Kliesch, Torben-Tobias

[["dc.bibliographiccitation.firstpage","388"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Strahlentherapie und Onkologie"],["dc.bibliographiccitation.lastpage","405"],["dc.bibliographiccitation.volume","176"],["dc.contributor.author","Souchon, R."],["dc.contributor.author","Krege, S."],["dc.contributor.author","Schmoll, Hans-Joachim"],["dc.contributor.author","Albers, P."],["dc.contributor.author","Beyer, J."],["dc.contributor.author","Bokemeyer, Carsten"],["dc.contributor.author","Classen, Joseph"],["dc.contributor.author","Dieckmann, K. P."],["dc.contributor.author","Hartmann, M."],["dc.contributor.author","Heidenreich, A."],["dc.contributor.author","Holtl, W."],["dc.contributor.author","Kliesch, S."],["dc.contributor.author","Kohrmann, K. U."],["dc.contributor.author","Kuczyk, M."],["dc.contributor.author","Schmidberger, Heinz"],["dc.contributor.author","Weinknecht, S."],["dc.contributor.author","Winter, E."],["dc.contributor.author","Wittekind, Christian"],["dc.contributor.author","Bamberg, M."],["dc.date.accessioned","2018-11-07T10:32:47Z"],["dc.date.available","2018-11-07T10:32:47Z"],["dc.date.issued","2000"],["dc.description.abstract","Background: An \"Interdisciplinary Consensus Statement on the Diagnosis and Therapy of Testicular Tumors\" was prepared in 1996 by the \"Interdisciplinary Testicular Tumor Working Group\" (IAH) with input from representatives from diagnostic and therapeutic disciplines of various working groups of the German Cancer Society (Strahlenther Onkol 1997;173:397-406), In 1998 the IAH met again together with the \"Testicular Tumor Working Party\" of the Urooncology Working Group (AUO) and formed the \"German Testicular Cancer Study Group\" (GTCSG). Defined and accepted interdisciplinary standards from the initial meeting were revised based on current scientific developments and clinical results. This cooperating effort increased the quality of the initial recommendations and helped to put the recommendations for diagnosing and treating testicular tumor on a broader scientific basis. Methods: According to the principles of \"evidence-based medicine\" (EBM), the Consensus from 1996 was modified, based on the current level of evidence from the published literature. The methodological process and evaluation criteria used were that of the \"Cochrane Collaboration\". Results: An \"Interdisciplinary Update Consensus Statement\" summarizes and defines the diagnostic and therapeutic standards according to the current scientific practices in testicular cancer. For 21 separate areas scientifically based decision criteria are suggested. For treatment areas where more than one option exist without a consensus being reached for a preferred strategy, such as in seminoma in clinical Stage I or in non-seminoma Stages CS I or CS IIA/B, all acceptable alternative strategies with their respective advantages and disadvantages are presented. This \"Interdisciplinary Update Consensus\" was presented at the 24th National Congress of the German Cancer Society on March 21st and subsequently evaluated and approved by the various German scientific medical societies."],["dc.identifier.doi","10.1007/PL00002347"],["dc.identifier.isi","000089215900002"],["dc.identifier.pmid","11050912"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/44437"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Vogel"],["dc.relation.issn","0179-7158"],["dc.title","Interdisciplinary consensus on diagnosis and treatment of testicular germ cell tumors. Results of an update conference based on evidence-based medicine (EBM)"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10463"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","Langmuir"],["dc.bibliographiccitation.lastpage","10474"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Schaefer, Edith"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:21:12Z"],["dc.date.available","2018-11-07T09:21:12Z"],["dc.date.issued","2013"],["dc.description.abstract","The mechanical response of giant liposomes to compression between two parallel plates is investigated in the context of an artificial actin cortex adjacent to the inner leaflet the bilayer. We found that nonlinear membrane theory neglecting the impact of bending sufficiently describes the mechanical response of liposomes consisting of fluid lipids to compression -whereasthe formation of an actin cortex or the use of gel-phase, lipids generally leads to substantial stiffening of the shell. Giant vesicles are gently adsorbed on glassy surfaces and are compressed with tipless cantilevers using an atomic force microscope. Force-compression curves display a nonlinear response that allows us to determine the membrane tension sigma(0) and the area compressibility modulus K-A by computing the contour of the vesicle as a function of the compression depth. The values for K-A of fluid membranes correspond well to what is known from micropipet-suction experiments and computed from monitoring membrane undulations. The presence of a thick actin shell adjacent to the inner leaflet of the liposome membrane stiffens the system considerably, as mirrored in a significantly higher apparent area compressibility modulus."],["dc.description.sponsorship","DFG [SFB 803]"],["dc.identifier.doi","10.1021/la401969t"],["dc.identifier.isi","000323472000020"],["dc.identifier.pmid","23869855"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29057"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0743-7463"],["dc.title","Mechanical Properties of Giant Liposomes Compressed between Two Parallel Plates: Impact of Artificial Actin Shells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3540"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","3544"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Lambertz, Christina"],["dc.contributor.author","Martos, Ariadna"],["dc.contributor.author","Henkel, Andreas"],["dc.contributor.author","Neiser, Andreas"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Schwille, Petra"],["dc.contributor.author","Soennichsen, Carsten"],["dc.date.accessioned","2018-11-07T10:13:31Z"],["dc.date.available","2018-11-07T10:13:31Z"],["dc.date.issued","2016"],["dc.description.abstract","We use individual gold nanorods as pointlike detectors for the intrinsic dynamics of an oscillating biological system. We chose the pattern forming MinDE protein system from Escherichia coli (E. coli), a prominent example for self organized chemical oscillations of membrane-associated proteins that are involved in the bacterial cell division process. Similar to surface plasmon resonance (SPR), the gold nanorods report changes in their protein surface coverage without the need for fluorescence labeling, a technique we refer to as NanoSPR Comparing the dynamics for fluorescence labeled and unlabeled proteins, we find a reduction of the oscillation period by about 20%. The absence of photobleaching allows us to investigate Min proteins attaching and detaching from lipid coated gold nanorods with an unprecedented bandwidth of 100 ms time resolution and 1 h observation time. The long observation reveals small changes of the oscillation period over time. Averaging many cycles yields the precise wave profile that exhibits the four phases suggested in previous reports. Unexpected from previous fluorescence-based studies, we found an immobile static protein layer not dissociating during the oscillation cycle. Hence, NanoSPR is an attractive label-free real-time technique for the local investigation of molecular dynamics with high observation bandwidth. It gives access to systems, which cannot be fluorescently labeled, and resolves local dynamics that would average out over the sensor area used in conventional SPR"],["dc.identifier.doi","10.1021/acs.nanolett.6b00507"],["dc.identifier.isi","000377642700019"],["dc.identifier.pmid","27172130"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40451"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.title","Single Particle Plasmon Sensors as Label-Free Technique To Monitor MinDE Protein Wave Propagation on Membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2216"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2228"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Savic, Filip"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Verbeek, Sarah"],["dc.contributor.author","Bao, Chunxiao"],["dc.contributor.author","Thiart, Jan"],["dc.contributor.author","Kros, Alexander"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:14:08Z"],["dc.date.available","2018-11-07T10:14:08Z"],["dc.date.issued","2016"],["dc.description.abstract","The fusion of lipid membranes is a key process in biology. It enables cells and organelles to exchange molecules with their surroundings, which otherwise could not cross the membrane barrier. To study such complex processes we use simplified artificial model systems, i.e., an optical fusion assay based on membrane-coated glass spheres. We present a technique to analyze membrane-membrane interactions in a large ensemble of particles. Detailed information on the geometry of the fusion stalk of fully fused membranes is obtained by studying the diffusional lipid dynamics with fluorescence recovery after photo-bleaching experiments. A small contact zone is a strong obstruction for the particle exchange across the fusion spot. With the aid of computer simulations, fluorescence-recovery-after-photobleaching recovery times of both fused and single-membrane-coated beads allow us to estimate the size of the contact zones between two membrane-coated beads. Minimizing delamination and bending energy leads to minimal angles close to those geometrically allowed."],["dc.description.sponsorship","SFB [803 (B08)]"],["dc.identifier.doi","10.1016/j.bpj.2016.04.026"],["dc.identifier.isi","000376436700012"],["dc.identifier.pmid","27224487"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40569"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Geometry of the Contact Zone between Fused Membrane-Coated Beads Mimicking Cell-Cell Fusion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","77a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Kubsch, Bastian"],["dc.contributor.author","Robinson, Tom"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Lipowsky, Reinhard"],["dc.contributor.author","Dimova, Rumiana"],["dc.date.accessioned","2020-12-10T14:22:43Z"],["dc.date.available","2020-12-10T14:22:43Z"],["dc.date.issued","2017"],["dc.format.extent","77A"],["dc.identifier.doi","10.1016/j.bpj.2016.11.462"],["dc.identifier.isi","000402328000396"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71707"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","New Orleans, LA"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Phase Specific Membrane Fusion with SNARE Mimetics"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","608a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","114"],["dc.contributor.author","Robinson, Tom"],["dc.contributor.author","Kubsch, Bastian"],["dc.contributor.author","Kliesch, Torben"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Lipowsky, Reinhard"],["dc.contributor.author","Dimova, Rumiana"],["dc.date.accessioned","2020-12-10T14:22:44Z"],["dc.date.available","2020-12-10T14:22:44Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.bpj.2017.11.3323"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71715"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Spatially Confined Membrane Fusion with SNARE Mimetics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","19953"],["dc.bibliographiccitation.issue","38"],["dc.bibliographiccitation.journal","The Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","19961"],["dc.bibliographiccitation.volume","291"],["dc.contributor.author","Gleisner, Martin"],["dc.contributor.author","Kroppen, Benjamin"],["dc.contributor.author","Fricke, Christian"],["dc.contributor.author","Teske, Nelli"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Meinecke, Michael"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2020-12-10T18:12:56Z"],["dc.date.available","2020-12-10T18:12:56Z"],["dc.date.issued","2016"],["dc.description.abstract","The epsin N-terminal homology domain (ENTH) is a major player in clathrin-mediated endocytosis. To investigate the influence of initial membrane tension on ENTH binding and activity, we established a bilayer system based on adhered giant unilamellar vesicles (GUVs) to be able to control and adjust the membrane tension sigma covering a broad regime. The shape of each individual adhered GUV as well as its adhesion area was monitored by spinning disc confocal laser microscopy. Control of sigma in a range of 0.08-1.02 mN/m was achieved by altering the Mg2+ concentration in solution, which changes the surface adhesion energy per unit area of the GUVs. Specific binding of ENTH to phosphatidylinositol 4,5-bisphosphate leads to a substantial increase in adhesion area of the sessile GUV. At low tension (<0.1 mN/m) binding of ENTH can induce tubular structures, whereas at higher membrane tension the ENTH interaction deflates the sessile GUV and thereby increases the adhesion area. The increase in adhesion area is mainly attributed to a decrease in the area compressibility modulus K-A. We propose that the insertion of the ENTH helix-0 into the membrane is largely responsible for the observed decrease in K-A, which is supported by the observation that the mutant ENTH L6E shows a reduced increase in adhesion area. These results demonstrate that even in the absence of tubule formation, the area compressibility modulus and, as such, the bending rigidity of the membrane is considerably reduced upon ENTH binding. This renders membrane bending and tubule formation energetically less costly."],["dc.identifier.doi","10.1074/jbc.M116.731612"],["dc.identifier.eissn","1083-351X"],["dc.identifier.gro","3141621"],["dc.identifier.isi","000383243100019"],["dc.identifier.issn","0021-9258"],["dc.identifier.pmid","27466364"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74538"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1083-351X"],["dc.relation.issn","0021-9258"],["dc.relation.orgunit","Institut für Zellbiochemie"],["dc.title","Epsin N-terminal Homology Domain (ENTH) Activity as a Function of Membrane Tension"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Bao, Chunxiao"],["dc.contributor.author","Savic, Filip"],["dc.contributor.author","Kliesch, T.-T."],["dc.contributor.author","Verbeek, Sarah"],["dc.contributor.author","Paehler, Gesa"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:55:30Z"],["dc.date.available","2018-11-07T09:55:30Z"],["dc.date.issued","2015"],["dc.format.extent","S189"],["dc.identifier.isi","000380001400561"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36756"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Dresden, GERMANY"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Optical fusion assay based on membrane-coated spheres in a 2D assembly"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","57"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Colloid and Interface Science"],["dc.bibliographiccitation.lastpage","63"],["dc.bibliographiccitation.volume","366"],["dc.contributor.author","Lazzara, Thomas D."],["dc.contributor.author","Behn, Daniela"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:49:01Z"],["dc.date.available","2017-09-07T11:49:01Z"],["dc.date.issued","2012"],["dc.description.abstract","Anodic aluminum oxide (AAO) substrates with aligned, cylindrical, non-intersecting pores with diameters of 75 nm and depths of 3.5 or 10 mu m were functionalized with lipid monolayers harboring different receptor lipids. AAO was first functionalized with dodecyl-trichlorosilane, followed by fusion of small unilamellar vesicles (SUVs) forming a lipid monolayer. The SUVs' lipid composition was transferred onto the AAO surface, allowing us to control the surface receptor density. Owing to the optical transparency of the MO, the overall vesicle spreading process and subsequent protein binding to the receptor-doped lipid monolayers could be investigated in situ by optical waveguide spectroscopy (OWS). SUV spreading occurred at the pore-rim interface, followed by lateral diffusion of lipids within the pore-interior surface until homogeneous coverage was achieved with a lipid monolayer. The functionality of the system was demonstrated through streptavidin binding onto a biotin-DOPE containing POPC membrane, showing maximum protein coverage at 10 mol% of biotin-DOPE. The system enabled us to monitor in real-time the selective extraction of two histidine-tagged proteins, PIGEA14 (14 kDa) and ezrin (70 kDa), directly from cell lysate solutions using a DOGS-NTA(Ni)/DOPC (1:9) membrane. The purification process including protein binding and elution was monitored by OWS and confirmed by SOS-PAGE. (C) 2011 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.jcis.2011.09.067"],["dc.identifier.gro","3142589"],["dc.identifier.isi","000297385900009"],["dc.identifier.pmid","22033154"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8956"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0021-9797"],["dc.title","Phospholipids as an alternative to direct covalent coupling: Surface functionalization of nanoporous alumina for protein recognition and purification"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","477"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","MHR Basic science of reproductive medicine"],["dc.bibliographiccitation.lastpage","488"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Aeckerle, Nelia"],["dc.contributor.author","Eildermann, K."],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Ehmcke, J."],["dc.contributor.author","Schweyer, Stefan"],["dc.contributor.author","Lerchl, A."],["dc.contributor.author","Bergmann, M."],["dc.contributor.author","Kliesch, S."],["dc.contributor.author","Gromoll, Joerg"],["dc.contributor.author","Schlatt, S."],["dc.contributor.author","Behr, R."],["dc.date.accessioned","2018-11-07T09:05:18Z"],["dc.date.available","2018-11-07T09:05:18Z"],["dc.date.issued","2012"],["dc.description.abstract","Mammalian spermatogenesis is maintained by spermatogonial stem cells (SSCs). However, since evidentiary assays and unequivocal markers are still missing in non-human primates (NHPs) and man, the identity of primate SSCs is unknown. In contrast, in mice, germ cell transplantation studies have functionally demonstrated the presence of SSCs. LIN28 is an RNA-binding pluripotent stem cell factor, which is also strongly expressed in undifferentiated mouse spermatogonia. By contrast, two recent reports indicated that LIN28 is completely absent from adult human testes. Here, we analyzed LIN28 expression in marmoset monkey (Callithrix jacchus) and human testes during development and adulthood and compared it with that in mice. In the marmoset, LIN28 was strongly expressed in migratory primordial germ cells and gonocytes. Strikingly, we found a rare LIN28-positive subpopulation of spermatogonia also in adult marmoset testis. This was corroborated by western blotting and quantitative RTPCR. Importantly, in contrast to previous publications, we found LIN28-positive spermatogonia also in normal adult human and additional adult NHP testes. Some seasonal breeders exhibit a degenerated (involuted) germinal epithelium consisting only of Sertoli cells and SSCs during their non-breeding season. The latter re-initiate spermatogenesis prior to the next breeding-season. Fully involuted testes from a seasonal hamster and NHP (Lemur catta) exhibited numerous LIN28-positive spermatogonia, indicating an SSC identity of the labeled cells. We conclude that LIN28 is differentially expressed in mouse and NHP spermatogonia and might be a marker for a rare SSC population in NHPs and man. Further characterization of the LIN28-positive population is required."],["dc.identifier.doi","10.1093/molehr/gas025"],["dc.identifier.isi","000309463100002"],["dc.identifier.pmid","22689537"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25285"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1460-2407"],["dc.relation.issn","1360-9947"],["dc.title","The pluripotency factor LIN28 in monkey and human testes: a marker for spermatogonial stem cells?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]