Brenig, Bertram B.

[["dc.bibliographiccitation.firstpage","388"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Jürs, Simone"],["dc.contributor.author","Brem, Gottfried"],["dc.date.accessioned","2019-07-10T08:12:49Z"],["dc.date.available","2019-07-10T08:12:49Z"],["dc.date.issued","1990"],["dc.identifier.fs","28120"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4095"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61048"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0305-1048"],["dc.relation.issn","1362-4962"],["dc.relation.orgunit","Fakultät für Agrarwissenschaften"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","630"],["dc.title","The porcine PHIcDNA linked to the halothane gene detects a HindIII and XbaI RFLP in normal and malignant hyperthermia susceptible pigs."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","388"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Jürs, Simone"],["dc.contributor.author","Brem, Gottfried"],["dc.date.accessioned","2019-07-10T08:12:49Z"],["dc.date.available","2019-07-10T08:12:49Z"],["dc.date.issued","1990"],["dc.identifier.fs","28119"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4096"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61049"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0305-1048"],["dc.relation.issn","1362-4962"],["dc.relation.orgunit","Fakultät für Agrarwissenschaften"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","630"],["dc.title","The porcine PHIcDNA linked to the halothane gene detects a NotI RFLP in normal and malignant hyperthermia susceptible pigs."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5441"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Brem, Gottfried"],["dc.date.accessioned","2019-07-10T08:12:49Z"],["dc.date.available","2019-07-10T08:12:49Z"],["dc.date.issued","1991"],["dc.identifier.fs","28116"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4097"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61050"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0305-1048"],["dc.relation.issn","1362-4962"],["dc.relation.orgunit","Fakultät für Agrarwissenschaften"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","630"],["dc.title","Direct cloning of sequence tagged microsatellite sites by DNA affinity chromatography."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","14"],["dc.bibliographiccitation.journal","BMC veterinary research"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Bornemann-Kolatzki, Kirsten"],["dc.contributor.author","Knorr, Christoph"],["dc.contributor.author","Taeubert, Helge"],["dc.contributor.author","Brenig, Bertram"],["dc.date.accessioned","2019-07-09T11:52:29Z"],["dc.date.available","2019-07-09T11:52:29Z"],["dc.date.issued","2006"],["dc.description.abstract","Background: Inguinal hernias are usually caused by a congenital defect, which occurs as a weakness of the inguinal canal. Porcine â-glucuronidase gene (GUSB) was chosen as functional candidate gene because of its involvement in degradation of hyaluronan within gubernacular tissue during descent of testes. Since a genome-wide linkage analysis approach has shown evidence that two regions on porcine chromosome 3 (SSC 3) are involved in the inheritance of hernia inguinalis/scrotalis in German pig breeds, GUSB also attained status as a positional candidate gene by its localization within a hernia-associated chromosomal region. Results: A contig spanning 17,157 bp, which contains the entire GUSB, was assembled. Comparative sequence analyses were conducted for the GUSB gene locus. Single nucleotide polymorphisms (SNPs) located within the coding region of GUSB were genotyped in 512 animals. Results of transmission disequilibrium test (TDT) for two out of a total of five detected SNPs gave no significant association with the outcome of hernia in pigs."],["dc.identifier.doi","10.1186/1746-6148-2-14"],["dc.identifier.fs","38886"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/4401"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60201"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","630"],["dc.subject.ddc","636"],["dc.title","Molecular characterization and exclusion of porcine GUSB as a candidate gene for congenital hernia inguinalis/scrotalis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6422"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Müller, Mathias"],["dc.contributor.author","Brem, Gottfried"],["dc.date.accessioned","2019-07-10T08:12:48Z"],["dc.date.available","2019-07-10T08:12:48Z"],["dc.date.issued","1989"],["dc.description.abstract","Screening of a large number of transgenic animals is usually very time consuming and labourious. Quite a number of protocols have already been adopted to this problem including the polymerase chain reaction on different preimplantation embryos (1,2). We extremely simplified the standard procedures (3) by omitting unnecessary steps (phenol/chloroform extraction, precipitation) and optimizing several critical time parameters. The two protocols presented here are routinely used in our laboratory and start from minimal amounts (10-50 mg) of tissue (tail tip, skin scrapings). The DNA is extracted in an appropriate volume (50-100 ul) of 50 mM Tris-HCl pH 8.0/100 mM EDTA/100 mM NaCl/1 % SDS/500 ug/ml proteinase K for 2-4 hours at 58 °C with occasional mild shaking. In the first procedure (Fig. 1) DNA is electrophoresed (0.7 % agarose, ca. 0.5 h) directly after lysis of the tissue just until the bromphenolblue dye has entered the gel without a previous phenol/chloroform extraction. The gel is not covered with electrophoresis buffer while loading the samples and during the run. The DNA is transferred onto nylonmembranes using a vacuum blotting system (1 h) and hybridized as described (4). In the second procedure DNA is treated with phenol/chloroform once, the aequeous phase is heat-denatured for 5 min directly after extraction without precipitation and blotted onto nylonmembranes using a slot blot apparatus (5). In both protocols results are achieved within 25-35h essentially depending on the time of hybridization (10-16h) and exposure (2-16h) of the membranes."],["dc.identifier.fs","28122"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4014"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61044"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0305-1048"],["dc.relation.orgunit","Fakultät für Agrarwissenschaften"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","630"],["dc.title","A fast detection protocol for screening large numbers of transgenic animals."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]