Flux, Anna Lena

[["dc.bibliographiccitation.artnumber","43"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Diversity"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Flux, Anna Lena"],["dc.contributor.author","Mazanec, Janine"],["dc.contributor.author","Strommenger, Birgit"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2019-07-09T11:44:36Z"],["dc.date.available","2019-07-09T11:44:36Z"],["dc.date.issued","2017"],["dc.description.abstract","Staphylococcus aureus is a major pathogen causing osteomyelitis, amongst other diseases, and its methicillin-resistant form (MRSA) in particular poses a huge threat to public health. To increase our knowledge of the origin and evolution of S. aureus, genetic studies of historical microorganisms may be beneficial. Thus, the aim of this study was to investigate whether osteomyelitic skeletal material (autopsy specimens collected from the mid 19th century until the 1920s) is suitable for detecting historical S. aureus DNA sequences. We established a PCR-based analysis system targeting two specific genes of S. aureus (nuc and fib). We successfully amplified the historical S. aureus nuc and fib sequences for six and seven pre-antibiotic, osteomyelitic bone specimens, respectively. These results encourage further investigations of historical S. aureus genomes that may increase our understanding of pathogen evolution in relation to anthropogenically introduced antibiotics."],["dc.identifier.doi","10.3390/d9040043"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14836"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59047"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1424-2818"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Staphylococcus aureus Sequences from Osteomyelitic Specimens of a Pathological Bone Collection from Pre-Antibiotic Times"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","113"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","BioTechniques"],["dc.bibliographiccitation.lastpage","122"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Flux, Anna Lena"],["dc.contributor.author","Schultz, Michael"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2022-10-04T10:22:04Z"],["dc.date.available","2022-10-04T10:22:04Z"],["dc.date.issued","2022"],["dc.description.abstract","For microscopic investigation, archaeological bone samples are often embedded in Biodur\n ®\n epoxy resin. This study wants to test whether it is possible to extract DNA suitable for PCR amplification from this sample type. For eight individuals a set of samples – each consisting of a Biodur-embedded femur sample, a native femur sample and a control sample of different anatomical origin – were submitted to organic DNA extraction. The extraction success was tested by autosomal short tandem repeat amplification. Seven out of eight Biodur-embedded femur samples revealed successful amplification results. If Biodur-embedded bone material exists from earlier microscopic investigations, our results encourage the use of this sample type as a source for genetic research."],["dc.description.abstract","Method summary\n \n An organic DNA extraction was performed on archaeological bone samples (femur) embedded in epoxy resin (Biodur\n ®\n ). The DNA extracts were suitable for STR typing of chromosomal DNA."],["dc.identifier.doi","10.2144/btn-2022-0056"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114582"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","1940-9818"],["dc.relation.issn","0736-6205"],["dc.title","Ancient DNA analysis from epoxy resin Biodur\n ®\n -embedded bones"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]