Kliesch, Torben-Tobias

[["dc.bibliographiccitation.firstpage","206"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cells Tissues Organs"],["dc.bibliographiccitation.lastpage","220"],["dc.bibliographiccitation.volume","196"],["dc.contributor.author","Eildermann, K."],["dc.contributor.author","Aeckerle, Nelia"],["dc.contributor.author","Debowski, Katharina"],["dc.contributor.author","Godmann, M."],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Heistermann, Michael"],["dc.contributor.author","Schweyer, Stefan"],["dc.contributor.author","Bergmann, M."],["dc.contributor.author","Kliesch, S."],["dc.contributor.author","Gromoll, Joerg"],["dc.contributor.author","Ehmcke, J."],["dc.contributor.author","Schlatt, S."],["dc.contributor.author","Behr, R."],["dc.date.accessioned","2018-11-07T09:14:59Z"],["dc.date.available","2018-11-07T09:14:59Z"],["dc.date.issued","2012"],["dc.description.abstract","SALL4 (sal-like protein 4) is a pluripotency transcription factor, which is highly expressed in embryonic stem (ES) cells and which is essential for mouse preimplantation development. In adult mouse organs, Sall4 mRNA is highly expressed in the testis and ovary, while there is only little or no expression in other organs. There is also a high expression of SALL4 in human testicular germ cell tumors. However, there is as yet no detailed analysis of SALL4 expression during mammalian testicular development. We analyzed SALL4 expression in ES cells, preimplantation embryos, and the developing and adult testis of a nonhuman primate (NHP) species, the common marmoset monkey (Callithrix jacchus). Immunofluorescence revealed SALL4 in the nuclei of marmoset ES cells and preimplantation embryos. Marmoset SALL4 isoform analysis in ES cells and newborn and adult testis by RTPCR and Western blotting showed two different isoforms, SALL4-A and SALL4-B. Immunohistochemistry localized this transcription factor to the nuclei of primordial germ cells and most gonocytes in the prenatal and early postnatal marmoset testis. In the pubertal and adult testis SALL4 was present in undifferentiated spermatogonia. In the developing and adult human and mouse testis SALL4 expression mimicked the pattern in the marmoset. Adult testes from additional NHP species, the treeshrew, the cat and the dog also exhibited SALL4 in undifferentiated spermatogonia, indicating a conserved expression in the mammalian testis. Taking into account the importance of SALL4 for mouse development, we conclude that SALL4 may play an important role during mammalian germ cell development and is involved in the regulation of spermatogonial proliferation in the adult testis. Copyright (C) 2012 S. Karger AG, Basel"],["dc.identifier.doi","10.1159/000335031"],["dc.identifier.isi","000308266400002"],["dc.identifier.pmid","22572102"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9081"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27561"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.relation.issn","1422-6405"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Developmental Expression of the Pluripotency Factor Sal-Like Protein 4 in the Monkey, Human and Mouse Testis: Restriction to Premeiotic Germ Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","477"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","MHR Basic science of reproductive medicine"],["dc.bibliographiccitation.lastpage","488"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Aeckerle, Nelia"],["dc.contributor.author","Eildermann, K."],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Ehmcke, J."],["dc.contributor.author","Schweyer, Stefan"],["dc.contributor.author","Lerchl, A."],["dc.contributor.author","Bergmann, M."],["dc.contributor.author","Kliesch, S."],["dc.contributor.author","Gromoll, Joerg"],["dc.contributor.author","Schlatt, S."],["dc.contributor.author","Behr, R."],["dc.date.accessioned","2018-11-07T09:05:18Z"],["dc.date.available","2018-11-07T09:05:18Z"],["dc.date.issued","2012"],["dc.description.abstract","Mammalian spermatogenesis is maintained by spermatogonial stem cells (SSCs). However, since evidentiary assays and unequivocal markers are still missing in non-human primates (NHPs) and man, the identity of primate SSCs is unknown. In contrast, in mice, germ cell transplantation studies have functionally demonstrated the presence of SSCs. LIN28 is an RNA-binding pluripotent stem cell factor, which is also strongly expressed in undifferentiated mouse spermatogonia. By contrast, two recent reports indicated that LIN28 is completely absent from adult human testes. Here, we analyzed LIN28 expression in marmoset monkey (Callithrix jacchus) and human testes during development and adulthood and compared it with that in mice. In the marmoset, LIN28 was strongly expressed in migratory primordial germ cells and gonocytes. Strikingly, we found a rare LIN28-positive subpopulation of spermatogonia also in adult marmoset testis. This was corroborated by western blotting and quantitative RTPCR. Importantly, in contrast to previous publications, we found LIN28-positive spermatogonia also in normal adult human and additional adult NHP testes. Some seasonal breeders exhibit a degenerated (involuted) germinal epithelium consisting only of Sertoli cells and SSCs during their non-breeding season. The latter re-initiate spermatogenesis prior to the next breeding-season. Fully involuted testes from a seasonal hamster and NHP (Lemur catta) exhibited numerous LIN28-positive spermatogonia, indicating an SSC identity of the labeled cells. We conclude that LIN28 is differentially expressed in mouse and NHP spermatogonia and might be a marker for a rare SSC population in NHPs and man. Further characterization of the LIN28-positive population is required."],["dc.identifier.doi","10.1093/molehr/gas025"],["dc.identifier.isi","000309463100002"],["dc.identifier.pmid","22689537"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25285"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1460-2407"],["dc.relation.issn","1360-9947"],["dc.title","The pluripotency factor LIN28 in monkey and human testes: a marker for spermatogonial stem cells?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]