Gleisner, Martin

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Gleisner, M."],["dc.contributor.author","Dreker, C."],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Meinecke, Michael"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-11-07T09:22:32Z"],["dc.date.available","2018-11-07T09:22:32Z"],["dc.date.issued","2013"],["dc.format.extent","S122"],["dc.identifier.isi","000330215300334"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29365"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Lisbon, PORTUGAL"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.relation.orgunit","Institut für Zellbiochemie"],["dc.title","Pore spanning membranes as a model system for the selective generation of membrane curvature"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14175"],["dc.bibliographiccitation.issue","49"],["dc.bibliographiccitation.journal","Langmuir : the ACS journal of surfaces and colloids"],["dc.bibliographiccitation.lastpage","14183"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Teske, Nelli"],["dc.contributor.author","Sibold, Jeremias"],["dc.contributor.author","Schumacher, Johannes"],["dc.contributor.author","Teiwes, Nikolas K."],["dc.contributor.author","Gleisner, Martin"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-01-17T13:00:51Z"],["dc.date.available","2018-01-17T13:00:51Z"],["dc.date.issued","2017"],["dc.description.abstract","A number of techniques has been developed and analyzed in recent years to generate pore-spanning membranes (PSMs). While quite a number of methods rely on nanoporous substrates, only a few use micrometer-sized pores to be able to individually resolve suspending membranes by means of fluorescence microscopy. To be able to produce PSMs on pores that are micrometer in size, an orthogonal functionalization strategy resulting in a hydrophilic surface is highly desirable. Here, we report on a method to prepare PSMs based on the evaporation of a thin layer of silicon monoxide on top of the porous substrate. PM-IRRAS experiments demonstrate that the final surface is composed of SiOx with 1 < x < 2. The hydrophilic surface turned out to be well suited to spread giant unilamellar vesicles forming PSMs. As the method does not rely on a gold coating as frequently used for orthogonal functionalization, fluorescence micrographs provide information not only from the freestanding membrane areas but also from the supported ones. The observation of the entire PSM area enabled us to observe phase-separation in these membranes on the freestanding and supported parts as well as protein binding and possible lipid reorganization of the membranes induced by binding of the protein Shiga toxin."],["dc.identifier.doi","10.1021/acs.langmuir.7b02727"],["dc.identifier.pmid","29148811"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11698"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1520-5827"],["dc.title","Continuous Pore-Spanning Lipid Bilayers on Silicon Oxide-Coated Porous Substrates"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6228"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","Soft Matter"],["dc.bibliographiccitation.lastpage","6236"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Gleisner, Martin"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Barbot, Mariam"],["dc.contributor.author","Dreker, Christina"],["dc.contributor.author","Meinecke, Michael"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:46:54Z"],["dc.date.available","2017-09-07T11:46:54Z"],["dc.date.issued","2014"],["dc.description.abstract","The generation of a regular array of micrometre-sized pore-spanning membranes that protrude from the underlying surface as a function of osmotic pressure is reported. Giant unilamellar vesicles are spread onto non-functionalized Si/SiO2 substrates containing a highly ordered array of cavities with pore diameters of 850 nm, an interpore distance of 4 mm and a pore depth of 10 mm. The shape of the resulting pore-spanning membranes is controlled by applying an osmotic pressure difference between the bulk solution and the femtoliter-sized cavity underneath each membrane. By applying Young-Laplace's law assuming moderate lateral membrane tensions, the response of the membranes to the osmotic pressure difference can be theoretically well described. Protruded pore-spanning membranes containing the receptor lipid PIP2 specifically bind the ENTH domain of epsin resulting in an enlargement of the protrusions and disappearance as a result of ENTH-domain induced defects in the membranes. These results are discussed in the context of an ENTH-domain induced reduction of lateral membrane tension and formation of defects as a result of helix insertion of the protein in the bilayer."],["dc.identifier.doi","10.1039/c4sm00702f"],["dc.identifier.fs","606035"],["dc.identifier.gro","3142206"],["dc.identifier.isi","000340438600011"],["dc.identifier.pmid","25012509"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11470"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5710"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: DFG [SFB 803]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1744-6848"],["dc.relation.issn","1744-683X"],["dc.relation.orgunit","Institut für Zellbiochemie"],["dc.rights.access","openAccess"],["dc.title","Driving a planar model system into the 3rd dimension: generation and control of curved pore-spanning membrane arrays"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Gleisner, M."],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Barbot, M."],["dc.contributor.author","Dreker, C."],["dc.contributor.author","Meinecke, Michael"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-11-07T09:55:32Z"],["dc.date.available","2018-11-07T09:55:32Z"],["dc.date.issued","2015"],["dc.format.extent","S78"],["dc.identifier.isi","000380001400135"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36765"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New York"],["dc.relation.eventlocation","Dresden"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.relation.orgunit","Institut für Zellbiochemie"],["dc.title","Impact of the ENTH domain on protruded pore spanning membranes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]