Brenig, Bertram B.

[["dc.bibliographiccitation.firstpage","161"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Bacteria"],["dc.bibliographiccitation.lastpage","182"],["dc.bibliographiccitation.volume","1"],["dc.contributor.affiliation","Valdez-Baez, Juan; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","da Costa, Francielly Morais Rodrigues; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","Pinto Gomide, Anne Cybelle; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","Profeta, Rodrigo; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","da Silva, Alessandra Lima; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","Sousa, Thiago de Jesus; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","Viana, Marcus Vinícius Canário; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","Bentes Kato, Rodrigo; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","Americo, Monique Ferrary; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","dos Santos Freitas, Andria; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","Carvalho, Rodrigo Dias de Oliveira; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.affiliation","Brenig, Bertram; 3Institute of Veterinary Medicine, University of Göttingen, Burckhardtweg 2, 37077 Göttingen, Germany; bbrenig@gwdg.de"],["dc.contributor.affiliation","Martins, Flaviano Santos; 4Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; flaviano@icb.ufmg.br"],["dc.contributor.affiliation","Aburjaile, Flavia; 5Department of Preventive Veterinary Medicine, Veterinary School, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"],["dc.contributor.affiliation","Azevedo, Vasco; 1Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; juanlvaldezb@gmail.com (J.V.-B.); franrodriguesdacosta@gmail.com (F.M.R.d.C.); acybelle@gmail.com (A.C.P.G.); profeta.biotec@gmail.com (R.P.); alessandralima92@gmail.com (A.L.d.S.); thiagojsousa@gmail.com (T.d.J.S.); canarioviana@gmail.com (M.V.C.V.); rbkato@gmail.com (R.B.K.); moniquefamerico@gmail.com (M.F.A.); andria.sfreitas@gmail.com (A.d.S.F.); rodrigodoc2@gmail.com (R.D.d.O.C.)"],["dc.contributor.author","Valdez-Baez, Juan"],["dc.contributor.author","da Costa, Francielly Morais Rodrigues"],["dc.contributor.author","Pinto Gomide, Anne Cybelle"],["dc.contributor.author","Profeta, Rodrigo"],["dc.contributor.author","da Silva, Alessandra Lima"],["dc.contributor.author","Sousa, Thiago de Jesus"],["dc.contributor.author","Viana, Marcus Vinícius Canário"],["dc.contributor.author","Bentes Kato, Rodrigo"],["dc.contributor.author","Americo, Monique Ferrary"],["dc.contributor.author","dos Santos Freitas, Andria"],["dc.contributor.author","Carvalho, Rodrigo Dias de Oliveira"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Martins, Flaviano Santos"],["dc.contributor.author","Aburjaile, Flavia"],["dc.contributor.author","Azevedo, Vasco"],["dc.date.accessioned","2022-09-05T08:55:09Z"],["dc.date.available","2022-09-05T08:55:09Z"],["dc.date.issued","2022-07-13"],["dc.date.updated","2022-09-04T02:42:38Z"],["dc.description.abstract","Bifidobacterium breve is among the first microorganisms colonizing the intestinal tract in humans and is a predominant species in the gut microbiota of newborns and children. This bacterium is widely used in the probiotic industry due to its capacity to improve host health. The search for new targets with probiotic properties is an increasing trend with the help of next-generation sequencing as they facilitate the characterization of the bacterial features. B. breve 1101A was isolated from the faeces of healthy children in Brazil and therefore could play a protective role in the gut. To investigate the beneficial properties of this strain, the present study performed a comprehensive characterization of the genetic features involved in the bacterium resistance and adaptation to gastrointestinal conditions, production of nutrients, and immunomodulatory compounds. Furthermore, this study carried out the prediction of genomic elements (plasmids, prophages, CRISPR-Cas systems, insertion sequences, genomic islands, antibiotic resistance genes) to evaluate the safety of B. breve 1101A. A comparative genomics approach using 45 B. breve complete genomes based on pangenome and phylogenomic analysis was also performed to identify specific genes in B. breve 1101A. The prediction of genetic elements, possibly safety-related, did not detect plasmids, but only one incomplete prophage, two non-functional CRISPR systems, and seven genomic islands. Additionally, three antibiotic resistance genes were identified: ileS (resistance to mupirocin), rpoB, and erm(X). In the comparative genomic analysis, the pangenome was revealed to be open, and B. breve 1101A presented 63 unique genes associated with several processes, such as transmembrane transport, membrane components, DNA processes, and carbohydrate metabolism. In conclusion, B. breve 1101A is potentially safe and well-adapted for intestinal disorder therapeutics, although the role of its unique genetic repertoire needs further investigation."],["dc.identifier.doi","10.3390/bacteria1030013"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114070"],["dc.language.iso","en"],["dc.relation.eissn","2674-1334"],["dc.rights","CC BY 4.0"],["dc.title","Comparative Genomics and In Silico Evaluation of Genes Related to the Probiotic Potential of Bifidobacterium breve 1101A"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0154602"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Wehrhahn, Christin"],["dc.contributor.author","Wanjek, Marius"],["dc.contributor.author","Bortfeld, Ralf"],["dc.contributor.author","Wemheuer, Wilhelm E."],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Brenig, Bertram"],["dc.date.accessioned","2018-11-07T10:15:21Z"],["dc.date.available","2018-11-07T10:15:21Z"],["dc.date.issued","2016"],["dc.description.abstract","Background With the availability of massive SNP data for several economically important cattle breeds, haplotype tests have been performed to identify unknown recessive disorders. A number of so-called lethal haplotypes, have been uncovered in Holstein Friesian cattle and, for at least seven of these, the causative mutations have been identified in candidate genes. However, several lethal haplotypes still remain elusive. Here we report the molecular genetic causes of lethal haplotype 5 (HH5) and cholesterol deficiency (CDH). A targeted enrichment for the known genomic regions, followed by massive parallel sequencing was used to interrogate for causative mutations in a case/control approach. Methods Targeted enrichment for the known genomic regions, followed by massive parallel sequencing was used in a case/control approach. PCRs for the causing mutations were developed and compared to routine imputing in 2,100 (HH5) and 3,100 (CDH) cattle. Results HH5 is caused by a deletion of 138kbp, spanning position 93,233kb to 93,371kb on chromosome 9 (BTA9), harboring only dimethyl-adenosine transferase 1 (TFB1M). The deletion breakpoints are flanked by bovine long interspersed nuclear elements Bov-B (upstream) and L1ME3 (downstream), suggesting a homologous recombination/deletion event. TFB1M di-methylates adenine residues in the hairpin loop at the 3'-end of mitochondrial 12S rRNA, being essential for synthesis and function of the small ribosomal subunit of mitochondria. Homozygous TFB1M(-/-) mice reportedly exhibit embryonal lethality with developmental defects. A 2.8% allelic frequency was determined for the German HF population. CDH results from a 1.3kbp insertion of an endogenous retrovirus (ERV2-1-LTR_BT) into exon 5 of the APOB gene at BTA11: 77,959kb. The insertion is flanked by 6bp target site duplications as described for insertions mediated by retroviral integrases. A premature stop codon in the open reading frame of APOB is generated, resulting in a truncation of the protein to a length of only < 140 amino acids. Such early truncations have been shown to cause an inability of chylomicron excretion from intestinal cells, resulting in malabsorption of cholesterol. The allelic frequency of this mutation in the German HF population was 6.7%, which is substantially higher than reported so far. Compared to PCR assays inferring the genetic variants directly, the routine imputing used so far showed a diagnostic sensitivity of as low as 91% (HH5) and 88% (CDH), with a high specificity for both (>= 99.7%). Conclusion With the availability of direct genetic tests it will now be possible to more effectively reduce the carrier frequency and ultimately eliminate the disorders from the HF populations. Beside this, the fact that repetitive genomic elements (RE) are involved in both diseases, underline the evolutionary importance of RE, which can be detrimental as here, but also advantageous over generations."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.1371/journal.pone.0154602"],["dc.identifier.isi","000375212600046"],["dc.identifier.pmid","27128314"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13249"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40795"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights.access","openAccess"],["dc.title","The Holstein Friesian Lethal Haplotype 5 (HH5) Results from a Complete Deletion of TBF1M and Cholesterol Deficiency (CDH) from an ERV-(LTR) Insertion into the Coding Region of APOB"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","550"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.lastpage","556"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Gordon, Paul M. K."],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Urnovitz, Howard B."],["dc.contributor.author","Graham, Catherine"],["dc.contributor.author","Clark, Renee"],["dc.contributor.author","Dudas, Sandor"],["dc.contributor.author","Czub, Stefanie"],["dc.contributor.author","Sensen, Maria"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Groschup, Martin H."],["dc.contributor.author","Church, Robert B."],["dc.contributor.author","Sensen, Christoph W."],["dc.date.accessioned","2018-11-07T08:33:15Z"],["dc.date.available","2018-11-07T08:33:15Z"],["dc.date.issued","2009"],["dc.description.abstract","To gain insight into the disease progression of transmissible spongiform encephalopathies (TSE), we searched for disease-specific patterns in circulating nucleic acids (CNA) in elk and cattle. In a 25-month time-course experiment, CNAs were isolated from blood samples of 24 elk (Cervus elaphus) orally challenged with chronic wasting disease (CWD) infectious material. In a separate experiment, blood-sample CNAs from 29 experimental cattle (Bos taurus) 40 months post-inoculation with clinical bovine spongiform encephalopathy (BSE) were analyzed according to the same protocol. Next-generation sequencing provided broad elucidation of sample CNAs: we detected infection-specific sequences as early as 11 months in elk (i.e. at least 3 months before the appearance of the first clinical signs) and we established CNA patterns related to BSE in cattle at least 4 months prior to clinical signs. In elk, a progression of CNA sequence patterns was found to precede and correlate with macro-observable disease progression, including delayed CWD progression in elk with PrP genotype LM. Some of the patterns identified contain transcription-factor-binding sites linked to endogenous retroviral integration. These patterns suggest that retroviruses may be connected to the manifestation of TSEs. Our results may become useful for the early diagnosis of TSE in live elk and cattle."],["dc.identifier.doi","10.1093/nar/gkn963"],["dc.identifier.isi","000262963400031"],["dc.identifier.pmid","19059996"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4005"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17531"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","0305-1048"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Disease-specific motifs can be identified in circulating nucleic acids from live elk and cattle infected with transmissible spongiform encephalopathies"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e75485"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Hennecke, Silvia"],["dc.contributor.author","Bornemann-Kolatzki, Kirsten"],["dc.contributor.author","Urnovitz, Howard B."],["dc.contributor.author","Neumann, Stephan"],["dc.contributor.author","Stroebel, Philipp"],["dc.contributor.author","Kaup, Franz-Josef"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Schuetz, Ekkehard"],["dc.date.accessioned","2018-11-07T09:19:45Z"],["dc.date.available","2018-11-07T09:19:45Z"],["dc.date.issued","2013"],["dc.description.abstract","Mammary tumors are the most frequent cancers in female dogs exhibiting a variety of histopathological differences. There is lack of knowledge about the genomes of these common dog tumors. Five tumors of three different histological subtypes were evaluated. Massive parallel sequencing (MPS) was performed in comparison to the respective somatic genome of each animal. Copy number and structural aberrations were validated using droplet digital PCR (ddPCR). Using mate-pair sequencing chromosomal aneuploidies were found in two tumors, frequent smaller deletions were found in one, inter-chromosomal fusions in one other, whereas one tumor was almost normal. These aberrations affect several known cancer associated genes such as cMYC, and KIT. One common deletion of the proximal end of CFA27, harboring the tumor suppressor gene PFDN5 was detected in four tumors. Using ddPCR, this deletion was validated and detected in 50% of tumors (N = 20). Breakpoint specific dPCRs were established for four tumors and tumor specific cell-free DNA (cfDNA) was detected in the plasma. In one animal tumor-specific cfDNA was found >1 year after surgery, attributable to a lung metastasis. Paired-end sequencing proved that copy-number imbalances of the tumor are reflected by the cfDNA. This report on chromosomal instability of canine mammary cancers reveals similarities to human breast cancers as well as special canine alterations. This animal model provides a framework for using MPS for screening for individual cancer biomarkers with cost effective confirmation and monitoring using ddPCR. The possibility exists that ddPCR can be expanded to screening for common cancer related variants."],["dc.identifier.doi","10.1371/journal.pone.0075485"],["dc.identifier.isi","000325423500069"],["dc.identifier.pmid","24098698"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9419"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28716"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Genome Aberrations in Canine Mammary Carcinomas and Their Detection in Cell-Free Plasma DNA"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Sousa, Thiago de Jesus"],["dc.contributor.author","Parise, Doglas"],["dc.contributor.author","Profeta, Rodrigo"],["dc.contributor.author","Parise, Mariana Teixeira Dornelles"],["dc.contributor.author","Gomide, Anne Cybelle Pinto"],["dc.contributor.author","Kato, Rodrigo Bentos"],["dc.contributor.author","Pereira, Felipe Luiz"],["dc.contributor.author","Figueiredo, Henrique Cesar Pereira"],["dc.contributor.author","Ramos, Rommel"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Costa da Silva, Artur Luiz da"],["dc.contributor.author","Ghosh, Preetam"],["dc.contributor.author","Barh, Debmalya"],["dc.contributor.author","Góes-Neto, Aristóteles"],["dc.contributor.author","Azevedo, Vasco"],["dc.date.accessioned","2020-12-10T18:11:08Z"],["dc.date.available","2020-12-10T18:11:08Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1038/s41598-019-52695-4"],["dc.identifier.eissn","2045-2322"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16899"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73904"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Re-sequencing and optical mapping reveals misassemblies and real inversions on Corynebacterium pseudotuberculosis genomes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","35379"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Oncotarget"],["dc.bibliographiccitation.lastpage","35389"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Liu, Wen"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Schmidt, Laura C."],["dc.contributor.author","Roolf, Catrin"],["dc.contributor.author","Pews-Davtyan, Anahit"],["dc.contributor.author","Ruetgen, Barbara C."],["dc.contributor.author","Hammer, Sabine"],["dc.contributor.author","Willenbrock, Saskia"],["dc.contributor.author","Sekora, Anett"],["dc.contributor.author","Rolfs, Arndt"],["dc.contributor.author","Beller, Matthias"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Nolte, Ingo"],["dc.contributor.author","Junghanss, Christian"],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Escobar, Hugo Murua"],["dc.date.accessioned","2018-11-07T10:12:52Z"],["dc.date.available","2018-11-07T10:12:52Z"],["dc.date.issued","2016"],["dc.description.abstract","Protein kinase inhibitors are widely used in chemotherapeutic cancer regimens. Maleimide derivatives such as SB-216763 act as GSK-3 inhibitor targeting cell proliferation, cell death and cell cycle progression. Herein, the two arylindolylmaleimide derivatives PDA-66 and PDA-377 were evaluated as potential chemotherapeutic agents on canine B-cell lymphoma cell lines. Canine lymphoma represents a naturally occurring model closely resembling the human high-grade non-Hodgkin's lymphoma (NHL). PDA-66 showed more pronounced effects on both cell lines. Application of 2.5 mu M PDA-66 resulted in a significant induction of apoptosis (approx. 11 %), decrease of the metabolic activity (approx. 95 %), anti-proliferative effect (approx. 85 %) and cell death within 48h. Agent induced mode of action was characterized by whole transcriptome sequencing, 12 h and 24 h post-agent exposure. Key PDA-66-modulated pathways identified were cell cycle, DNA replication and p53 signaling. Expression analyses indicated that the drug acting mechanism is mediated through DNA replication and cycle arrest involving the spindle assembly checkpoint. In conclusion, both PDA derivatives displayed strong anti-proliferation activity in canine B-cell lymphoma cells. The cell and molecular PDA-induced effect characterization and the molecular characterization of the agent acting mechanism provides the basis for further evaluation of a potential drug for canine lymphoma serving as model for human NHL."],["dc.description.sponsorship","Chinese Scholarship Council (CSC)"],["dc.identifier.doi","10.18632/oncotarget.9297"],["dc.identifier.isi","000377752100139"],["dc.identifier.pmid","27177088"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14133"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40322"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Impact Journals Llc"],["dc.relation.issn","1949-2553"],["dc.rights.access","openAccess"],["dc.title","Characterization of the novel indolylmaleimides' PDA-66 and PDA-377 effect on canine lymphoma cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0129208"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Hardt, Michael"],["dc.contributor.author","Scheuermann, Petra"],["dc.contributor.author","Freick, Markus"],["dc.date.accessioned","2018-11-07T09:55:52Z"],["dc.date.available","2018-11-07T09:55:52Z"],["dc.date.issued","2015"],["dc.description.abstract","Aristaless-like homeobox 4 (ALX4) gene is an important transcription regulator in skull and limb development. In humans and mice ALX4 mutations or loss of function result in a number of skeletal and organ malformations, including polydactyly, tibial hemimelia, omphalocele, biparietal foramina, impaired mammary epithelial morphogenesis, alopecia, coronal craniosynostosis, hypertelorism, depressed nasal bridge and ridge, bifid nasal tip, hypogonadism, and body agenesis. Here we show that a complex skeletal malformation of the hind limb in Galloway cattle together with other developmental anomalies is a recessive autosomal disorder most likely caused by a duplication of 20 bp in exon 2 of the bovine ALX4 gene. A second duplication of 34 bp in exon 4 of the same gene has no known effect, although both duplications result in a frameshift and premature stop codon leading to a truncated protein. Genotyping of 1,688 Black/Red/Belted/Riggit Galloway (GA) and 289 White Galloway (WGA) cattle showed that the duplication in exon 2 has allele frequencies of 1% in GA and 6% in WGA and the duplication in exon 4 has frequencies of 23% in GA and 38% in WGA. Both duplications were not detected in 876 randomly selected German Holstein Friesian and 86 cattle of 21 other breeds. Hence, we have identified a candidate causative mutation for tibial hemimelia syndrome in Galloway cattle and selection against this mutation can be used to eliminate the mutant allele from the breed."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2015"],["dc.identifier.doi","10.1371/journal.pone.0129208"],["dc.identifier.isi","000356329900052"],["dc.identifier.pmid","26076463"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11957"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36844"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights.access","openAccess"],["dc.title","A 20 bp Duplication in Exon 2 of the Aristaless-Like Homeobox 4 Gene (ALX4) Is the Candidate Causative Mutation for Tibial Hemimelia Syndrome in Galloway Cattle"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.affiliation","Jesus, Hendor N. R.; 1Multicenter Post-Graduate Program in Biochemistry and Molecular Biology (PMBqBM), Institute of Health Sciences, Federal University of Bahia, Salvador, BA, Brazil"],["dc.contributor.affiliation","Rocha, Danilo J. P. G.; 2Post-Graduate Program in Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, BA, Brazil"],["dc.contributor.affiliation","Ramos, Rommel T. J.; 3Institute of Biological Sciences, Federal University of Para, Belém, PA, Brazil"],["dc.contributor.affiliation","Silva, Artur; 3Institute of Biological Sciences, Federal University of Para, Belém, PA, Brazil"],["dc.contributor.affiliation","Brenig, Bertram; 4Institute of Veterinary Medicine, University of Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Góes-Neto, Aristóteles; 5Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil"],["dc.contributor.affiliation","Costa, Mateus M.; 6Laboratório de Microbiologia e Imunologia Animal (LAMIA), Universidade Federal Do Vale Do São Francisco, Petrolina, Pernambuco, Brazil"],["dc.contributor.affiliation","Soares, Siomar C.; 7Department of Immunology, Microbiology and Parasitology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro (UFTM), Uberaba, Minas Gerais, Brazil"],["dc.contributor.affiliation","Azevedo, Vasco; 5Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil"],["dc.contributor.affiliation","Aguiar, Eric R. G. R.; 8Department of Biological Sciences, State University of Santa Cruz, Ilhéus, BA, Brazil"],["dc.contributor.affiliation","Martínez-Martínez, Luiz; 9Unidad de Gestión Clínica, Hospital Universitario Reina Sofía, Córdoba, Spain"],["dc.contributor.affiliation","Ocampo, Alain; 12Microbiology Service, University Hospital Marqués de Valdecilla, Santander, Spain"],["dc.contributor.affiliation","Alibi, Sana; 14Research Unit Analysis and Process Applied to the Environment, Rejiche, Tunisia"],["dc.contributor.affiliation","Dorta, Alexis; 13Instituto de Investigación Valdecilla (IDIVAL), Santander, Spain"],["dc.contributor.affiliation","Pacheco, Luis G. C.; 1Multicenter Post-Graduate Program in Biochemistry and Molecular Biology (PMBqBM), Institute of Health Sciences, Federal University of Bahia, Salvador, BA, Brazil"],["dc.contributor.affiliation","Navas, Jesus; 13Instituto de Investigación Valdecilla (IDIVAL), Santander, Spain"],["dc.contributor.author","Jesus, Hendor N. R."],["dc.contributor.author","Rocha, Danilo J. P. G."],["dc.contributor.author","Ramos, Rommel T. J."],["dc.contributor.author","Silva, Artur"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Góes-Neto, Aristóteles"],["dc.contributor.author","Costa, Mateus M."],["dc.contributor.author","Soares, Siomar C."],["dc.contributor.author","Azevedo, Vasco"],["dc.contributor.author","Aguiar, Eric R. G. R."],["dc.contributor.author","Martínez-Martínez, Luiz"],["dc.contributor.author","Ocampo, Alain"],["dc.contributor.author","Alibi, Sana"],["dc.contributor.author","Dorta, Alexis"],["dc.contributor.author","Pacheco, Luis G. C."],["dc.contributor.author","Navas, Jesus"],["dc.date.accessioned","2022-11-30T10:23:29Z"],["dc.date.available","2022-11-30T10:23:29Z"],["dc.date.issued","2022-11-16"],["dc.date.updated","2022-11-30T06:42:54Z"],["dc.description.abstract","Corynebacterium amycolatum is a nonlipophilic coryneform which is increasingly being recognized as a relevant human and animal pathogen showing multidrug resistance to commonly used antibiotics. However, little is known about the molecular mechanisms involved in transition from colonization to the MDR invasive phenotype in clinical isolates. In this study, we performed a comprehensive pan-genomic analysis of C. amycolatum, including 26 isolates from different countries. We obtained the novel genome sequences of 8 of them, which are multidrug resistant clinical isolates from Spain and Tunisia. They were analyzed together with other 18 complete or draft C. amycolatum genomes retrieved from GenBank. The species C. amycolatum presented an open pan-genome (α = 0.854905), with 3,280 gene families, being 1,690 (51.52%) in the core genome, 1,121 related to accessory genes (34.17%), and 469 related to unique genes (14.29%). Although some classic corynebacterial virulence factors are absent in the species C. amycolatum, we did identify genes associated with immune evasion, toxin, and antiphagocytosis among the predicted putative virulence factors. Additionally, we found genomic evidence for extensive acquisition of antimicrobial resistance genes through genomic islands."],["dc.identifier.doi","10.3389/fmicb.2022.1011578"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/117899"],["dc.language.iso","en"],["dc.relation.eissn","1664-302X"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Plant Science"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Leidenfrost, Robert M."],["dc.contributor.author","Bänsch, Svenja"],["dc.contributor.author","Prudnikow, Lisa"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Westphal, Catrin"],["dc.contributor.author","Wünschiers, Röbbe"],["dc.date.accessioned","2021-04-14T08:26:59Z"],["dc.date.available","2021-04-14T08:26:59Z"],["dc.date.issued","2020"],["dc.description.abstract","Bumble bees are important crop pollinators and provide important pollination services to their respective ecosystems. Their pollen diet and thus food preferences can be characterized through nucleic acid sequence analysis. We present ITS2 amplicon sequence data from pollen collected by bumble bees. The pollen was collected from six different bumble bee colonies that were placed in independent agricultural landscapes. We compared next-generation (Illumina), i.e., short-read, and third-generation (Nanopore), i.e., MinION, sequencing techniques. MinION data were preprocessed using traditional and Nanopore specific tools for comparative analysis and were evaluated in comparison to short-read sequence data with conventional processing. Based on the results, the dietary diary of bumble bee in the studied landscapes can be identified. It is known that short reads generated by next-generation sequencers have the advantage of higher quality scores while Nanopore yields longer read lengths. We show that assignments to taxonomic units yield comparable results when querying against an ITS2-specific sequence database. Thus, lower sequence quality is compensated by longer read lengths. However, the Nanopore technology is improving in terms of data quality, much cheaper, and suitable for portable applications. With respect to the studied agricultural landscapes we found that bumble bees require higher plant diversity than only crops to fulfill their foraging requirements."],["dc.identifier.doi","10.3389/fpls.2020.00287"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17385"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82133"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-462X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Analyzing the Dietary Diary of Bumble Bee"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Carvalho, Rodrigo"],["dc.contributor.author","Aburjaile, Flavia"],["dc.contributor.author","Canario, Marcus"],["dc.contributor.author","Nascimento, Andréa M. A."],["dc.contributor.author","Chartone-Souza, Edmar"],["dc.contributor.author","de Jesus, Luis"],["dc.contributor.author","Zamyatnin, Andrey A."],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Barh, Debmalya"],["dc.contributor.author","Ghosh, Preetam"],["dc.contributor.author","Goes-Neto, Aristoteles"],["dc.contributor.author","Figueiredo, Henrique C. P."],["dc.contributor.author","Soares, Siomar"],["dc.contributor.author","Ramos, Rommel"],["dc.contributor.author","Pinto, Anne"],["dc.contributor.author","Azevedo, Vasco"],["dc.date.accessioned","2021-04-14T08:29:51Z"],["dc.date.available","2021-04-14T08:29:51Z"],["dc.date.issued","2021"],["dc.description.abstract","The rapid emergence of multidrug-resistant (MDR) bacteria is a global health problem. Mobile genetic elements like conjugative plasmids, transposons, and integrons are the major players in spreading resistance genes in uropathogenic Escherichia coli (UPEC) pathotype. The E. coli BH100 strain was isolated from the urinary tract of a Brazilian woman in 1974. This strain presents two plasmids carrying MDR cassettes, pBH100, and pAp, with conjugative and mobilization properties, respectively. However, its transposable elements have not been characterized. In this study, we attempted to unravel the factors involved in the mobilization of virulence and drug-resistance genes by assessing genomic rearrangements in four BH100 sub-strains (BH100 MG2014, BH100 MG2017, BH100L MG2017, and BH100N MG2017). Therefore, the complete genomes of the BH100 sub-strains were achieved through Next Generation Sequencing and submitted to comparative genomic analyses. Our data shows recombination events between the two plasmids in the sub-strain BH100 MG2017 and between pBH100 and the chromosome in BH100L MG2017. In both cases, IS3 and IS21 elements were detected upstream of Tn21 family transposons associated with MDR genes at the recombined region. These results integrated with Genomic island analysis suggest pBH100 might be involved in the spreading of drug resistance through the formation of resistance islands. Regarding pathogenicity, our results reveal that BH100 strain is closely related to UPEC strains and contains many IS3 and IS21-transposase-enriched genomic islands associated with virulence. This study concludes that those IS elements are vital for the evolution and adaptation of BH100 strain."],["dc.identifier.doi","10.3389/fmicb.2020.549254"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83007"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-302X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Genomic Characterization of Multidrug-Resistant Escherichia coli BH100 Sub-strains"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]