Seikowski, Jan

[["dc.bibliographiccitation.artnumber","5859"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Lin, Chao-Chen"],["dc.contributor.author","Seikowski, Jan"],["dc.contributor.author","Perez-Lara, Angel"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Höbartner, Claudia"],["dc.contributor.author","Walla, Peter Jomo"],["dc.date.accessioned","2018-11-07T09:31:47Z"],["dc.date.available","2018-11-07T09:31:47Z"],["dc.date.issued","2014"],["dc.description.abstract","Fast synchronous neurotransmitter release is triggered by calcium that activates synaptotagmin-1 (syt-1), resulting in fusion of synaptic vesicles with the presynaptic membrane. Syt-1 possesses two Ca2+-binding C2 domains that tether membranes via interactions with anionic phospholipids. It is capable of crosslinking membranes and has recently been speculated to trigger fusion by decreasing the gap between them. As quantitative information on membrane gaps is key to understanding general cellular mechanisms, including the role of syt-1, we developed a fluorescence-lifetime based inter-membrane distance ruler using membrane-anchored DNAs of various lengths as calibration standards. Wild-type and mutant data provide evidence that full-length syt-1 indeed regulates membrane gaps: without Ca2+, syt-1 maintains membranes at distances of similar to 7-8 nm. Activation with 100 mu M Ca2+ decreases the distance to similar to 5 nm by binding the C2 domains to opposing membranes, respectively. These values reveal that activated syt-1 adjusts membrane distances to the level that promotes SNARE complex assembly."],["dc.identifier.doi","10.1038/ncomms6859"],["dc.identifier.isi","000347683100001"],["dc.identifier.pmid","25500905"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31610"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","2041-1723"],["dc.title","Control of membrane gaps by synaptotagmin-Ca2+ measured with a novel membrane distance ruler"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5505"],["dc.bibliographiccitation.issue","14"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","5509"],["dc.bibliographiccitation.volume","59"],["dc.contributor.author","Savicheva, Elizaveta A."],["dc.contributor.author","Mitronova, Guyzel Yu."],["dc.contributor.author","Thomas, Laura"],["dc.contributor.author","Böhm, Marvin J."],["dc.contributor.author","Seikowski, Jan"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2021-04-14T08:27:44Z"],["dc.date.available","2021-04-14T08:27:44Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1002/anie.201908063"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82385"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1521-3773"],["dc.relation.issn","1433-7851"],["dc.title","Negatively Charged Yellow‐Emitting 1‐Aminopyrene Dyes for Reductive Amination and Fluorescence Detection of Glycans"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5329"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Analytical Chemistry"],["dc.bibliographiccitation.lastpage","5336"],["dc.bibliographiccitation.volume","92"],["dc.contributor.author","Fomin, Maksim A."],["dc.contributor.author","Seikowski, Jan"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:45:38Z"],["dc.date.available","2022-03-01T11:45:38Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1021/acs.analchem.9b05863"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103400"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1520-6882"],["dc.relation.issn","0003-2700"],["dc.title","Negatively Charged Red-Emitting Acridine Dyes for Facile Reductive Amination, Separation, and Fluorescent Detection of Glycans"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1912"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","1916"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Samanta, Biswajit"],["dc.contributor.author","Seikowski, Jan"],["dc.contributor.author","Hoebartner, Claudia"],["dc.date.accessioned","2018-11-07T10:19:10Z"],["dc.date.available","2018-11-07T10:19:10Z"],["dc.date.issued","2016"],["dc.description.abstract","5-Formylcytosine (5fC) and 5-formyluracil (5fU) are natural nucleobase modifications that are generated by oxidative modification of 5-methylcytosine and thymine (or 5-methyluracil). Herein, we describe chemoselective labeling of 5-formylpyrimidine nucleotides in DNA and RNA by fluorogenic aldol-type condensation reactions with 2,3,3-trimethylindole derivatives. Mild and specific reaction conditions were developed for 5fU and 5fC to produce hemicyanine-like chromophores with distinct photophysical properties. Residue-specific detection was established by fluorescence readout as well as primer-extension assays. The reactions were optimized on DNA oligonucleotides and were equally suitable for the modification of 5fU- and 5fC-modified RNA. This direct labeling approach of 5-formylpyrimidines is expected to help in elucidating the occurrence, enzymatic transformations, and functional roles of these epigenetic/epitranscriptomic nucleobase modifications in DNA and RNA."],["dc.identifier.doi","10.1002/anie.201508893"],["dc.identifier.isi","000369854000061"],["dc.identifier.pmid","26679556"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41610"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-v C H Verlag Gmbh"],["dc.relation.issn","1521-3773"],["dc.relation.issn","1433-7851"],["dc.title","Fluorogenic Labeling of 5-Formylpyrimidine Nucleotides in DNA and RNA"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3720"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","3726"],["dc.bibliographiccitation.volume","60"],["dc.contributor.author","Savicheva, Elizaveta A."],["dc.contributor.author","Seikowski, Jan"],["dc.contributor.author","Kast, Jeannette I."],["dc.contributor.author","Grünig, Christoph R."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:45:02Z"],["dc.date.available","2022-03-01T11:45:02Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1002/anie.202013187"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103193"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1521-3773"],["dc.relation.issn","1433-7851"],["dc.rights.uri","http://creativecommons.org/licenses/by-nc-nd/4.0/"],["dc.title","Fluorescence Assisted Capillary Electrophoresis of Glycans Enabled by the Negatively Charged Auxochromes in 1‐Aminopyrenes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","15128"],["dc.bibliographiccitation.issue","50"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","15133"],["dc.bibliographiccitation.volume","54"],["dc.contributor.author","Kosutic, Marija"],["dc.contributor.author","Neuner, Sandra"],["dc.contributor.author","Ren, Aiming"],["dc.contributor.author","Fluer, Sara"],["dc.contributor.author","Wunderlich, Christaph"],["dc.contributor.author","Mairhofer, Elisabeth"],["dc.contributor.author","Vusurovic, Nikola"],["dc.contributor.author","Seikowski, Jan"],["dc.contributor.author","Breuket, Kathrin"],["dc.contributor.author","Hoebartner, Claudia"],["dc.contributor.author","Patel, Dinshaw J."],["dc.contributor.author","Kreutz, Christoph"],["dc.contributor.author","Micura, Ronald"],["dc.date.accessioned","2018-11-07T09:47:37Z"],["dc.date.available","2018-11-07T09:47:37Z"],["dc.date.issued","2015"],["dc.description.abstract","Nucleolytic ribozymes catalyze site-specific cleavage of their phosphodiester backbones. A minimal version of the twister ribozyme is reported that lacks the phylogenetically conserved stem P1 while retaining wild-type activity. Atomic mutagenesis revealed that nitrogen atoms N1 and N3 of the adenine-6 at the cleavage site are indispensable for cleavage. By NMR spectroscopy, a pK(a) value of 5.1 was determined for a (13)C2-labeled adenine at this position in the twister ribozyme, which is significantly shifted compared to the pKa of the same adenine in the substrate alone. This finding pinpoints at a potential role for adenine-6 in the catalytic mechanism besides the previously identified invariant guanine-48 and a Mg2+ ion, both of which are directly coordinated to the non-bridging oxygen atoms of the scissile phosphate; for the latter, additional evidence stems from the observation that Mn2+ or Cd2+ accelerated cleavage of phosphorothioate substrates. The relevance of this metal ion binding site is further emphasized by a new 2.6 angstrom X-ray structure of a 2'-OCH3- U5 modified twister ribozyme."],["dc.identifier.doi","10.1002/anie.201506601"],["dc.identifier.isi","000368057400023"],["dc.identifier.pmid","26473980"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35151"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-v C H Verlag Gmbh"],["dc.relation.issn","1521-3773"],["dc.relation.issn","1433-7851"],["dc.title","A Mini-Twister Variant and Impact of Residues/Cations on the Phosphodiester Cleavage of this Ribozyme Class"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7267"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","The Journal of Organic Chemistry"],["dc.bibliographiccitation.lastpage","7275"],["dc.bibliographiccitation.volume","85"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Stoldt, Stefan"],["dc.contributor.author","Rüttger, Franziska"],["dc.contributor.author","John, Michael"],["dc.contributor.author","Seikowski, Jan"],["dc.contributor.author","Schimpfhauser, Jens"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2021-04-14T08:25:52Z"],["dc.date.available","2021-04-14T08:25:52Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1021/acs.joc.0c00653"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81752"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1520-6904"],["dc.relation.issn","0022-3263"],["dc.title","Synthesis of Fluorescent Jasplakinolide Analogues for Live-Cell STED Microscopy of Actin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]