Drummer, Charis

[["dc.bibliographiccitation.firstpage","477"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","MHR Basic science of reproductive medicine"],["dc.bibliographiccitation.lastpage","488"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Aeckerle, Nelia"],["dc.contributor.author","Eildermann, K."],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Ehmcke, J."],["dc.contributor.author","Schweyer, Stefan"],["dc.contributor.author","Lerchl, A."],["dc.contributor.author","Bergmann, M."],["dc.contributor.author","Kliesch, S."],["dc.contributor.author","Gromoll, Joerg"],["dc.contributor.author","Schlatt, S."],["dc.contributor.author","Behr, R."],["dc.date.accessioned","2018-11-07T09:05:18Z"],["dc.date.available","2018-11-07T09:05:18Z"],["dc.date.issued","2012"],["dc.description.abstract","Mammalian spermatogenesis is maintained by spermatogonial stem cells (SSCs). However, since evidentiary assays and unequivocal markers are still missing in non-human primates (NHPs) and man, the identity of primate SSCs is unknown. In contrast, in mice, germ cell transplantation studies have functionally demonstrated the presence of SSCs. LIN28 is an RNA-binding pluripotent stem cell factor, which is also strongly expressed in undifferentiated mouse spermatogonia. By contrast, two recent reports indicated that LIN28 is completely absent from adult human testes. Here, we analyzed LIN28 expression in marmoset monkey (Callithrix jacchus) and human testes during development and adulthood and compared it with that in mice. In the marmoset, LIN28 was strongly expressed in migratory primordial germ cells and gonocytes. Strikingly, we found a rare LIN28-positive subpopulation of spermatogonia also in adult marmoset testis. This was corroborated by western blotting and quantitative RTPCR. Importantly, in contrast to previous publications, we found LIN28-positive spermatogonia also in normal adult human and additional adult NHP testes. Some seasonal breeders exhibit a degenerated (involuted) germinal epithelium consisting only of Sertoli cells and SSCs during their non-breeding season. The latter re-initiate spermatogenesis prior to the next breeding-season. Fully involuted testes from a seasonal hamster and NHP (Lemur catta) exhibited numerous LIN28-positive spermatogonia, indicating an SSC identity of the labeled cells. We conclude that LIN28 is differentially expressed in mouse and NHP spermatogonia and might be a marker for a rare SSC population in NHPs and man. Further characterization of the LIN28-positive population is required."],["dc.identifier.doi","10.1093/molehr/gas025"],["dc.identifier.isi","000309463100002"],["dc.identifier.pmid","22689537"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25285"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1460-2407"],["dc.relation.issn","1360-9947"],["dc.title","The pluripotency factor LIN28 in monkey and human testes: a marker for spermatogonial stem cells?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","MHR Basic science of reproductive medicine"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Aeckerle, Nelia"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Debowski, Katharina"],["dc.contributor.author","Viebahn, Christoph"],["dc.contributor.author","Behr, R."],["dc.date.accessioned","2018-11-07T09:56:32Z"],["dc.date.available","2018-11-07T09:56:32Z"],["dc.date.issued","2015"],["dc.format.extent","552"],["dc.identifier.doi","10.1093/molehr/gav016"],["dc.identifier.isi","000356261000007"],["dc.identifier.pmid","26026067"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36973"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1460-2407"],["dc.relation.issn","1360-9947"],["dc.title","Primordial germ cell development in the marmoset monkey as revealed by pluripotency factor expression: suggestion of a novel model of embryonic germ cell translocation (vol 21, pg 66, 2015)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","66"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","MHR Basic science of reproductive medicine"],["dc.bibliographiccitation.lastpage","80"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Aeckerle, Nelia"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Debowski, Katharina"],["dc.contributor.author","Viebahn, Christoph"],["dc.contributor.author","Behr, R."],["dc.date.accessioned","2018-11-07T10:03:32Z"],["dc.date.available","2018-11-07T10:03:32Z"],["dc.date.issued","2015"],["dc.description.abstract","Primordial germ cells (PGCs) are the embryonic progenitors of sperm and egg cells. Mammalian PGCs are thought to actively migrate from the yolk sac endoderm over long distances across the embryo to reach the somatic genital ridges. The general principles of mammalian PGC development were discovered in mice. In contrast, little is known about PGC development in primates due to extremely limited access to primate embryos. Here, we analyzed 12 well preserved marmoset monkey (Callithrix jacchus) embryos covering the phase from PGC emergence in the endoderm to the formation of the sexually differentiated gonad (embryonic day (E) 50 to E95). We show using immunohistochemistry that the pluripotency factors OCT4A and NANOG specifically mark PGCs throughout the period studied. In contrast, SALL4 and LIN28 were first expressed ubiquitously and only later down-regulated in somatic tissues. We further show, for the first time, that PGCs are located in the endoderm in E50 embryos in close spatial proximity to the prospective genital ridge, making a long-range migration of PGCs dispensable. At E65, PGCs are already present in the primitive gonad, while significantly later embryonic stages still exhibit PGCs at their original endodermal site, revealing a wide spatio-temporal window of PGC distribution. Our findings challenge the 'dogma' of active long-range PGC migration from the endoderm to the gonads. We therefore favor an alternative model based primarily on passive translocation of PGCs from the mesenchyme that surrounds the gut to the prospective gonad through the intercalar expansion of mesenchymal tissue which contains the PGCs. In summary, we (i) show differential pluripotency factor expression during primate embryo development and (ii) provide a schematic model for embryonic PGC translocation."],["dc.identifier.doi","10.1093/molehr/gau088"],["dc.identifier.isi","000350052200008"],["dc.identifier.pmid","25237007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38488"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1460-2407"],["dc.relation.issn","1360-9947"],["dc.title","Primordial germ cell development in the marmoset monkey as revealed by pluripotency factor expression: suggestion of a novel model of embryonic germ cell translocation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]