Orth, Alexander

[["dc.bibliographiccitation.firstpage","3104"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Chemical Science"],["dc.bibliographiccitation.lastpage","3114"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Schütte, Ole Mathis"],["dc.contributor.author","Ries, Annika"],["dc.contributor.author","Orth, Alexander"],["dc.contributor.author","Patalag, Lukas J."],["dc.contributor.author","Römer, Winfried"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Werz, Daniel B."],["dc.date.accessioned","2017-09-07T11:46:54Z"],["dc.date.available","2017-09-07T11:46:54Z"],["dc.date.issued","2014"],["dc.description.abstract","The Shiga toxin B subunit (STxB), which is involved in cell membrane attachment and trafficking of Shiga holotoxin, binds specifically to the glycosphingolipid Gb(3). In biological membranes, Gb(3) glycosphingolipids differ in their fatty acid composition and there is strong evidence that the fatty acid alters the binding behaviour of STxB as well as the intracellular routing of the Shiga toxin/Gb(3) complex. To analyse the binding of STxB to different Gb(3)s, we chemically synthesized saturated, unsaturated, alpha-hydroxylated Gb(3)s and a combination thereof, all based on a C-24-fatty acid chain starting from monosaccharide building blocks, sphingosine and the respective fatty acids. These chemically well-defined Gb(3)s were inserted into solid supported phase-separated lipid bilayers composed of DOPC/sphingomyelin/cholesterol as a simple mimetic of the outer leaflet of animal cell membranes. By fluorescence- and atomic force microscopy the phase behaviour of the bilayer as well as the lateral organization of bound STxB were analysed. The fatty acid of Gb(3) significantly alters the ratio between the ordered and disordered phase and induces a third intermediate phase in the presence of unsaturated Gb(3). The lateral organization of STxB on the membranes varies significantly. While STxB attached to membranes with Gb(3)s with saturated fatty acids forms protein clusters, it is more homogeneously bound to membranes containing unsaturated Gb(3)s. Large interphase lipid redistribution is observed for alpha-hydroxylated Gb(3) doped membranes. Our results clearly demonstrate that the fatty acid of Gb(3) strongly influences the lateral organization of STxB on the membrane and impacts the overall membrane organization of phase-separated lipid membranes."],["dc.identifier.doi","10.1039/c4sc01290a"],["dc.identifier.gro","3142208"],["dc.identifier.isi","000338652900019"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10662"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5732"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","2041-6539"],["dc.relation.issn","2041-6520"],["dc.rights.access","openAccess"],["dc.title","Influence of Gb(3) glycosphingolipids differing in their fatty acid chain on the phase behaviour of solid supported membranes: chemical syntheses and impact of Shiga toxin binding"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e6238"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","PLOS ONE"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Windschiegl, Barbara"],["dc.contributor.author","Orth, Alexander"],["dc.contributor.author","Römer, Winfried"],["dc.contributor.author","Berland, Ludwig"],["dc.contributor.author","Stechmann, Bahne"],["dc.contributor.author","Bassereau, Patricia"],["dc.contributor.author","Johannes, Ludger"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:46:52Z"],["dc.date.available","2017-09-07T11:46:52Z"],["dc.date.issued","2009"],["dc.description.abstract","The homopentameric B-subunit of bacterial protein Shiga toxin (STxB) binds to the glycolipid Gb(3) in plasma membranes, which is the initial step for entering cells by a clathrin-independent mechanism. It has been suggested that protein clustering and lipid reorganization determine toxin uptake into cells. Here, we elucidated the molecular requirements for STxB induced Gb(3) clustering and for the proposed lipid reorganization in planar membranes. The influence of binding site III of the B-subunit as well as the Gb(3) lipid structure was investigated by means of high resolution methods such as fluorescence and scanning force microscopy. STxB was found to form protein clusters on homogenous 1,2-dioleoyl-snglycero-3- phosphocholine (DOPC)/cholesterol/Gb(3) (65:30:5) bilayers. In contrast, membranes composed of DOPC/cholesterol/sphingomyelin/Gb(3) (40: 35: 20: 5) phase separate into a liquid ordered and liquid disordered phase. Dependent on the fatty acid composition of Gb(3), STxB-Gb(3) complexes organize within the liquid ordered phase upon protein binding. Our findings suggest that STxB is capable of forming a new membrane phase that is characterized by lipid compaction. The significance of this finding is discussed in the context of Shiga toxin-induced formation of endocytic membrane invaginations."],["dc.identifier.doi","10.1371/journal.pone.0006238"],["dc.identifier.gro","3143085"],["dc.identifier.isi","000268036000002"],["dc.identifier.pmid","19606209"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5853"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/560"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Lipid Reorganization Induced by Shiga Toxin Clustering on Planar Membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]