Sidenstein, Sven C.

[["dc.bibliographiccitation.artnumber","26725"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","D'Este, Elisa"],["dc.contributor.author","Böhm, Marvin J."],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:44:54Z"],["dc.date.available","2017-09-07T11:44:54Z"],["dc.date.issued","2016"],["dc.description.abstract","Superresolution fluorescence microscopy of multiple fluorophores still requires development. Here we present simultaneous three-colour stimulated emission depletion (STED) nanoscopy relying on a single STED beam at 620 nm. Toggling the STED beam between two or more power levels (\"multilevelSTED\") optimizes resolution and contrast in all colour channels, which are intrinsically co-aligned and well separated. Three-colour recording is demonstrated by imaging the nanoscale cytoskeletal organization in cultured hippocampal neurons. The down to similar to 35 nm resolution identified periodic actin/betaII spectrin lattices along dendrites and spines; however, at presynaptic and postsynaptic sites, these patterns were found to be absent. Both our multicolour scheme and the 620 nm STED line should be attractive for routine STED microscopy applications."],["dc.identifier.doi","10.1038/srep26725"],["dc.identifier.gro","3141682"],["dc.identifier.isi","000376500200002"],["dc.identifier.pmid","27220554"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13375"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8850"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2045-2322"],["dc.rights.access","openAccess"],["dc.title","Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]