Brügmann, Tobias

[["dc.bibliographiccitation.firstpage","1765"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Wagdi, Ahmed"],["dc.contributor.author","Malan, Daniela"],["dc.contributor.author","Sathyanarayanan, Udhayabhaskar"],["dc.contributor.author","Beauchamp, Janosch S."],["dc.contributor.author","Vogt, Markus"],["dc.contributor.author","Zipf, David"],["dc.contributor.author","Beiert, Thomas"],["dc.contributor.author","Mansuroglu, Berivan"],["dc.contributor.author","Dusend, Vanessa"],["dc.contributor.author","Meininghaus, Mark"],["dc.contributor.author","Schneider, Linn"],["dc.contributor.author","Kalthof, Bernd"],["dc.contributor.author","Wiegert, J. Simon"],["dc.contributor.author","König, Gabriele M."],["dc.contributor.author","Kostenis, Evi"],["dc.contributor.author","Patejdl, Robert"],["dc.contributor.author","Sasse, Philipp"],["dc.contributor.author","Bruegmann, Tobias"],["dc.date.accessioned","2022-04-06T07:25:22Z"],["dc.date.available","2022-04-06T07:25:22Z"],["dc.date.issued","2022-04-01"],["dc.description.abstract","Gq proteins are universally important for signal transduction in mammalian cells. The underlying kinetics and transformation from extracellular stimuli into intracellular signaling, however could not be investigated in detail so far. Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution. Properties and G protein specificity of hOPN5 are characterized by UV light induced IP3 generation, Ca2+ transients and inhibition of GIRK channel activity in HEK cells. In adult hearts from a transgenic animal model, light increases the spontaneous beating rate. In addition, we demonstrate light induced contractions in the small intestine, which are not detectable after pharmacological Gq protein block. All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening. Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.1038/s41467-022-29265-w"],["dc.identifier.pmid","35365606"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106429"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/467"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/426"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A14: Gq Protein Aktivierung durch Dehnung der Atrien bei Herzinsuffizienz (HF)"],["dc.relation.issn","2041-1723"],["dc.relation.workinggroup","RG Brügmann (Vegetative Optogenetik)"],["dc.rights","CC BY 4.0"],["dc.title","Selective optogenetic control of Gq signaling using human Neuropsin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","675"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Sasse, Philipp"],["dc.contributor.author","Funken, Maximilian"],["dc.contributor.author","Beiert, Thomas"],["dc.contributor.author","Brügmann, Tobias"],["dc.date.accessioned","2019-07-09T11:51:50Z"],["dc.date.available","2019-07-09T11:51:50Z"],["dc.date.issued","2019"],["dc.description.abstract","Optogenetic methods enable selective de- and hyperpolarization of cardiomyocytes expressing light-sensitive proteins within the myocardium. By using light, this technology provides very high spatial and temporal precision, which is in clear contrast to electrical stimulation. In addition, cardiomyocyte-specific expression would allow pain-free stimulation. In light of these intrinsic technical advantages, optogenetic methods provide an intriguing opportunity to understand and improve current strategies to terminate cardiac arrhythmia as well as for possible pain-free arrhythmia termination in patients in the future. In this review, we give a concise introduction to optogenetic stimulation of cardiomyocytes and the whole heart and summarize the recent progress on optogenetic defibrillation and cardioversion to terminate cardiac arrhythmia. Toward this aim, we specifically focus on the different mechanisms of optogenetic arrhythmia termination and how these might influence the prerequisites for success. Furthermore, we critically discuss the clinical perspectives and potential patient populations, which might benefit from optogenetic defibrillation devices."],["dc.identifier.doi","10.3389/fphys.2019.00675"],["dc.identifier.pmid","31244670"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16204"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60021"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Optogenetic Termination of Cardiac Arrhythmia: Mechanistic Enlightenment and Therapeutic Application?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]