Betz, Timo

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hofemeier, Arne D."],["dc.contributor.author","Limon, Tamara"],["dc.contributor.author","Muenker, Till Moritz"],["dc.contributor.author","Wallmeyer, Bernhard"],["dc.contributor.author","Jurado, Alejandro"],["dc.contributor.author","Afshar, Mohammad Ebrahim"],["dc.contributor.author","Ebrahimi, Majid"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Gilbert, Penney M."],["dc.contributor.author","Betz, Timo"],["dc.date.accessioned","2021-04-14T08:29:33Z"],["dc.date.available","2021-04-14T08:29:33Z"],["dc.date.issued","2021"],["dc.description.abstract","Tension and mechanical properties of muscle tissue are tightly related to proper skeletal muscle function, which makes experimental access to the biomechanics of muscle tissue formation a key requirement to advance our understanding of muscle function and development. Recently developed elastic in vitro culture chambers allow for raising 3D muscle tissue under controlled conditions and to measure global tissue force generation. However, these chambers are inherently incompatible with high-resolution microscopy limiting their usability to global force measurements, and preventing the exploitation of modern fluorescence based investigation methods for live and dynamic measurements. Here, we present a new chamber design pairing global force measurements, quantified from post-deflection, with local tension measurements obtained from elastic hydrogel beads embedded in muscle tissue. High-resolution 3D video microscopy of engineered muscle formation, enabled by the new chamber, shows an early mechanical tissue homeostasis that remains stable in spite of continued myotube maturation."],["dc.identifier.doi","10.7554/eLife.60145"],["dc.identifier.pmid","33459593"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82931"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/118"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2050-084X"],["dc.relation.workinggroup","RG Betz"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY 4.0"],["dc.title","Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2104808"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Advanced Science"],["dc.bibliographiccitation.volume","9"],["dc.contributor.affiliation","Schubert, Ann‐Sophie; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Bröker, Stephan; 3\r\nInstitute of Theoretical Physics\r\nCenter for Soft Nanoscience\r\nUniversity of Münster\r\nBusso‐Peus‐Str. 10 D‐48149 Münster Germany"],["dc.contributor.affiliation","Jurado, Alejandro; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Müller, Annika; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Brandt, Matthias; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Vos, Bart E.; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Hofemeier, Arne D.; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Abbasi, Fatemeh; 1\r\nInstitute of Cell Biology\r\nZMBE\r\nUniversity of Münster\r\nVon‐Esmarch‐Straße 56 D‐48149 Münster Germany"],["dc.contributor.affiliation","Stehling, Martin; 4\r\nMax Planck Institute for Molecular Biomedicine\r\nRöntgenstraße 20 D‐48149 Münster Germany"],["dc.contributor.affiliation","Wittkowski, Raphael; 3\r\nInstitute of Theoretical Physics\r\nCenter for Soft Nanoscience\r\nUniversity of Münster\r\nBusso‐Peus‐Str. 10 D‐48149 Münster Germany"],["dc.contributor.affiliation","Ivaska, Johanna; 5\r\nTurku Biosience Centre\r\nUniversity of Turku and Åbo Akademi University\r\nTurku FI‐20520 Finland"],["dc.contributor.author","Raghuraman, Swetha"],["dc.contributor.author","Schubert, Ann‐Sophie"],["dc.contributor.author","Bröker, Stephan"],["dc.contributor.author","Jurado, Alejandro"],["dc.contributor.author","Müller, Annika"],["dc.contributor.author","Brandt, Matthias"],["dc.contributor.author","Vos, Bart E."],["dc.contributor.author","Hofemeier, Arne D."],["dc.contributor.author","Abbasi, Fatemeh"],["dc.contributor.author","Stehling, Martin"],["dc.contributor.author","Betz, Timo"],["dc.contributor.author","Wittkowski, Raphael"],["dc.contributor.author","Ivaska, Johanna"],["dc.date.accessioned","2022-02-01T10:31:35Z"],["dc.date.available","2022-02-01T10:31:35Z"],["dc.date.issued","2022"],["dc.date.updated","2022-06-15T00:10:09Z"],["dc.description.abstract","Abstract A key behavior observed during morphogenesis, wound healing, and cancer invasion is that of collective and coordinated cellular motion. Hence, understanding the different aspects of such coordinated migration is fundamental for describing and treating cancer and other pathological defects. In general, individual cells exert forces on their environment in order to move, and collective motion is coordinated by cell–cell adhesion‐based forces. However, this notion ignores other mechanisms that encourage cellular movement, such as pressure differences. Here, using model tumors, it is found that increased pressure drove coordinated cellular motion independent of cell–cell adhesion by triggering cell swelling in a soft extracellular matrix (ECM). In the resulting phenotype, a rapid burst‐like stream of cervical cancer cells emerged from 3D aggregates embedded in soft collagen matrices (0.5 mg mL−1). This fluid‐like pushing mechanism, recorded within 8 h after embedding, shows high cell velocities and super‐diffusive motion. Because the swelling in this model system critically depends on integrin‐mediated cell–ECM adhesions and cellular contractility, the swelling is likely triggered by unsustained mechanotransduction, providing new evidence that pressure‐driven effects must be considered to more completely understand the mechanical forces involved in cell and tissue movement as well as invasion."],["dc.description.abstract","Coordinated bursts are a novel migration phenomenon where cancer cells escape from a model tumor into a soft extra‐cellular matrix made up of collagen. The interaction between the in‐homogeneous collagen and the tumor model via integrins leads to a series of dynamic effects such as cellular swelling, rise in pressure, and super‐diffusive coordinated bursts of cells into the ECM within 12 h."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.description.sponsorship","European Research Council (ERC) ‐ Consolidator"],["dc.identifier.doi","10.1002/advs.202104808"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98895"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","2198-3844"],["dc.relation.issn","2198-3844"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Pressure Drives Rapid Burst‐Like Coordinated Cellular Motion from 3D Cancer Aggregates"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]