Drummer, Charis

[["dc.bibliographiccitation.firstpage","505"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Vogt, Edgar-John"],["dc.contributor.author","Heistermann, Michael"],["dc.contributor.author","Roshani, Berit"],["dc.contributor.author","Becker, Tamara"],["dc.contributor.author","Mätz-Rensing, Kerstin"],["dc.contributor.author","Kues, Wilfried A."],["dc.contributor.author","Kügler, Sebastian"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2021-06-01T09:42:32Z"],["dc.date.available","2021-06-01T09:42:32Z"],["dc.date.issued","2021"],["dc.description.abstract","Genetic modification of non-human primates (NHP) paves the way for realistic disease models. The common marmoset is a NHP species increasingly used in biomedical research. Despite the invention of RNA-guided nucleases, one strategy for protein overexpression in NHP is still lentiviral transduction. We generated three male and one female enhanced green fluorescent protein (EGFP)-transgenic founder marmosets via lentiviral transduction of natural preimplantation embryos. All founders accomplished germline transmission of the transgene by natural mating, yielding 20 transgenic offspring together (in total, 45 pups; 44% transgenic). This demonstrates that the transgenic gametes are capable of natural fertilization even when in competition with wildtype gametes. Importantly, 90% of the transgenic offspring showed transgene silencing, which is in sharp contrast to rodents, where the identical transgene facilitated robust EGFP expression. Furthermore, we consistently discovered somatic, but so far, no germ cell chimerism in mixed wildtype/transgenic litters. Somatic cell chimerism resulted in false-positive genotyping of the respective wildtype littermates. For the discrimination of transgenic from transgene-chimeric animals by polymerase chain reaction on skin samples, a chimeric cell depletion protocol was established. In summary, it is possible to establish a cohort of genetically modified marmosets by natural mating, but specific requirements including careful promoter selection are essential."],["dc.identifier.doi","10.3390/cells10030505"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85277"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2073-4409"],["dc.title","Generation and Breeding of EGFP-Transgenic Marmoset Monkeys: Cell Chimerism and Implications for Disease Modeling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2414"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Animals"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Münzker, Julia"],["dc.contributor.author","Heistermann, Michael"],["dc.contributor.author","Becker, Tamara"],["dc.contributor.author","Mißbach, Sophie"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2022-10-04T10:21:49Z"],["dc.date.available","2022-10-04T10:21:49Z"],["dc.date.issued","2022"],["dc.description.abstract","Non-human primates (NHPs) serve as embryo donors for embryo collection in order to mimic genetic diseases in humans by genetic modification. Reproductive health of the embryo donors is crucial, and chronic distress needs to be avoided. Embryo retrieval rates (ERR), anti-Müllerian hormone (AMH) concentrations, cortisol levels, and body weight fluctuations were assessed as markers for fertility and distress. With regard to successful embryo retrievals (total n = 667), the animals were either used for extended periods (long-term group; LTG) or only for short periods (short-term group; STG). Retrospective evaluation expectedly showed that animals in the LTG had a higher ERR than animals in the STG (p < 0.0001). Importantly, ERR in the LTG remained stable throughout the experimental period, and high embryo rates were already encountered during the first year of experimental use (p = 0.0002). High ERR were associated with high AMH and low cortisol levels, and minimal body weight fluctuations following anesthesia, indicating a superior ability of the LTG animals to handle distress. We conclude that the long-term experimental use of marmosets does not impair their fertility or health status per se, supporting the view that animal reuse can be in accordance with the 3R-principle, implying reduction, replacement, and refinement in animal experimentation."],["dc.identifier.doi","10.3390/ani12182414"],["dc.identifier.pii","ani12182414"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114507"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","2076-2615"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Performance of Marmoset Monkeys as Embryo Donors Is Reflected by Different Stress-Related Parameters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","23"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Primate Biology"],["dc.bibliographiccitation.lastpage","28"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Daskalaki, Maria"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Heistermann, Michael"],["dc.date.accessioned","2022-09-01T09:51:20Z"],["dc.date.available","2022-09-01T09:51:20Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract. Alfaxan® (alfaxalone) is a steroid general anesthetic widely used in veterinary medicine for induction and maintenance of anesthesia in several species. While the use of alfaxalone in veterinary practice has several benefits compared to the use of other anesthetic agents, the fact that it is derived from progesterone may confound the measurement of the latter in the blood of animals under alfaxalone treatment. In the present case study, we report the measurement of serum progesterone in an individual common marmoset (Callithrix jacchus) during five ovarian cycles in which luteolysis was\ninduced by PGF2α. Blood samples were usually taken from the awake\nanimal with the exception of the fifth cycle in which the sample was\ncollected under alfaxalone anesthesia in connection with a tooth extraction. In contrast to the previous four cycles in which luteolysis resulted in the expected marked decrease in progesterone concentrations, the – apparent – progesterone level in the cycle under alfaxalone treatment remained unexpectedly high. Cross-reactivity of the non-specific antibody used in the progesterone assay with alfaxalone most likely explains this finding."],["dc.identifier.doi","10.5194/pb-9-23-2022"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113936"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","2363-4715"],["dc.title","The use of alfaxalone for short-term anesthesia can confound serum progesterone measurements in the common marmoset: a case report"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2480298"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Stem Cells International"],["dc.bibliographiccitation.lastpage","17"],["dc.bibliographiccitation.volume","2016"],["dc.contributor.author","Fereydouni, Bentolhoda"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Heistermann, Michael"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Lewerich, Lucia"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2018-11-07T10:21:30Z"],["dc.date.accessioned","2019-02-26T14:51:35Z"],["dc.date.available","2018-11-07T10:21:30Z"],["dc.date.available","2019-02-26T14:51:35Z"],["dc.date.issued","2016"],["dc.description.abstract","We use the common marmoset monkey (Callithrix jacchus) as a preclinical nonhuman primate model to study reproductive and stem cell biology. The neonatal marmoset monkey ovary contains numerous primitive premeiotic germ cells (oogonia) expressing pluripotent stem cell markers including OCT4A (POU5F1). This is a peculiarity compared to neonatal human and rodent ovaries. Here, we aimed at culturing marmoset oogonia from neonatal ovaries. We established a culture system being stable for more than 20 passages and 5 months. Importantly, comparative transcriptome analysis of the cultured cells with neonatal ovary, embryonic stem cells, and fibroblasts revealed a lack of germ cell and pluripotency genes indicating the complete loss of oogonia upon initiation of the culture. From passage 4 onwards, however, the cultured cells produced large spherical, free-floating cells resembling oocyte-like cells (OLCs). OLCs strongly expressed several germ cell genes and may derive from the ovarian surface epithelium. In summary, our novel primate ovarian cell culture initially lacked detectable germ cells but then produced OLCs over a long period of time. This culture system may allow a deeper analysis of early phases of female primate germ cell development and—after significant refinement—possibly also the production of monkey oocytes."],["dc.identifier.doi","10.1155/2016/2480298"],["dc.identifier.isi","000367975300001"],["dc.identifier.pmid","26664406"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12864"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42105"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/57622"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/126"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","Najko"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C05: Bedeutung von zellulären Immunreaktionen für das kardiale Remodeling und die Therapie der Herzinsuffizienz durch Stammzelltransplantation"],["dc.relation.issn","1687-9678"],["dc.relation.issn","1687-966X"],["dc.relation.workinggroup","RG Dressel"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]