Jensen, Nickels A.

[["dc.bibliographiccitation.artnumber","4762"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Kamper, Maria"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Jensen, Nickels A."],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2019-07-09T11:50:56Z"],["dc.date.available","2019-07-09T11:50:56Z"],["dc.date.issued","2018"],["dc.description.abstract","The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein."],["dc.identifier.doi","10.1038/s41467-018-07246-2"],["dc.identifier.pmid","30420676"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16027"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59855"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Near-infrared STED nanoscopy with an engineered bacterial phytochrome"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Communications Biology"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Jensen, Nickels"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Chizhik, Alexey I."],["dc.date.accessioned","2021-03-05T08:58:32Z"],["dc.date.available","2021-03-05T08:58:32Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1038/s42003-020-01316-2"],["dc.identifier.pmid","33128009"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17780"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80175"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/87"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.notes.intern","Merged from goescholar"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2399-3642"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Enderlein"],["dc.relation.workinggroup","RG Jakobs (Structure and Dynamics of Mitochondria)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","655"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","663"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Lavoie-Cardinal, Flavie"],["dc.contributor.author","Jensen, Nickels A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Chmyrov, Andriy"],["dc.contributor.author","Bierwagen, Jakob"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:46:25Z"],["dc.date.available","2017-09-07T11:46:25Z"],["dc.date.issued","2014"],["dc.description.abstract","Up to now, all demonstrations of reversible saturable optical fluorescence transitions (RESOLFT) superresolution microscopy of living cells have relied on the use of reversibly switchable fluorescent proteins (RSFP) emitting in the green spectral range. Here we show RESOLFT imaging with rsCherryRev1.4, a new red-emitting RSFP enabling a spatial resolution up to four times higher than the diffraction barrier. By co-expressing green and red RSFPs in living cells we demonstrate two-color RESOLFT imaging both for single (donut) beam scanning and for parallelized versions of RESOLFT nanoscopy where an array of >23000 donut-like minima are scanned simultaneously."],["dc.identifier.doi","10.1002/cphc.201301016"],["dc.identifier.gro","3142168"],["dc.identifier.isi","000332747500013"],["dc.identifier.pmid","24449030"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12826"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5288"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1439-7641"],["dc.relation.issn","1439-4235"],["dc.rights","CC BY-NC-ND 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/3.0"],["dc.title","Two-Color RESOLFT Nanoscopy with Green and Red Fluorescent Photochromic Proteins"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Plant Direct"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Ghareeb, Hassan"],["dc.contributor.author","Frahm, Lars"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Jensen, Nickels"],["dc.contributor.author","Teichmann, Thomas"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lipka, Volker"],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2021-04-14T08:23:40Z"],["dc.date.available","2021-04-14T08:23:40Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract Subdiffraction super‐resolution fluorescence microscopy, or nanoscopy, has seen remarkable developments in the last two decades. Yet, for the visualization of plant cells, nanoscopy is still rarely used. In this study, we established RESOLFT nanoscopy on living green plant tissue. Live‐cell RESOLFT nanoscopy requires and utilizes comparatively low light doses and intensities to overcome the diffraction barrier. We generated a transgenic Arabidopsis thaliana plant line expressing the reversibly switchable fluorescent protein rsEGFP2 fused to the mammalian microtubule‐associated protein 4 (MAP4) in order to ubiquitously label the microtubule cytoskeleton. We demonstrate the use of RESOLFT nanoscopy for extended time‐lapse imaging of cortical microtubules in Arabidopsis leaf discs. By combining our approach with fluorescence lifetime gating, we were able to acquire live‐cell RESOLFT images even close to chloroplasts, which exhibit very strong autofluorescence. The data demonstrate the feasibility of subdiffraction resolution imaging in transgenic plant material with minimal requirements for sample preparation."],["dc.identifier.doi","10.1002/pld3.261"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81007"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2475-4455"],["dc.relation.issn","2475-4455"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","Live‐cell RESOLFT nanoscopy of transgenic Arabidopsis thaliana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]