Gleisner, Martin

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Gleisner, M."],["dc.contributor.author","Dreker, C."],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Meinecke, Michael"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-11-07T09:22:32Z"],["dc.date.available","2018-11-07T09:22:32Z"],["dc.date.issued","2013"],["dc.format.extent","S122"],["dc.identifier.isi","000330215300334"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29365"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Lisbon, PORTUGAL"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.relation.orgunit","Institut für Zellbiochemie"],["dc.title","Pore spanning membranes as a model system for the selective generation of membrane curvature"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14175"],["dc.bibliographiccitation.issue","49"],["dc.bibliographiccitation.journal","Langmuir : the ACS journal of surfaces and colloids"],["dc.bibliographiccitation.lastpage","14183"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Teske, Nelli"],["dc.contributor.author","Sibold, Jeremias"],["dc.contributor.author","Schumacher, Johannes"],["dc.contributor.author","Teiwes, Nikolas K."],["dc.contributor.author","Gleisner, Martin"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-01-17T13:00:51Z"],["dc.date.available","2018-01-17T13:00:51Z"],["dc.date.issued","2017"],["dc.description.abstract","A number of techniques has been developed and analyzed in recent years to generate pore-spanning membranes (PSMs). While quite a number of methods rely on nanoporous substrates, only a few use micrometer-sized pores to be able to individually resolve suspending membranes by means of fluorescence microscopy. To be able to produce PSMs on pores that are micrometer in size, an orthogonal functionalization strategy resulting in a hydrophilic surface is highly desirable. Here, we report on a method to prepare PSMs based on the evaporation of a thin layer of silicon monoxide on top of the porous substrate. PM-IRRAS experiments demonstrate that the final surface is composed of SiOx with 1 < x < 2. The hydrophilic surface turned out to be well suited to spread giant unilamellar vesicles forming PSMs. As the method does not rely on a gold coating as frequently used for orthogonal functionalization, fluorescence micrographs provide information not only from the freestanding membrane areas but also from the supported ones. The observation of the entire PSM area enabled us to observe phase-separation in these membranes on the freestanding and supported parts as well as protein binding and possible lipid reorganization of the membranes induced by binding of the protein Shiga toxin."],["dc.identifier.doi","10.1021/acs.langmuir.7b02727"],["dc.identifier.pmid","29148811"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11698"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1520-5827"],["dc.title","Continuous Pore-Spanning Lipid Bilayers on Silicon Oxide-Coated Porous Substrates"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Gleisner, M."],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Barbot, M."],["dc.contributor.author","Dreker, C."],["dc.contributor.author","Meinecke, Michael"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-11-07T09:55:32Z"],["dc.date.available","2018-11-07T09:55:32Z"],["dc.date.issued","2015"],["dc.format.extent","S78"],["dc.identifier.isi","000380001400135"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36765"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New York"],["dc.relation.eventlocation","Dresden"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.relation.orgunit","Institut für Zellbiochemie"],["dc.title","Impact of the ENTH domain on protruded pore spanning membranes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","19953"],["dc.bibliographiccitation.issue","38"],["dc.bibliographiccitation.journal","The Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","19961"],["dc.bibliographiccitation.volume","291"],["dc.contributor.author","Gleisner, Martin"],["dc.contributor.author","Kroppen, Benjamin"],["dc.contributor.author","Fricke, Christian"],["dc.contributor.author","Teske, Nelli"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Meinecke, Michael"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2020-12-10T18:12:56Z"],["dc.date.available","2020-12-10T18:12:56Z"],["dc.date.issued","2016"],["dc.description.abstract","The epsin N-terminal homology domain (ENTH) is a major player in clathrin-mediated endocytosis. To investigate the influence of initial membrane tension on ENTH binding and activity, we established a bilayer system based on adhered giant unilamellar vesicles (GUVs) to be able to control and adjust the membrane tension sigma covering a broad regime. The shape of each individual adhered GUV as well as its adhesion area was monitored by spinning disc confocal laser microscopy. Control of sigma in a range of 0.08-1.02 mN/m was achieved by altering the Mg2+ concentration in solution, which changes the surface adhesion energy per unit area of the GUVs. Specific binding of ENTH to phosphatidylinositol 4,5-bisphosphate leads to a substantial increase in adhesion area of the sessile GUV. At low tension (<0.1 mN/m) binding of ENTH can induce tubular structures, whereas at higher membrane tension the ENTH interaction deflates the sessile GUV and thereby increases the adhesion area. The increase in adhesion area is mainly attributed to a decrease in the area compressibility modulus K-A. We propose that the insertion of the ENTH helix-0 into the membrane is largely responsible for the observed decrease in K-A, which is supported by the observation that the mutant ENTH L6E shows a reduced increase in adhesion area. These results demonstrate that even in the absence of tubule formation, the area compressibility modulus and, as such, the bending rigidity of the membrane is considerably reduced upon ENTH binding. This renders membrane bending and tubule formation energetically less costly."],["dc.identifier.doi","10.1074/jbc.M116.731612"],["dc.identifier.eissn","1083-351X"],["dc.identifier.gro","3141621"],["dc.identifier.isi","000383243100019"],["dc.identifier.issn","0021-9258"],["dc.identifier.pmid","27466364"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74538"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1083-351X"],["dc.relation.issn","0021-9258"],["dc.relation.orgunit","Institut für Zellbiochemie"],["dc.title","Epsin N-terminal Homology Domain (ENTH) Activity as a Function of Membrane Tension"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]