Brenig, Bertram B.

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Granados-Soler, José Luis"],["dc.contributor.author","Taher, Leila"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Bornemann-Kolatzki, Kirsten"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Nerschbach, Verena"],["dc.contributor.author","Ferreira, Fernando"],["dc.contributor.author","Junginger, Johannes"],["dc.contributor.author","Hewicker-Trautwein, Marion"],["dc.contributor.author","Murua Escobar, Hugo"],["dc.contributor.author","Nolte, Ingo"],["dc.date.accessioned","2022-11-01T10:16:44Z"],["dc.date.available","2022-11-01T10:16:44Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\n \n The molecular heterogeneity of feline mammary carcinomas (FMCs) represents a prognostic and therapeutic challenge. RNA-Seq-based comparative transcriptomic profiling serves to identify recurrent and exclusive differentially expressed genes (DEGs) across sample types and molecular subtypes. Using mass-parallel RNA-Seq, we identified DEGs and performed comparative function-based analysis across 15 tumours (four basal-like triple-negative [TN], eight normal-like TN, and three luminal B\n f\n HER2 negative [LB\n f\n HER2−]), two cell lines (CL, TiHo-0906, and TiHo-1403) isolated from the primary tumours (LB\n f\n HER2−) of two cats included in this study, and 13 healthy mammary tissue controls. DEGs in tumours were predominantly upregulated; dysregulation of CLs transcriptome was more extensive, including mostly downregulated genes. Cell-cycle and metabolic-related DEGs were upregulated in both tumours and CLs, including therapeutically-targetable cell cycle regulators (e.g.\n CCNB1\n ,\n CCNB2\n ,\n CDK1\n ,\n CDK4\n ,\n GTSE1\n ,\n MCM4\n , and\n MCM5)\n , metabolic-related genes (e.g.\n FADS2\n and\n SLC16A3\n ), heat-shock proteins (e.g.\n HSPH1, HSP90B1\n , and\n HSPA5\n ), genes controlling centrosome disjunction (e.g.\n RACGAP1\n and\n NEK2\n ), and collagen molecules (e.g.\n COL2A1\n ). DEGs specifically upregulated in basal-like TN tumours were involved in antigen processing and presentation, in normal-like TN tumours encoded G protein-coupled receptors (GPCRs), and in LB\n f\n HER2− tumours were associated with lysosomes, phagosomes, and endosomes formation. Downregulated DEGs in CLs were associated with structural and signalling cell surface components. Hence, our results suggest that upregulation of genes enhancing proliferation and metabolism is a common feature among FMCs and derived CLs. In contrast, the dissimilarities observed in dysregulation of membrane components highlight CLs’ disconnection with the tumour microenvironment. Furthermore, recurrent and exclusive DEGs associated with dysregulated pathways might be useful for the development of prognostically and therapeutically-relevant targeted panels."],["dc.identifier.doi","10.1038/s41598-022-20874-5"],["dc.identifier.pii","20874"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116639"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-605"],["dc.relation.eissn","2045-2322"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Transcription profiling of feline mammary carcinomas and derived cell lines reveals biomarkers and drug targets associated with metabolic and cell cycle pathways"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Granados-Soler, José Luis"],["dc.contributor.author","Bornemann-Kolatzki, Kirsten"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.author","Betz, Daniela"],["dc.contributor.author","Junginger, Johannes"],["dc.contributor.author","Hewicker-Trautwein, Marion"],["dc.contributor.author","Murua Escobar, Hugo"],["dc.contributor.author","Nolte, Ingo"],["dc.date.accessioned","2021-04-14T08:27:35Z"],["dc.date.available","2021-04-14T08:27:35Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1038/s41598-020-57942-7"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82340"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2045-2322"],["dc.title","Analysis of Copy-Number Variations and Feline Mammary Carcinoma Survival"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]