Kliesch, Torben-Tobias

[["dc.bibliographiccitation.firstpage","10463"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","Langmuir"],["dc.bibliographiccitation.lastpage","10474"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Schaefer, Edith"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:21:12Z"],["dc.date.available","2018-11-07T09:21:12Z"],["dc.date.issued","2013"],["dc.description.abstract","The mechanical response of giant liposomes to compression between two parallel plates is investigated in the context of an artificial actin cortex adjacent to the inner leaflet the bilayer. We found that nonlinear membrane theory neglecting the impact of bending sufficiently describes the mechanical response of liposomes consisting of fluid lipids to compression -whereasthe formation of an actin cortex or the use of gel-phase, lipids generally leads to substantial stiffening of the shell. Giant vesicles are gently adsorbed on glassy surfaces and are compressed with tipless cantilevers using an atomic force microscope. Force-compression curves display a nonlinear response that allows us to determine the membrane tension sigma(0) and the area compressibility modulus K-A by computing the contour of the vesicle as a function of the compression depth. The values for K-A of fluid membranes correspond well to what is known from micropipet-suction experiments and computed from monitoring membrane undulations. The presence of a thick actin shell adjacent to the inner leaflet of the liposome membrane stiffens the system considerably, as mirrored in a significantly higher apparent area compressibility modulus."],["dc.description.sponsorship","DFG [SFB 803]"],["dc.identifier.doi","10.1021/la401969t"],["dc.identifier.isi","000323472000020"],["dc.identifier.pmid","23869855"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29057"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0743-7463"],["dc.title","Mechanical Properties of Giant Liposomes Compressed between Two Parallel Plates: Impact of Artificial Actin Shells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4487"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Soft Matter"],["dc.bibliographiccitation.lastpage","4495"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Schaefer, Edith"],["dc.contributor.author","Vache, Marian"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:03:13Z"],["dc.date.available","2018-11-07T10:03:13Z"],["dc.date.issued","2015"],["dc.description.abstract","Indentation of giant liposomes with a conical indenter is described by means of a tension-based membrane model. We found that nonlinear membrane theory neglecting the impact of bending sufficiently describes the mechanical response of liposomes to indentation as measured by atomic force microscopy. Giant vesicles are gently adsorbed on glassy surfaces via avidin-biotin linkages and indented centrally using an atomic force microscope equipped with conventional sharp tips mounted on top of an inverted microscope. Force indentation curves display a nonlinear response that allows to extract pre-stress of the bilayer T-0 and the area compressibility modulus K-A by computing the contour of the vesicle at a given force. The values for K-A of fluid membranes correspond well to what is known from micropipet suction experiments and inferred from membrane undulation monitoring. Assembly of actin shells inside the liposome considerably stiffens the vesicles resulting in significantly larger area compressibility modules. The analysis can be easily extended to different indenter geometries with rotational symmetry."],["dc.description.sponsorship","[SFB 803 (B08)]"],["dc.identifier.doi","10.1039/c5sm00191a"],["dc.identifier.isi","000355555100018"],["dc.identifier.pmid","25946988"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12612"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38409"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","1744-6848"],["dc.relation.issn","1744-683X"],["dc.rights.access","openAccess"],["dc.title","Mechanical response of adherent giant liposomes to indentation with a conical AFM-tip"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Kliesch, T.-T."],["dc.contributor.author","Schaefer, Edith"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:22:33Z"],["dc.date.available","2018-11-07T09:22:33Z"],["dc.date.issued","2013"],["dc.format.extent","S124"],["dc.identifier.isi","000330215300342"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29367"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Lisbon, PORTUGAL"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Investigation of membrane fusion as a function of lateral membrane tension"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]