Brügmann, Tobias

[["dc.bibliographiccitation.firstpage","1765"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Wagdi, Ahmed"],["dc.contributor.author","Malan, Daniela"],["dc.contributor.author","Sathyanarayanan, Udhayabhaskar"],["dc.contributor.author","Beauchamp, Janosch S."],["dc.contributor.author","Vogt, Markus"],["dc.contributor.author","Zipf, David"],["dc.contributor.author","Beiert, Thomas"],["dc.contributor.author","Mansuroglu, Berivan"],["dc.contributor.author","Dusend, Vanessa"],["dc.contributor.author","Meininghaus, Mark"],["dc.contributor.author","Schneider, Linn"],["dc.contributor.author","Kalthof, Bernd"],["dc.contributor.author","Wiegert, J. Simon"],["dc.contributor.author","König, Gabriele M."],["dc.contributor.author","Kostenis, Evi"],["dc.contributor.author","Patejdl, Robert"],["dc.contributor.author","Sasse, Philipp"],["dc.contributor.author","Bruegmann, Tobias"],["dc.date.accessioned","2022-04-06T07:25:22Z"],["dc.date.available","2022-04-06T07:25:22Z"],["dc.date.issued","2022-04-01"],["dc.description.abstract","Gq proteins are universally important for signal transduction in mammalian cells. The underlying kinetics and transformation from extracellular stimuli into intracellular signaling, however could not be investigated in detail so far. Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution. Properties and G protein specificity of hOPN5 are characterized by UV light induced IP3 generation, Ca2+ transients and inhibition of GIRK channel activity in HEK cells. In adult hearts from a transgenic animal model, light increases the spontaneous beating rate. In addition, we demonstrate light induced contractions in the small intestine, which are not detectable after pharmacological Gq protein block. All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening. Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.1038/s41467-022-29265-w"],["dc.identifier.pmid","35365606"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106429"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/467"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/426"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A14: Gq Protein Aktivierung durch Dehnung der Atrien bei Herzinsuffizienz (HF)"],["dc.relation.issn","2041-1723"],["dc.relation.workinggroup","RG Brügmann (Vegetative Optogenetik)"],["dc.rights","CC BY 4.0"],["dc.title","Selective optogenetic control of Gq signaling using human Neuropsin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5562"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","22"],["dc.contributor.affiliation","Rehnelt, Susanne; \t\t \r\n\t\t Institute of Physiology I, Medical Faculty, University of Bonn, 53127 Bonn, Germany, s4surehn@uni-bonn.de"],["dc.contributor.affiliation","Malan, Daniela; \t\t \r\n\t\t Institute of Physiology I, Medical Faculty, University of Bonn, 53127 Bonn, Germany, dmalan@uni-bonn.de"],["dc.contributor.affiliation","Juhasz, Krisztina; \t\t \r\n\t\t Nanion Technologies GmbH, 80636 Munich, Germany, krisztina.juhasz@nanion.de"],["dc.contributor.affiliation","Wolters, Benjamin; \t\t \r\n\t\t Part of the Ncardia Group, Axiogenesis AG, 50829 Cologne, Germany, benjamin.wolters@ncardia.com"],["dc.contributor.affiliation","Doerr, Leo; \t\t \r\n\t\t Nanion Technologies GmbH, 80636 Munich, Germany, leo.doerr@nanion.de"],["dc.contributor.affiliation","Beckler, Matthias; \t\t \r\n\t\t Nanion Technologies GmbH, 80636 Munich, Germany, matthias.beckler@nanion.de"],["dc.contributor.affiliation","Kettenhofen, Ralf; \t\t \r\n\t\t Part of the Ncardia Group, Axiogenesis AG, 50829 Cologne, Germany, ralf.kettenhofen@ncardia.com"],["dc.contributor.affiliation","Bohlen, Heribert; \t\t \r\n\t\t Part of the Ncardia Group, Axiogenesis AG, 50829 Cologne, Germany, heribert.bohlen@ncardia.com"],["dc.contributor.affiliation","Bruegmann, Tobias; \t\t \r\n\t\t Institute of Physiology I, Medical Faculty, University of Bonn, 53127 Bonn, Germany, tobias.bruegmann@med.uni-goettingen.de\t\t \r\n\t\t Research Training Group 1873, University of Bonn, 53127 Bonn, Germany, tobias.bruegmann@med.uni-goettingen.de"],["dc.contributor.affiliation","Sasse, Philipp; \t\t \r\n\t\t Institute of Physiology I, Medical Faculty, University of Bonn, 53127 Bonn, Germany, philipp.sasse@uni-bonn.de"],["dc.contributor.author","Rehnelt, Susanne"],["dc.contributor.author","Malan, Daniela"],["dc.contributor.author","Juhasz, Krisztina"],["dc.contributor.author","Wolters, Benjamin"],["dc.contributor.author","Doerr, Leo"],["dc.contributor.author","Beckler, Matthias"],["dc.contributor.author","Kettenhofen, Ralf"],["dc.contributor.author","Bohlen, Heribert"],["dc.contributor.author","Brügmann, Tobias"],["dc.contributor.author","Sasse, Philipp"],["dc.date.accessioned","2021-07-05T15:00:46Z"],["dc.date.available","2021-07-05T15:00:46Z"],["dc.date.issued","2021"],["dc.date.updated","2022-11-11T13:15:08Z"],["dc.description.abstract","The authors wish to make the following corrections to this paper [...]"],["dc.description.abstract","The authors wish to make the following corrections to this paper [...]"],["dc.identifier.doi","10.3390/ijms22115562"],["dc.identifier.pii","ijms22115562"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87899"],["dc.language.iso","en"],["dc.notes.intern","DOI Import DOI-Import GROB-441"],["dc.publisher","MDPI"],["dc.relation.eissn","1422-0067"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Addendum: Rehnelt et al. Frequency-Dependent Multi-Well Cardiotoxicity Screening Enabled by Optogenetic Stimulation. Int. J. Mol. Sci. 2017, 18, 2634"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","498"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Funken, Maximilian"],["dc.contributor.author","Malan, Daniela"],["dc.contributor.author","Sasse, Philipp"],["dc.contributor.author","Brügmann, Tobias"],["dc.date.accessioned","2019-07-09T11:51:15Z"],["dc.date.available","2019-07-09T11:51:15Z"],["dc.date.issued","2019"],["dc.description.abstract","Cardiac defibrillation to terminate lethal ventricular arrhythmia (VA) is currently performed by applying high energy electrical shocks. In cardiac tissue, electrical shocks induce simultaneously de- and hyperpolarized areas and only depolarized areas are considered to be responsible for VA termination. Because electrical shocks do not allow proper control over spatial extent and level of membrane potential changes, the effects of hyperpolarization have not been explored in the intact heart. In contrast, optogenetic methods allow cell type-selective induction of de- and hyperpolarization with unprecedented temporal and spatial control. To investigate effects of cardiomyocyte hyperpolarization on VA termination, we generated a mouse line with cardiomyocyte-specific expression of the light-driven proton pump ArchT. Isolated cardiomyocytes showed light-induced outward currents and hyperpolarization. Free-running VA were evoked by electrical stimulation of explanted hearts perfused with low K+ and the KATP channel opener Pinacidil. Optogenetic hyperpolarization was induced by epicardial illumination, which terminated VA with an average efficacy of ∼55%. This value was significantly higher compared to control hearts without illumination or ArchT expression (p = 0.0007). Intracellular recordings with sharp electrodes within the intact heart revealed hyperpolarization and faster action potential upstroke upon illumination, which should fasten conduction. However, conduction speed was lower during illumination suggesting enhanced electrical sink by hyperpolarization underlying VA termination. Thus, selective hyperpolarization in cardiomyocytes is able to terminate VA with a completely new mechanism of increased electrical sink. These novel insights could improve our mechanistic understanding and treatment strategies of VA termination."],["dc.identifier.doi","10.3389/fphys.2019.00498"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16086"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59908"],["dc.language.iso","en"],["dc.notes.intern","DeepGreen Import"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-042X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.subject.ddc","610"],["dc.title","Optogenetic Hyperpolarization of Cardiomyocytes Terminates Ventricular Arrhythmia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Müllenbroich, M. Caroline"],["dc.contributor.author","Kelly, Allen"],["dc.contributor.author","Acker, Corey"],["dc.contributor.author","Bub, Gil"],["dc.contributor.author","Brügmann, Tobias"],["dc.contributor.author","Di Bona, Anna"],["dc.contributor.author","Entcheva, Emilia"],["dc.contributor.author","Ferrantini, Cecilia"],["dc.contributor.author","Kohl, Peter"],["dc.contributor.author","Lehnart, Stephan E."],["dc.contributor.author","Smith, Godfrey L."],["dc.contributor.author","Mongillo, Marco"],["dc.contributor.author","Parmeggiani, Camilla"],["dc.contributor.author","Richter, Claudia"],["dc.contributor.author","Sasse, Philipp"],["dc.contributor.author","Zaglia, Tania"],["dc.contributor.author","Sacconi, Leonardo"],["dc.date.accessioned","2022-01-11T14:05:21Z"],["dc.date.available","2022-01-11T14:05:21Z"],["dc.date.issued","2021"],["dc.description.abstract","Optical techniques for recording and manipulating cellular electrophysiology have advanced rapidly in just a few decades. These developments allow for the analysis of cardiac cellular dynamics at multiple scales while largely overcoming the drawbacks associated with the use of electrodes. The recent advent of optogenetics opens up new possibilities for regional and tissue-level electrophysiological control and hold promise for future novel clinical applications. This article, which emerged from the international NOTICE workshop in 2018 1 , reviews the state-of-the-art optical techniques used for cardiac electrophysiological research and the underlying biophysics. The design and performance of optical reporters and optogenetic actuators are reviewed along with limitations of current probes. The physics of light interaction with cardiac tissue is detailed and associated challenges with the use of optical sensors and actuators are presented. Case studies include the use of fluorescence recovery after photobleaching and super-resolution microscopy to explore the micro-structure of cardiac cells and a review of two photon and light sheet technologies applied to cardiac tissue. The emergence of cardiac optogenetics is reviewed and the current work exploring the potential clinical use of optogenetics is also described. Approaches which combine optogenetic manipulation and optical voltage measurement are discussed, in terms of platforms that allow real-time manipulation of whole heart electrophysiology in open and closed-loop systems to study optimal ways to terminate spiral arrhythmias. The design and operation of optics-based approaches that allow high-throughput cardiac electrophysiological assays is presented. Finally, emerging techniques of photo-acoustic imaging and stress sensors are described along with strategies for future development and establishment of these techniques in mainstream electrophysiological research."],["dc.identifier.doi","10.3389/fphys.2021.769586"],["dc.identifier.pmid","34867476"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97650"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/436"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/170"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P03: Erhaltung und funktionelle Kopplung von ER-Kontakten mit der Plasmamembran"],["dc.relation.eissn","1664-042X"],["dc.relation.workinggroup","RG Lehnart"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","overview_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","675"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Sasse, Philipp"],["dc.contributor.author","Funken, Maximilian"],["dc.contributor.author","Beiert, Thomas"],["dc.contributor.author","Brügmann, Tobias"],["dc.date.accessioned","2019-07-09T11:51:50Z"],["dc.date.available","2019-07-09T11:51:50Z"],["dc.date.issued","2019"],["dc.description.abstract","Optogenetic methods enable selective de- and hyperpolarization of cardiomyocytes expressing light-sensitive proteins within the myocardium. By using light, this technology provides very high spatial and temporal precision, which is in clear contrast to electrical stimulation. In addition, cardiomyocyte-specific expression would allow pain-free stimulation. In light of these intrinsic technical advantages, optogenetic methods provide an intriguing opportunity to understand and improve current strategies to terminate cardiac arrhythmia as well as for possible pain-free arrhythmia termination in patients in the future. In this review, we give a concise introduction to optogenetic stimulation of cardiomyocytes and the whole heart and summarize the recent progress on optogenetic defibrillation and cardioversion to terminate cardiac arrhythmia. Toward this aim, we specifically focus on the different mechanisms of optogenetic arrhythmia termination and how these might influence the prerequisites for success. Furthermore, we critically discuss the clinical perspectives and potential patient populations, which might benefit from optogenetic defibrillation devices."],["dc.identifier.doi","10.3389/fphys.2019.00675"],["dc.identifier.pmid","31244670"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16204"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60021"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Optogenetic Termination of Cardiac Arrhythmia: Mechanistic Enlightenment and Therapeutic Application?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]