Richter, Claudia

[["dc.bibliographiccitation.artnumber","750535"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Diaz-Maue, Laura"],["dc.contributor.author","Steinebach, Janna"],["dc.contributor.author","Richter, Claudia"],["dc.date.accessioned","2022-10-06T13:26:46Z"],["dc.date.available","2022-10-06T13:26:46Z"],["dc.date.issued","2022"],["dc.description.abstract","Much has been reported about optogenetic based cardiac arrhythmia treatment and the corresponding characterization of photostimulation parameters, but still, our capacity to interact with the underlying spatiotemporal excitation patterns relies mainly on electrical and/or pharmacological approaches. However, these well-established treatments have always been an object of somehow heated discussions. Though being acutely life-saving, they often come with potential side-effects leading to a decreased functionality of the complex cardiac system. Recent optogenetic studies showed the feasibility of the usage of photostimulation as a defibrillation method with comparatively high success rates. Although, these studies mainly concentrated on the description as well as on the comparison of single photodefibrillation approaches, such as locally focused light application and global illumination, less effort was spent on the description of excitation patterns during actual photostimulation. In this study, the authors implemented a multi-site photodefibrillation technique in combination with Multi-Lead electrocardiograms (ECGs). The technical connection of real-time heart rhythm measurements and the arrhythmia counteracting light control provides a further step toward automated arrhythmia classification, which can lead to adaptive photodefibrillation methods. In order to show the power effectiveness of the new approach, transgenic murine hearts expressing channelrhodopsin-2\n ex vivo\n were investigated using circumferential micro-LED and ECG arrays. Thus, combining the best of two methods by giving the possibility to illuminate either locally or globally with differing pulse parameters. The optical technique presented here addresses a number of challenges of technical cardiac optogenetics and is discussed in the context of arrhythmic development during photostimulation."],["dc.identifier.doi","10.3389/fphys.2021.750535"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115163"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1664-042X"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Patterned Illumination Techniques in Optogenetics: An Insight Into Decelerating Murine Hearts"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1651"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Quiñonez Uribe, Raúl A."],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Diaz-Maue, Laura"],["dc.contributor.author","Richter, Claudia"],["dc.date.accessioned","2019-07-09T11:50:14Z"],["dc.date.available","2019-07-09T11:50:14Z"],["dc.date.issued","2018"],["dc.description.abstract","Complex spatiotemporal non-linearity as observed during cardiac arrhythmia strongly correlates with vortex-like excitation wavelengths and tissue characteristics. Therefore, the control of arrhythmic patterns requires fundamental understanding of dependencies between onset and perpetuation of arrhythmia and substrate instabilities. Available treatments, such as drug application or high-energy electrical shocks, are discussed for potential side effects resulting in prognosis worsening due to the lack of specificity and spatiotemporal precision. In contrast, cardiac optogenetics relies on light sensitive ion channels stimulated to trigger excitation of cardiomyocytes solely making use of the inner cell mechanisms. This enables low-energy, non-damaging optical control of cardiac excitation with high resolution. Recently, the capability of optogenetic cardioversion was shown in Channelrhodopsin-2 (ChR2) transgenic mice. But these studies used mainly structured and local illumination for cardiac stimulation. In addition, since optogenetic and electrical stimulus work on different principles to control the electrical activity of cardiac tissue, a better understanding of the phenomena behind optogenetic cardioversion is still needed. The present study aims to investigate global illumination with regard to parameter characterization and its potential for cardioversion. Our results show that by tuning the light intensity without exceeding 1.10 mW mm-2, a single pulse in the range of 10-1,000 ms is sufficient to reliably reset the heart into sinus rhythm. The combination of our panoramic low-intensity photostimulation with optical mapping techniques visualized wave collision resulting in annihilation as well as propagation perturbations as mechanisms leading to optogenetic cardioversion, which seem to base on other processes than electrical defibrillation. This study contributes to the understanding of the roles played by epicardial illumination, pulse duration and light intensity in optogenetic cardioversion, which are the main variables influencing cardiac optogenetic control, highlighting the advantages and insights of global stimulation. Therefore, the presented results can be modules in the design of novel illumination technologies with specific energy requirements on the way toward tissue-protective defibrillation techniques."],["dc.identifier.doi","10.3389/fphys.2018.01651"],["dc.identifier.pmid","30542292"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15889"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59730"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/302"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/241526/EU//EUTRIGTREAT"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation","SFB 1002 | C03: Erholung nach Herzinsuffizienz: Analyse der transmuralen mechano-elektrischen Funktionsstörung"],["dc.relation.eissn","1664-042X"],["dc.relation.issn","1664-042X"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.subject.ddc","610"],["dc.title","Energy-Reduced Arrhythmia Termination Using Global Photostimulation in Optogenetic Murine Hearts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","174"],["dc.bibliographiccitation.journal","Journal of Visualized Experiments"],["dc.contributor.author","Diaz-Maue, Laura"],["dc.contributor.author","Steinebach, Janna"],["dc.contributor.author","Schwaerzle, Michael"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Ruther, Patrick"],["dc.contributor.author","Richter, Claudia"],["dc.date.accessioned","2021-12-01T09:23:13Z"],["dc.date.available","2021-12-01T09:23:13Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.3791/62335"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94593"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","1940-087X"],["dc.title","Advanced Cardiac Rhythm Management by Applying Optogenetic Multi-Site Photostimulation in Murine Hearts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","104820G"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.seriesnr","1048219"],["dc.contributor.author","Diaz-Maue, Laura"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Richter, Claudia"],["dc.contributor.editor","Mohanty, Samarendra K."],["dc.contributor.editor","Thakor, Nitish V."],["dc.contributor.editor","Jansen, E. Duco"],["dc.date.accessioned","2019-02-27T16:47:31Z"],["dc.date.available","2019-02-27T16:47:31Z"],["dc.date.issued","2018"],["dc.description.abstract","Detailed understanding of mechanisms and instabilities underlying the onset, perpetuation, and control of cardiac arrhythmias is required for the development, further optimization, and translation of clinically applicable defibrillation methods. Recently, the potential use of optogenetic tools using structured illumination to control cardiac arrhythmia has been successfully demonstrated and photostimulation turned out to be a promising experimental tool to investigate the dynamics and mechanisms of multi-site pacing strategies for low-energy defibrillation. In order to study the relation between trigger and control mechanisms of arrhythmic cardiac conditions without external affecting factors like eventually damaging fiber poking, it is important to establish a non-invasive photostimulation method. Hence, we applied a custom-configured digital light processing micromirror array operated by a high-speed FPGA, which guarantees a high frequency control of stimulation patterns. The integration into a highly sophisticated optical experiment setup allows us to record photostimulation effects and to proof the light pulse as origin of cardiac excitation. Experiments with transgenic murine hearts demonstrate the successful induction and termination of cardiac dysrhythmia using light crafting tools. However, the complex spatiotemporal dynamics underlying arrhythmia critically depends on the ratio of the characteristic wavelength of arrhythmia and substrate size. Based on the experimental evidence regarding the feasibility of optical defibrillation in small mammals, the transfer in clinically relevant large animal models would be the next milestone to therapeutic translation. Thus, the presented experimental results of optogenetically modified murine hearts function as originator for ongoing studies involving principle design studies for therapeutic applicable optical defibrillation."],["dc.identifier.doi","10.1117/12.2288976"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/57656"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/207"],["dc.language.iso","en"],["dc.notes.status","fcwi"],["dc.publisher","SPIE"],["dc.publisher.place","Bellingham, Washington"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C03: Erholung nach Herzinsuffizienz: Analyse der transmuralen mechano-elektrischen Funktionsstörung"],["dc.relation.conference","SPIE BiOS"],["dc.relation.crisseries","Proceedings of SPIE"],["dc.relation.eventend","2018-01-28"],["dc.relation.eventlocation","San Francisco"],["dc.relation.eventstart","2018-01-27"],["dc.relation.isbn","978-1-5106-1449-9"],["dc.relation.isbn","978-1-5106-1450-5"],["dc.relation.ispartof","Optogenetics and Optical Manipulation 2018"],["dc.relation.ispartofseries","Proceedings of SPIE; 1048219"],["dc.relation.issn","1605-7422"],["dc.relation.issn","1996-756X"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.title","Towards optogenetic control of spatiotemporal cardiac dynamics"],["dc.type","conference_paper"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]