates a pressure gradient and a propulsion force sufficient to move the oocyte nucleus. It promotes fluidization of the cytoplasm, contributing to nucleus directional movement towards the centre. Our results highlight the potential of active diffusion, a prominent source of intracellular transport, able to move large organelles such as nuclei, providing in vivo evidence of its biological function."],["dc.identifier.doi","10.1038/ncb3131"],["dc.identifier.pmid","25774831"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68940"],["dc.language.iso","en"],["dc.relation.eissn","1476-4679"],["dc.relation.issn","1465-7392"],["dc.title","Active diffusion positions the nucleus in mouse oocytes"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","353"],["dc.bibliographiccitation.lastpage","372"],["dc.contributor.author","Kopanska, Katarzyna S"],["dc.contributor.author","Bussonnier, Matthias"],["dc.contributor.author","Geraldo, Sara"],["dc.contributor.author","Simon, Anthony"],["dc.contributor.author","Vignjevic, Danijela"],["dc.contributor.author","Betz, Timo"],["dc.contributor.editor","Paluch, Ewa K."],["dc.date.accessioned","2020-11-23T10:37:30Z"],["dc.date.available","2020-11-23T10:37:30Z"],["dc.date.issued","2015"],["dc.description.abstract","Many different cell types including fibroblasts, smooth muscle cells, endothelial cells, and cancer cells exert traction forces on the fibrous components of the extracellular matrix. This can be observed as matrix contraction both macro- and microscopically in three-dimensional (3D) tissues models such as collagen type I gels. The quantification of local contraction at the micron scale, including its directionality and speed, in correlation with other parameters such as cell invasion, local protein or gene expression, can provide useful information to study wound healing, organism development, and cancer metastasis. In this article, we present a set of tools to quantify the flow dynamics of collagen contraction, induced by cells migrating out of a multicellular cancer spheroid into a three-dimensional (3D) collagen matrix. We adapted a pseudo-speckle technique that can be applied to bright-field and fluorescent microscopy time series. The image analysis presented here is based on an in-house written software developed in the Matlab (Mathworks) programming environment. The analysis program is freely available from GitHub following the link: http://dx.doi.org/10.5281/zenodo.10116. This tool provides an automatized technique to measure collagen contraction that can be utilized in different 3D cellular systems."],["dc.identifier.doi","10.1016/bs.mcb.2014.10.017"],["dc.identifier.pmid","25640438"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68918"],["dc.language.iso","en"],["dc.publisher","Elsevier"],["dc.relation.ispartof","Biophysical Methods in Cell Biology"],["dc.title","Quantification of collagen contraction in three-dimensional cell culture"],["dc.type","book_chapter"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","854"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","862"],["dc.bibliographiccitation.volume","107"],["dc.contributor.author","Bussonnier, Matthias"],["dc.contributor.author","Carvalho, Kevin"],["dc.contributor.author","Lemière, Joël"],["dc.contributor.author","Joanny, Jean-François"],["dc.contributor.author","Sykes, Cécile"],["dc.contributor.author","Betz, Timo"],["dc.date.accessioned","2020-11-23T10:38:10Z"],["dc.date.available","2020-11-23T10:38:10Z"],["dc.date.issued","2014-08-19"],["dc.description.abstract","Actin is ubiquitous globular protein that polymerizes into filaments and forms networks that participate in the force generation of eukaryotic cells. Such forces are used for cell motility, cytokinesis, and tissue remodeling. Among those actin networks, we focus on the actin cortex, a dense branched network beneath the plasma membrane that is of particular importance for the mechanical properties of the cell. Here we reproduce the cellular cortex by activating actin filament growth on a solid surface. We unveil the existence of a sparse actin network that emanates from the surface and extends over a distance that is at least 10 times larger than the cortex itself. We call this sparse actin network the \"actin cloud\" and characterize its mechanical properties with optical tweezers. We show, both experimentally and theoretically, that the actin cloud is mechanically relevant and that it should be taken into account because it can sustain forces as high as several picoNewtons (pN). In particular, it is known that in plant cells, actin networks similar to the actin cloud have a role in positioning the nucleus; in large oocytes, they play a role in driving chromosome movement. Recent evidence shows that such networks even prevent granule condensation in large cells."],["dc.identifier.doi","10.1016/j.bpj.2014.07.008"],["dc.identifier.pmid","25140420"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68925"],["dc.language.iso","en"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Mechanical detection of a long-range actin network emanating from a biomimetic cortex"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]