Kliesch, Torben-Tobias

[["dc.bibliographiccitation.firstpage","206"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cells Tissues Organs"],["dc.bibliographiccitation.lastpage","220"],["dc.bibliographiccitation.volume","196"],["dc.contributor.author","Eildermann, K."],["dc.contributor.author","Aeckerle, Nelia"],["dc.contributor.author","Debowski, Katharina"],["dc.contributor.author","Godmann, M."],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Heistermann, Michael"],["dc.contributor.author","Schweyer, Stefan"],["dc.contributor.author","Bergmann, M."],["dc.contributor.author","Kliesch, S."],["dc.contributor.author","Gromoll, Joerg"],["dc.contributor.author","Ehmcke, J."],["dc.contributor.author","Schlatt, S."],["dc.contributor.author","Behr, R."],["dc.date.accessioned","2018-11-07T09:14:59Z"],["dc.date.available","2018-11-07T09:14:59Z"],["dc.date.issued","2012"],["dc.description.abstract","SALL4 (sal-like protein 4) is a pluripotency transcription factor, which is highly expressed in embryonic stem (ES) cells and which is essential for mouse preimplantation development. In adult mouse organs, Sall4 mRNA is highly expressed in the testis and ovary, while there is only little or no expression in other organs. There is also a high expression of SALL4 in human testicular germ cell tumors. However, there is as yet no detailed analysis of SALL4 expression during mammalian testicular development. We analyzed SALL4 expression in ES cells, preimplantation embryos, and the developing and adult testis of a nonhuman primate (NHP) species, the common marmoset monkey (Callithrix jacchus). Immunofluorescence revealed SALL4 in the nuclei of marmoset ES cells and preimplantation embryos. Marmoset SALL4 isoform analysis in ES cells and newborn and adult testis by RTPCR and Western blotting showed two different isoforms, SALL4-A and SALL4-B. Immunohistochemistry localized this transcription factor to the nuclei of primordial germ cells and most gonocytes in the prenatal and early postnatal marmoset testis. In the pubertal and adult testis SALL4 was present in undifferentiated spermatogonia. In the developing and adult human and mouse testis SALL4 expression mimicked the pattern in the marmoset. Adult testes from additional NHP species, the treeshrew, the cat and the dog also exhibited SALL4 in undifferentiated spermatogonia, indicating a conserved expression in the mammalian testis. Taking into account the importance of SALL4 for mouse development, we conclude that SALL4 may play an important role during mammalian germ cell development and is involved in the regulation of spermatogonial proliferation in the adult testis. Copyright (C) 2012 S. Karger AG, Basel"],["dc.identifier.doi","10.1159/000335031"],["dc.identifier.isi","000308266400002"],["dc.identifier.pmid","22572102"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9081"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27561"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.relation.issn","1422-6405"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Developmental Expression of the Pluripotency Factor Sal-Like Protein 4 in the Monkey, Human and Mouse Testis: Restriction to Premeiotic Germ Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4487"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Soft Matter"],["dc.bibliographiccitation.lastpage","4495"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Schaefer, Edith"],["dc.contributor.author","Vache, Marian"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:03:13Z"],["dc.date.available","2018-11-07T10:03:13Z"],["dc.date.issued","2015"],["dc.description.abstract","Indentation of giant liposomes with a conical indenter is described by means of a tension-based membrane model. We found that nonlinear membrane theory neglecting the impact of bending sufficiently describes the mechanical response of liposomes to indentation as measured by atomic force microscopy. Giant vesicles are gently adsorbed on glassy surfaces via avidin-biotin linkages and indented centrally using an atomic force microscope equipped with conventional sharp tips mounted on top of an inverted microscope. Force indentation curves display a nonlinear response that allows to extract pre-stress of the bilayer T-0 and the area compressibility modulus K-A by computing the contour of the vesicle at a given force. The values for K-A of fluid membranes correspond well to what is known from micropipet suction experiments and inferred from membrane undulation monitoring. Assembly of actin shells inside the liposome considerably stiffens the vesicles resulting in significantly larger area compressibility modules. The analysis can be easily extended to different indenter geometries with rotational symmetry."],["dc.description.sponsorship","[SFB 803 (B08)]"],["dc.identifier.doi","10.1039/c5sm00191a"],["dc.identifier.isi","000355555100018"],["dc.identifier.pmid","25946988"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12612"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38409"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","1744-6848"],["dc.relation.issn","1744-683X"],["dc.rights.access","openAccess"],["dc.title","Mechanical response of adherent giant liposomes to indentation with a conical AFM-tip"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1068"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","ACS Applied Materials & Interfaces"],["dc.bibliographiccitation.lastpage","1076"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Lazzara, Thomas D."],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:44:17Z"],["dc.date.available","2017-09-07T11:44:17Z"],["dc.date.issued","2011"],["dc.description.abstract","Anodic aluminum oxide (AAO) membranes with aligned, cylindrical, nonintersecting pores were selectively fiinctionalized in order to create dual-functionality substrates with different pore-rim and pore-interior surface functionalities, using silane chemistry. We used a two-step process involving an evaporated thin gold film to protect the underlying surface functionality of the pore rims. Subsequent treatment with oxygen plasma of the modified AAO membrane removed the unprotected organic functional groups, i.e., the pore-interior surface. After gold removal, the substrate became optically transparent, and displayed two distinct surface functionalities, one at the pore-rim surface and another at the pore-interior surface. We achieved a selective hydrophobic functionalization with dodecyl-trichlorosilane of either the pore rims or the pore interiors. The deposition of planar lipid membranes on the functionalized areas by addition of small unilamellar vesicles occurred in a predetermined fashion. Small unilamellar vesicles only ruptured upon contact with the hydrophobic substrate regions forming solid supported hybrid bilayers. In addition, pore-rim functionalization with dodecyl-trichlorosilane allowed the formation of pore-spanning hybrid lipid membranes as a result of giant unilamellar vesicle rupture. Confocal laser scanning microscopy was employed to identify the selective spatial localization of the adsorbed fluorescently labeled lipids. The corresponding increase in the AAO refractive index due to lipid adsorption on the hydrophobic regions was monitored by optical waveguide spectroscopy. This simple orthogonal functionalization route is a promising method to control the three-dimensional surface functionality of nanoporous films."],["dc.identifier.doi","10.1021/am101212h"],["dc.identifier.gro","3142745"],["dc.identifier.isi","000289762400021"],["dc.identifier.pmid","21370818"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9425"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/183"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1944-8244"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Orthogonal Functionalization of Nanoporous Substrates: Control of 3D Surface Functionality"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","12070"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific reports"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Dietz, Jörn"],["dc.contributor.author","Turco, Laura"],["dc.contributor.author","Halder, Partho"],["dc.contributor.author","Polo, Elena"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2019-07-09T11:44:35Z"],["dc.date.available","2019-07-09T11:44:35Z"],["dc.date.issued","2017-09-21"],["dc.description.abstract","The large gap in time scales between membrane fusion occurring in biological systems during neurotransmitter release and fusion observed between model membranes has provoked speculations over a large number of possible factors that might explain this discrepancy. One possible reason is an elevated lateral membrane tension present in the presynaptic membrane. We investigated the tension-dependency of fusion using model membranes equipped with a minimal fusion machinery consisting of syntaxin 1, synaptobrevin and SNAP 25. Two different strategies were realized; one based on supported bilayers and the other one employing sessile giant liposomes. In the first approach, isolated patches of planar bilayers derived from giant unilamellar vesicles containing syntaxin 1 and preassembled SNAP 25 (ΔN-complex) were deposited on a dilatable PDMS sheet. In a second approach, lateral membrane tension was controlled through the adhesion of intact giant unilamellar vesicles on a functionalized surface. In both approaches fusion efficiency increases considerably with lateral tension and we identified a threshold tension of 3.4 mN m(-1), at which the number of fusion events is increased substantially."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.1038/s41598-017-12348-w"],["dc.identifier.pmid","28935937"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14824"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59040"],["dc.language.iso","en"],["dc.relation.issn","2045-2322"],["dc.rights.access","openAccess"],["dc.subject.ddc","540"],["dc.title","Membrane tension increases fusion efficiency of model membranes in the presence of SNAREs."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]