Brenig, Bertram B.

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","BMC genetics"],["dc.bibliographiccitation.lastpage","4"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Pfeiffer, I."],["dc.contributor.author","Brenig, B."],["dc.date.accessioned","2019-07-09T11:52:30Z"],["dc.date.available","2019-07-09T11:52:30Z"],["dc.date.issued","2005"],["dc.description.abstract","Background: Simple and precise methods for sex determination in animals are a pre-requisite for a number of applications in animal production and forensics. However, some of the existing methods depend only on the detection of Y-chromosome specific sequences. Therefore, the abscence of a signal does not necessarily mean that the sample is of female origin, because experimental errors can also lead to negative results. Thus, the detection of Y- and X-chromosome specific sequences is advantageous. Results: A novel method for sex identification in mammals (sheep, Ovis aries and European red deer, Cervus elaphus) is described, using a polymerase chain reaction (PCR) and sequencing of a part of the amelogenin gene. A partial sequence of the amelogenin gene of sheep and red deer was obtained, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. With a specific pair of primers a DNA fragment of different length between the male and female mammal was amplified."],["dc.identifier.doi","10.1186/1471-2156-6-16"],["dc.identifier.fs","28009"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/4420"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60205"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1471-2156"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","590"],["dc.title","X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (Ovis aries) and European red deer (Cervus elaphus)"],["dc.title.alternative","methodology article"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]